肥胖复合牙周炎建模相关体重丢失的不对称问题

doi:10.13701/j.cnki.kqyxyj.2019.01.006

口腔医学研究 ›› 2019, Vol. 35 ›› Issue (1): 23-27.DOI: 10.13701/j.cnki.kqyxyj.2019.01.006

肥胖复合牙周炎建模相关体重丢失的不对称问题

余挺¹, 赵莉², 章锦才³, 轩东英^4*

1. 广州医科大学附属口腔医院牙周科·广州口腔疾病研究所·口腔医学重点实验室广东广州 510140;
2. 中山大学光华口腔医学院·附属口腔医院口腔修复科,广东省口腔医学重点实验室广东广州 510055;
3. 中国科学院大学,存济医学院北京 100049;
4. 中国科学院大学存济医学院,杭州口腔医院牙周科浙江杭州 310006

收稿日期:2018-05-28 出版日期:2019-01-18 发布日期:2019-01-28
通讯作者: 轩东英,E-mail:xuanxuan187@126.com
作者简介:余挺(1987~ ),男,博士,主治医师,从事牙周病学的医教研工作。
基金资助:
国家自然科学基金(编号:81700985、81371151、81470750);广州市卫生和计划生育科技项目(编号:20181A011102)

Imbalanced Post-operative Weight Loss in Combined Obesity and Periodontitis Models

YU Ting¹, ZHAO Li², ZHANG Jin-cai³, XUAN Dong-ying^4*

1. Department of Periodontology, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China;
2. Department of Prosthodontics, Guanghua School of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China;
3. Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China;
4. Department of Periodontology, Hangzhou Dental Hospital, Savaid Medical School, University of Chinese Academy of Sciences, Hangzhou 310006, China

Received:2018-05-28 Online:2019-01-18 Published:2019-01-28

摘要/Abstract

摘要： 目的:探索肥胖复合牙周炎建模的主要实验因素对结扎后体重丢失(POWL)的影响。方法:建立饮食诱导型肥胖与结扎诱导牙周炎的小鼠复合模型。四因素方差分析判别饲料期(8、16、30周)、饲料、结扎、结扎期(5、10 d)对POWL的效应。结果:肥胖小鼠、牙周炎小鼠的POWL比各自对照组分别高70%(10.4% vs. 6.1%,P<0.001)、30%(9.6% vs. 7.4%,P=0.002)。随着肥胖程度增加,肥胖小鼠的POWL不断加重(P<0.001),与对照组的POWL差距拉大,且丢失体重恢复更困难。在特定饲料期(16周),POWL随结扎期延长而加重。结论:建立肥胖复合牙周炎模型时,POWL在主要实验因素(肥胖及其程度、牙周炎及其程度)的组内分布均不对称,应慎重评估并弱化其对实验目标的潜在干扰。

关键词: 混杂效应, 肥胖, 牙周炎, 体重丢失

Abstract: Objective: To explore the effects of main experimental factors on post-operative weight loss (POWL) when establishing combined obesity and periodontitis models. Methods: Combined models of diet-induced obesity and ligation-induced periodontitis were established. Effects of feeding duration (8, 16 and 30 weeks), diet, ligation and ligation duration (5 and 10 days) on POWL were evaluated by 4-way ANOVA. Results: DIO and periodontitits mice had 70% (10.4% vs. 6.1%, P<0.001) and 30% (9.6% vs. 7.4%, P=0.002) higher POWL, respectively, in contrast to their corresponding controls. As severity of obesity increased, POWL of the DIO mice was elevated and more difficult to recover and POWL difference between DIO mice and the controls was further enhanced. More POWL went with longer ligation duration after specific feeding duration (16 weeks). Conclusion: When establishing combined obesity and periodontitis models, imbalanced POWL occurs within groups of the main experimental factors (including obesity and periodontitis and their severity), which should be critically assessed to minimize its potential disturbance on experimental objectives.

Key words: Confounding effect, Obesity, Periodontal diseases, Weight loss

余挺, 赵莉, 章锦才, 轩东英. 肥胖复合牙周炎建模相关体重丢失的不对称问题[J]. 口腔医学研究, 2019, 35(1): 23-27.

YU Ting, ZHAO Li, ZHANG Jin-cai, XUAN Dong-ying. Imbalanced Post-operative Weight Loss in Combined Obesity and Periodontitis Models[J]. Journal of Oral Science Research, 2019, 35(1): 23-27.

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肥胖复合牙周炎建模相关体重丢失的不对称问题

Imbalanced Post-operative Weight Loss in Combined Obesity and Periodontitis Models

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