钛表面粗糙度对成纤维细胞生长影响的研究进展

doi:10.13701/j.cnki.kqyxyj.2019.08.004

摘要/Abstract

摘要： 种植体与结缔组织的成功结合对种植体远期成功起了至关重要的作用。良好的软组织结合与纯钛表面粗糙度关系密切,而软组织中成纤维细胞在种植体表面的粘附及增殖是建立以及维持良好的黏膜封闭的关键部分。因此近年来,研究人员探索了众多纯钛表面粗糙度与成纤维细胞的体内外实验,并得到了不同的结果。本文就近年来纯钛种植体表面粗糙度对于成纤维细胞生长影响的研究进展作一综述,为今后口腔种植领域的发展提供一定的理论依据。

关键词: 纯钛种植体, 表面形貌, 粗糙度, 成纤维细胞, 软组织封闭

Abstract: Successful integration of dental implant with gingival connective tissue plays an extremely crucial role on the longtime survival of the implant. Good soft tissue sealing is related to the roughness of pure titanium surface. The adhesion and proliferation of fibroblasts on the implant surface in soft tissue is the key to establish and maintain a good mucosal sealing. In recent years, researchers have performed many in vitro and in vivo experiments to study the relationship between surface roughness of pure titanium and fibroblasts. In this paper, the research progress of the influence of surface roughness of pure titanium implants on the growth of fibroblasts is reviewed, with aim to provide a theoretical basis for the future development of oral implant.

Key words: pure titanium implant, surface topography, roughness, fibroblasts, soft tissue sealing

江瑶, 黎红. 钛表面粗糙度对成纤维细胞生长影响的研究进展[J]. 口腔医学研究, 2019, 35(8): 738-740.

JIANG Yao, LI Hong. Research Progress on Effects of Titanium Surface Roughness on Fibroblast Growth[J]. Journal of Oral Science Research, 2019, 35(8): 738-740.

参考文献

[1] 陈江,周麟.种植体颈部设计的研究现状[J].口腔疾病防治,2016, 24(8): 441-444.
[2] Al RezkF, Trimpou G, Lauer HC, et al. Response of soft tissue to different abutment materials with different surface topographies: a review of the literature [J]. Gen Dent, 2018, 66(1): 18-25.
[3] 王闻天,孟维艳,周延民,等.不同钛合金表面形貌对成纤维细胞生物学行为的影响[J].实用口腔医学杂志,2014,30(2): 151-155.
[4] Borges AM, Benetoli LO, Licinio MA, et al. Polymer films with surfaces unmodified and modified by non-thermal plasma as new substrates for cell adhesion [J]. Mater Sci Eng C Mater Biol Appl, 2013, 33(3): 1315-1324.
[5] Critchley DR. Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion [J]. Biochem Soc Trans, 2004, 32(Pt 5): 831-836.
[6] Mierke CT, Kollmannsberger P, Zitterbart DP, et al. Vinculin facilitates cell invasion into three-dimensional collagen matrices [J]. J Biol Chem, 2010, 285(17): 13121-13130.
[7] Mierke CT, Kollmannsberger P, Zitterbart DP, et al. Mechano-coupling and regulation of contractility by the vinculin tail domain [J]. Biophys J, 2008, 94(2): 661-670.
[8] Goldmann WH. Mechanotransduction and focal adhesions [J]. Cell Biol Int, 2012, 36(7): 649-652.
[9] 王燕,王国平,夏露.钛种植体表面粗糙度研究进展[J].中国口腔种植学杂志,2012,17(2): 90-94.
[10] Grossner-Schreiber B, Herzog M, Hedderich J, et al. Focal adhesion contact formation by fibroblasts cultured on surface-modified dental implants: an in vitro study [J]. Clin Oral Implants Res, 2006, 17(6): 736-745.
[11] Furuhashi A, Ayukawa Y, Atsuta I, et al. The difference of fibroblast behavior on titanium substrata with different surface characteristics [J]. Odontology, 2012, 100(2): 199-205.
[12] Gomez-Florit M, Ramis JM, Xing R, et al. Differential response of human gingival fibroblasts to titanium- and titanium-zirconium-modified surfaces [J]. J Periodontal Res, 2014, 49(4): 425-436.
[13] 包崇云.牙种植体穿龈部位表面微结构对生物密封的影响[J].口腔材料器械杂志, 2017, 26(3): 113-117.
[14] 张晓洁,孟维艳,柳忠豪,等.不同钛表面形貌对人牙龈成纤维细胞生物学行为的影响[J].中国口腔种植学杂志,2011,16(1): 8-10.
[15] 赵爽.不同钛表面形貌对人牙龈成纤维细胞附着及胶原沉积的影响[D].2011,吉林大学.
[16] Ramaglia L, Di Spigna G, Capece G, et al. Differentiation, apoptosis, and GM-CSF receptor expression of human gingival fibroblasts on a titanium surface treated by a dual acid-etched procedure [J]. Clin Oral Investig, 2015, 19(9): 2245-2253.
[17] Zetterqvist L, Feldman S, Rotter B, et al. A prospective, multicenter, randomized-controlled 5-year study of hybrid and fully etched implants for the incidence of peri-implantitis [J]. J Periodontol, 2010, 81(4): 493-501.
[18] 赵颖,韩石磊,李大鹏,等.微纳复合结构对成纤维细胞黏附增殖的影响[J].临床口腔医学杂志,2016,32(11): 646-649.
[19] Kim H, Murakami H, Chehroudi B, et al. Effects of surface topography on the connective tissue attachment to subcutaneous implants [J]. Int J Oral Maxillofac Implants, 2006, 21(3): 354-365.
[20] Hamilton DW, Chehroudi B, Brunette DM. Comparative response of epithelial cells and osteoblasts to microfabricated tapered pit topographies in vitro and in vivo [J]. Biomaterials, 2007, 28(14): 2281-2293.
[21] Mustafa K, Oden A, Wennerberg A, et al. The influence of surface topography of ceramic abutments on the attachment and proliferation of human oral fibroblasts [J]. Biomaterials, 2005, 26(4): 373-381.
[22] Wang Y, Zhang Y, Miron RJ. Health, maintenance, and recovery of soft tissues around implants [J]. Clin Implant Dent Relat Res, 2016, 18(3): 618-634.
[23] Linares A, Munoz F, Permuy M, et al. Soft tissue histomorphology at implants with a transmucosal modified surface. A study in minipigs [J]. Clin Oral Implants Res, 2015, 26(9): 996-1005.
[24] Eisenbarth E, Linez P, Biehl V, et al. Cell orientation and cytoskeleton organisation on ground titanium surfaces [J]. Biomol Eng, 2002, 19(2-6): 233-237.
[25] 王丽波,韦纪英.种植体表面特征对软组织附着的研究进展[J].现代医药卫生,2016,32(3): 405-406.
[26] 赖颖真,陈江,林珊.钛表面微沟槽形貌对人牙龈纤维细胞黏着斑蛋白表达的影响[J].上海口腔医学,2015,24(2): 182-187.
[27] 李家奇,孟维艳,周延民, 等.不同表面形貌的纯钛种植体对牙龈软组织界面影响的扫描电镜观察[J].吉林大学学报(医学版), 2014, 40(1): 102-108.
[28] Anselme K, Davidson P, Popa, AM, et al. The interaction of cells and bacteria with surfaces structured at the nanometre scale [J]. Acta Biomater, 2010, 6(10): 3824-3846.
[29] 张玉军.纯钛表面纳米浅凹对人牙龈成纤维细胞附着影响的实验研究[D].2016,山东大学.
[30] Somaiah C, Kumar A, Mawrie D, et al. Collagen promotes higher adhesion, survival and proliferation of mesenchymal stem cells [J]. PLoS One, 2015, 10(12): e0145068.
[31] Kato E, Sakurai K, Yamada M. Periodontal-like gingival connective tissue attachment on titanium surface with nano-ordered spikes and pores created by alkali-heat treatment [J]. Dent Mater, 2015, 31(5): e116-e130.
[32] Wang RC, Hsieh MC, Lee TM. Effects of nanometric roughness on surface properties and fibroblast's initial cytocompatibilities of Ti6Al4V [J]. Biointerphases, 2011, 6(3): 87.
[33] Al-Ahmad A, Wiedmann-Al-Ahmad M, Fackler A, et al. In vivo study of the initial bacterial adhesion on different implant materials [J]. Arch Oral Biol, 2013, 58(9): 1139-1147.
[34] 戚娟娟.不同表面种植体软组织袖口临床和组织学的比较研究[D].2012,南方医科大学.
[35] 韩艾芳,黄宝鑫,邓动梅,等.钛种植体表面粗糙度对细菌黏附的影响[J].临床口腔医学杂志,2014,30(5): 283-286.
[36] Luthen F, Lange R, Becker P, et al. The influence of surface roughness of titanium on beta1- and beta3-integrin adhesion and the organization of fibronectin in human osteoblastic cells [J]. Biomaterials, 2005, 26(15): 2423-2440.