基于宏基因组学探究种植体周围菌群组成与演替

doi:10.13701/j.cnki.kqyxyj.2025.10.008

口腔医学研究 ›› 2025, Vol. 41 ›› Issue (10): 883-889.DOI: 10.13701/j.cnki.kqyxyj.2025.10.008

基于宏基因组学探究种植体周围菌群组成与演替

刘益兵^1,2,3, 项雨霏^1,2,3#, 李佳殷^1,2,3, 郭佳婷^1,2,3, 梁尉^1,2,3, 李梦婷^1,2,3, 周硕^1,2,3, 崔宵璇^1,2,3, 周秦^1,2,3*

1.陕西省颅颌面精准医学研究重点实验室,西安交通大学口腔医院陕西西安 710004;
2.陕西省牙颌疾病临床研究中心,西安交通大学口腔医院陕西西安 710004;
3.西安交通大学口腔医院种植科陕西西安 710004

收稿日期:2024-12-13 出版日期:2025-10-28 发布日期:2025-10-23
通讯作者: *周秦,E-mail:zhouqin0529@126.com
作者简介:刘益兵(1995~ ),男,陕西宝鸡人,主治医师,硕士,研究方向:宏基因组学。项雨霏(1999~ ),女,陕西咸阳人,硕士在读,研究方向:口腔微生物学。#为共同第一作者
基金资助:
陕西省重点研发计划(编号:2023-YBSF-162)

Exploring Composition and Succession of Peri-implant Microbiota Based on Metagenomics

LIU Yibing^1,2,3, XIANG Yufei^1,2,3#, LI Jiayin^1,2,3, GUO Jiating^1,2,3, LIANG Wei^1,2,3, LI Mengting^1,2,3, ZHOU Shuo^1,2,3, CUI Xiaoxuan^1,2,3, ZHOU Qin^1,2,3*

1. Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China;
2. Clinical Research Center of Shaanxi Province for Dental and Maxillofacial Diseases, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China;
3. Department of Implant Dentistry, College of Stomatology, Xi’an Jiaotong University, Xi’an 710004, China

Received:2024-12-13 Online:2025-10-28 Published:2025-10-23

摘要/Abstract

摘要： 目的: 探究种植体周围微生物群落的构成和演替过程。方法: 分别在种植修复体戴牙后不同阶段(30 min、1周、2周、3周和4周),采集19例患者种植体周围和邻牙龈沟液样本,通过宏基因组测序技术,分析微生物群落组成。结果: 种植牙周围的物种多样性与丰度与天然牙相比较低。天然牙与种植牙不同组间在门水平、属水平、种水平分别有4、8、18个物种存在差异,对此差异贡献最大的属是放线菌属和二氧化碳噬纤维菌属。在属水平,种植牙4组中链球菌丰度均为最高;在种水平,相对丰度排名前200的物种在不同时间节点上的丰度变化规律较复杂。结论: 种植体周围在戴牙后1周已经形成相对复杂的微生物群,其中链球菌属是先锋物种。

关键词: 种植体, 口腔微生物, 宏基因组学

Abstract: Objective: To explore the composition and successional process of the microbial community surrounding dental implants. Methods: Samples of peri-implant and adjacent gingival crevicular fluid were collected from 19 patients at different stages after dental prosthesis placement (30 minutes, 1 week, 2 weeks, 3 weeks, and 4 weeks). The composition of the microbial community was analyzed using metagenomic sequencing technology. Results: The species diversity and abundance of dental implants were lower compared to natural teeth. There were 4, 8, and 18 species with differences between natural teeth and dental implants at the phylum, genus, and species levels, respectively. The genera Actinomyces and Capnocytophaga were the major contributors to these differences. At the genus level, Streptococcus was the most abundant genus in all four groups of dental implants; at the species level, the abundance change patterns of the top 200 species across different time points were complex. Conclusion: A relatively complex microbial community had formed around the implants one week after prosthesis placement, with Streptococcus being the pioneer species.

Key words: dental implant, oral microbiota, metagenomics

刘益兵, 项雨霏, 李佳殷, 郭佳婷, 梁尉李梦婷, 周硕, 崔宵璇, 周秦. 基于宏基因组学探究种植体周围菌群组成与演替[J]. 口腔医学研究, 2025, 41(10): 883-889.

LIU Yibing, XIANG Yufei, LI Jiayin, GUO Jiating, LIANG Wei, LI Mengting, ZHOU Shuo, CUI Xiaoxuan, ZHOU Qin. Exploring Composition and Succession of Peri-implant Microbiota Based on Metagenomics[J]. Journal of Oral Science Research, 2025, 41(10): 883-889.

参考文献

[1] Buser D, Sennerby L, De Bruyn H. Modern implant dentistry based on osseointegration: 50 years of progress, current trends and open questions [J]. Periodontol 2000, 2017, 73(1): 7-21.
[2] Koenig JE, Spor A, Scalfone N, et al. Succession of microbial consortia in the developing infant gut microbiome [J]. Proc Natl Acad Sci U S A, 2011, 108 Suppl 1(Suppl 1): 4578-4585.
[3] La Rosa PS, Warner BB, Zhou Y, et al. Patterned progression of bacterial populations in the premature infant gut [J]. Proc Natl Acad Sci U S A, 2014, 111(34): 12522-12527.
[4] Lim ES, Zhou Y, Zhao G, et al. Early life dynamics of the human gut virome and bacterial microbiome in infants [J]. Nat Med, 2015, 21(10): 1228-1234.
[5] Kennedy B, Peura S, Hammar U, et al. Oral microbiota development in early childhood [J]. Sci Rep, 2019, 9(1): 19025.
[6] Jervøe-Storm PM, Alahdab H, Koltzscher M, et al. Comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction [J]. J Periodontol, 2007, 78(5): 909-917.
[7] Li D, Luo R, Liu CM, et al. MEGAHIT v1.0: A fast and scalable metagenome assembler driven by advanced methodologies and community practices [J]. Methods, 2016, 102: 3-11.
[8] Al-Ahmad A, Muzafferiy F, Anderson AC, et al. Shift of microbial composition of peri-implantitis-associated oral biofilm as revealed by 16S rRNA gene cloning [J]. J Med Microbiol, 2018, 67(3): 332-340.
[9] Siqueira JF Jr, Rôças IN, Souto R, et al. Actinomyces species, streptococci, and Enterococcus faecalis in primary root canal infections [J]. J Endod, 2002, 28(3): 168-172.
[10] Vielkind P, Jentsch H, Eschrich K, et al. Prevalence of Actinomyces spp. in patients with chronic periodontitis [J]. Int J Med Microbiol, 2015, 305(7): 682-688.
[11] Mark Welch JL, Rossetti BJ, Rieken CW, et al. Biogeography of a human oral microbiome at the micron scale [J]. Proc Natl Acad Sci U S A, 2016, 113(6): E791-E800.
[12] Dige I, Raarup MK, Nyengaard JR, et al. Actinomyces naeslundii in initial dental biofilm formation [J]. Microbiology (Reading), 2009, 155(Pt 7): 2116-2126.
[13] Mombelli A, Mericske-Stern R. Microbiological features of stable osseointegrated implants used as abutments for overdentures [J]. Clin Oral Implants Res, 1990, 1(1): 1-7.
[14] Aas JA, Paster BJ, Stokes LN, et al. Defining the normal bacterial flora of the oral cavity [J]. J Clin Microbiol, 2005, 43(11): 5721-5732.
[15] Dewhirst FE, Chen T, Izard J, et al. The human oral microbiome [J]. J Bacteriol, 2010, 192(19): 5002-5017.
[16] Keijser BJ, Zaura E, Huse SM, et al. Pyrosequencing analysis of the oral microflora of healthy adults [J]. J Dent Res, 2008, 87(11): 1016-1020.
[17] Hartmann AC, Marhaver KL, Klueter A, et al. Acquisition of obligate mutualist symbionts during the larval stage is not beneficial for a coral host [J]. Mol Ecol, 2019, 28(1): 141-155.
[18] Teles FR, Teles RP, Uzel NG, et al. Early microbial succession in redeveloping dental biofilms in periodontal health and disease [J]. J Periodontal Res, 2012, 47(1): 95-104.

基于宏基因组学探究种植体周围菌群组成与演替

Exploring Composition and Succession of Peri-implant Microbiota Based on Metagenomics

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	卢冠凡, 杨加震, 桑志芹, 杨玉娥, 孙德刚, 滕飞. 恒牙深龋微生物菌群特征的宏基因组研究[J]. 口腔医学研究, 2025, 41(9): 767-772.
[2]	魏星, 张志宏. 不同骨质类型对下颌双植体种植覆盖义齿应力影响的三维有限元分析[J]. 口腔医学研究, 2025, 41(5): 395-399.
[3]	叶子桐, 唐倩, 尹清, 杨陆一. 腭前部第三腭皱区域的软硬组织特征研究[J]. 口腔医学研究, 2025, 41(4): 314-319.
[4]	李丛丛, 赵莉. 巨噬细胞介导的骨免疫在种植体植入后骨整合中的作用及其机制研究[J]. 口腔医学研究, 2025, 41(3): 189-194.
[5]	王远帆, 孙晓军. 修复体引导种植体周软组织成形和转移方式的研究进展[J]. 口腔医学研究, 2025, 41(2): 90-95.
[6]	张黄, 苗瑞婧, 范旭升, 黄婕, 王永武. 探究NLRP3炎症小体对人原代牙龈成纤维细胞炎症刺激的影响[J]. 口腔医学研究, 2025, 41(2): 128-133.
[7]	李家鹤, 魏凌飞, 曾妮, 柳忠豪. 渐进性负载对种植体骨结合影响效果评价[J]. 口腔医学研究, 2025, 41(2): 147-154.
[8]	陆璐云, 张薇, 陈志方, 闫威风. 应用锥形束CT评价微种植体压低上颌伸长磨牙的临床疗效及三维重建影像学分析[J]. 口腔医学研究, 2025, 41(1): 45-49.
[9]	孙毓言, 孟维艳. 种植体周围炎易感因素的研究进展[J]. 口腔医学研究, 2024, 40(5): 389-392.
[10]	王昊喆, 李磊. 种植体周围炎治疗中软组织封闭的再建立[J]. 口腔医学研究, 2024, 40(11): 945-949.
[11]	罗之琳, 曹颖光. 口腔种植机器人的精度及应用现状[J]. 口腔医学研究, 2024, 40(11): 950-955.
[12]	赵健, 史舒雅, 李超群, 李昕, 侯德强. 颧牙槽嵴下微种植体对骨性Ⅱ类错牙合畸形患者微笑美学效果的影响[J]. 口腔医学研究, 2024, 40(11): 967-971.
[13]	郑泽君, 张颖, 孙金梦, 魏亚楠, 丁晓玲, 丁刚. Er:YAG激光与甘氨酸龈下喷砂治疗种植体周炎短期临床疗效的随机对照研究[J]. 口腔医学研究, 2024, 40(1): 56-60.
[14]	陈星霖, 陈思, 马文杰, 童昕. 原位点种植体再植的存留率及相关影响因素的回顾性分析[J]. 口腔医学研究, 2023, 39(8): 721-726.
[15]	薛盛豪, 吴美辰, 李健, 雷蕾, 刘中宁, 姜婷. CGRP体外促成骨、成神经效果及钛板表面CGRP缓释系统的建立[J]. 口腔医学研究, 2023, 39(6): 515-521.