微纳复合结构钛涂层的制备及其生物相容性研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (12): 1197-1200.

微纳复合结构钛涂层的制备及其生物相容性研究

刘滋川¹,李雅静¹,孙亚杰¹,谢琪²,杜青³,谢伟丽^1*

1. 哈尔滨医科大学口腔医学院修复科黑龙江哈尔滨 150001;
2. 哈尔滨市儿童医院口腔科黑龙江哈尔滨 150010;
3. 哈尔滨工业大学材料科学与工程学院黑龙江哈尔滨 150001

收稿日期:2015-08-11 出版日期:2015-12-28 发布日期:2016-03-21
通讯作者: 谢伟丽,电话:0451-85553231
作者简介:刘滋川(1990~),男,汉族,安徽省宿州人,硕士,医师,主要从事口腔生物材料学方面的研究。
基金资助:
黑龙江省科技攻关项目(编号:GC12C305-3)

Study on the Fabrication and Biocompatibility of Hierarchical Titanium Surface.

LIU Zi-chuan¹, LI Ya-jing¹, SUN Ya-jie¹, XIE Qi², DU Qing³, XIE Wei-li¹.

1. Department of Prosthodontics, College of Stomatology, Harbin Medical University, Harbin 150001, China;
2. Department of Stomatology, Harbin Children's Hospital, Harbin 150010, China;
3. School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China

Received:2015-08-11 Online:2015-12-28 Published:2016-03-21

摘要/Abstract

摘要： 目的:在钛表面构建微纳复合结构涂层,并对其表面结构及生物相容性进行初步研究。方法:通过喷砂酸蚀、混合碱热处理的复合工艺,在钛表面构建新型微纳复合涂层,使用扫描电子显微镜(SEM)及X-射线能量分散分光计(EDS)对试件表征进行观察;使用CCK-8试剂盒检测该涂层的细胞毒性;进一步检测该涂层对于前成骨细胞(MC3T3-E1)粘附与增殖活性的影响。结果:钛表面微纳复合结构中,微米孔直径3~5 μm,纳米孔直径100~200 nm;新型涂层表面增加了钠元素和钙元素;体外细胞存活率实验表明该涂层细胞毒性0级;体外细胞粘附实验表明:第1、2 h,细胞粘附活性在喷砂酸蚀处理组(MTi组)及混合碱处理组(NTi组)均明显强于单纯抛光组(STi组),同时NTi组最强。体外细胞增殖实验表明:第1、4、7天MTi组的A值均小于STi组,具有统计学差异。NTi组的A值先是小于,后逐渐大于STi组。结论:相较于单独微米结构钛涂层,微纳复合结构钛涂层具有更接近于自然骨的分级结构以及更优良的生物活性。

关键词: 钛表面, 微纳复合结构, 细胞毒性, 细胞粘附, 细胞增殖

Abstract: Objective: To fabricate micro/nanostructures on titanium surface and analyze effects of hierarchical titanium surface on its biocompatibility. Methods: Mixed alkali treatment on the titanium surface was conducted after sandblast and acid etching, then scanning electronic microscope (SEM) and the X-ray energy dispersive spectrometer (EDS) were used to investigate microstructures of the titanium coatings. Survival rate of L-929 cells were detected by CCK-8 kit. The adherent cells of each group were stained by DAPI, while the proliferation of preosteoblast of each group was investigated further. Results: On the hierarchical structures of titanium surface, the scale of micro pit was about 3-5μm, while the nanostructure was about 100-200nm. Sodium and calcium were found on the new type surface. Cell toxicity was Class 0. Adhesion assay showed that the number of adherent cells both on MTi and NTi were bigger than that of STi, while cells on NTi were most at 1 hour or 2 hours. Proliferation test displayed that the proliferation activity on MTi was significantly lower than that on STi at day 1, 4, 7, while the proliferation activity on NTi was higher than that on STi at day 7. Conclusion: Hierarchical structure shows more biocompatible structure and better activity of adhesion and proliferation.

刘滋川,李雅静,孙亚杰,谢琪,杜青,谢伟丽. 微纳复合结构钛涂层的制备及其生物相容性研究[J]. 口腔医学研究, 2015, 31(12): 1197-1200.

LIU Zi-chuan, LI Ya-jing, SUN Ya-jie, XIE Qi, DU Qing, XIE Wei-li.. Study on the Fabrication and Biocompatibility of Hierarchical Titanium Surface.[J]. Journal of Oral Science Research, 2015, 31(12): 1197-1200.

参考文献

[1] Lim YJ, Oshida Y, Andre CJ, et al. Surface characterization of variously treated titanium materials [J]. Int J Oral Maxillofac Implants, 2001, 16∶333-42
[2] Zhu X, Chen J, Scheideler L, et al. Effects of topography and composition of titanium surface oxides on osteoblast responses [J]. Biomaterials, 2004, 25(18)∶4087-103
[3] Liu H, Webster TJ. Nanomedicine for implants: A review of studies and necessary experimental tools [J]. Biomaterials, 2007, 28∶354-369
[4] Kim MJ, Kim CW, Lim YJ, et al. Microrough titanium surface affects biologic response in MG63 osteoblast-like cells [J]. J Biomed Mater Res A, 2006, 79∶1023-32
[5] Saito T, Hayashi H, Kameyama T, et al. Suppressed proliferation of mouse osteoblast-like cells by a rough-surfaced substrate leads to low differentiation and mineralization [J]. Mater Sci Eng C, 2010, 30∶1-7
[6] ISO10993-12:2012 Biological evaluation of medical devices -Part 12: Sample preparation and reference materials [S]. Switzerland, International Organization for Standardization, 2012
[7] ISO10993-5:2009 Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity [S]. Switzerland, International Organization for Standardization,2012
[8] Liu X, Li X, Li S, et al. An in vitro study of a titanium surface modified by simvastatin-loaded titania nanotubes-micelles [J]. Journal of biomedical nanotechnology, 2014, 10(2)∶194-204
[9] 袁坤,王富,高婧等.一种新型牙科二硅酸锂玻璃陶瓷的微观结构分析[J].口腔医学研究,2014,31 (12)∶1121-1123
[10] Schwartz Z, Olivares-Navarrete R, Wieland M,etal. Mechanisms regulating increased production of osteoprotegerin by osteoblasts cultured on microstructured titanium surfaces [J]. Biomaterials, 2009, 30∶3390-6
[11] 路萌萌,周延民, 赵静辉.成骨细胞粘附中整合素机制的研究进展[J].口腔医学研究,2014,30(5)∶479-481
[12] Zhou J, Li B, Lu S, Zhang L, Han Y. Regulation of osteoblast proliferation and differentiation by interrod spacing of Sr-HA nanorods on microporous titania coatings [J]. ACS applied materials & interfaces, 2013, 5(11)∶5358-65
[13] Lingzhou Zhao, Shenglin Mei, Paul K. Chu, et al. The influence of hierarchical hybrid micro/nano-textured titanium surface with titania nanotubes on osteoblast functions [J]. Biomaterials, 2010, 31∶5072-5082

[1]	邱吟枫, 汤颖, 沈忆芬, 刘超, 沈昊, 顾永春, 于金华. 氟暴露对人牙周膜干细胞增殖及成骨分化的影响[J]. 口腔医学研究, 2020, 36(9): 866-870.
[2]	尚佳鑫, 苏瑞, 马冲, 李新月. 两种抛光方式对两种充填材料表面形貌和细胞增殖能力的影响[J]. 口腔医学研究, 2020, 36(6): 581-584.
[3]	王薛藤, 聂敏海, 左东川, 邓浩, 曾锦. TRPM4通道对人口腔颊黏膜成纤维细胞增殖和迁移的影响[J]. 口腔医学研究, 2020, 36(5): 490-495.
[4]	张维甲, 潘爽, 李艳萍, 何丽娜, 吴作栋, 牛玉梅. 兔牙髓干细胞与雪旺细胞体外间接共培养对增殖和兔牙髓干细胞成神经向分化的研究[J]. 口腔医学研究, 2020, 36(10): 968-972.
[5]	黄奕智, 郭俊, 杨健. 富血小板纤维蛋白对牙周膜成纤维细胞成骨能力影响的实验研究[J]. 口腔医学研究, 2020, 36(1): 41-45.
[6]	程海燕, 沈兰花, 张瑞, 孟玲娜, 刘迪, 刑北昱, 陶冠男. 雌孕激素对人牙周膜细胞成骨分化影响的研究[J]. 口腔医学研究, 2019, 35(7): 657-660.
[7]	徐方方,殷丽华. 富血小板纤维蛋白对干细胞增殖及成骨分化的研究进展[J]. 口腔医学研究, 2019, 35(6): 527-530.
[8]	肖婉鲁,潘爽,侯婷婷,李艳萍,何丽娜,牛玉梅. 载柚皮苷胶原蛋白凝胶对人牙髓干细胞增殖的影响[J]. 口腔医学研究, 2019, 35(6): 541-545.
[9]	汪晓龙,曹顺顺,张鹏,舒传继,夏林. 人源性长寿保障基因2在口腔鳞癌组织中表达及对细胞增殖和侵袭能力的影响[J]. 口腔医学研究, 2019, 35(2): 142-146.
[10]	蔡彦坤, 郑国莹, 隋磊. 纯钛或钛合金种植材料表面不同纳米结构对细胞行为的影响[J]. 口腔医学研究, 2018, 34(7): 699-702.
[11]	周茜雯, 曾豪, 张嘉昕, 刘恭奇, 王飒, 汪烈. 箭根酮对LPS刺激牙周膜成纤维细胞增殖能力的影响[J]. 口腔医学研究, 2018, 34(6): 653-656.
[12]	史彦, 邓志鹏, 陈浩. 5种根管冲洗液对小鼠成纤维细胞L929的毒性评价[J]. 口腔医学研究, 2018, 34(4): 388-392.
[13]	张厚华, 范华俐. RNA干扰沉默钙蛋白酶1对口腔鳞状细胞癌细胞株HSC3增殖和迁移的影响[J]. 口腔医学研究, 2018, 34(2): 152-156.
[14]	裴浩, 夏冬景, 黄莹莹. STAT6在涎腺腺样囊性癌组织中的表达及对细胞增殖和侵袭的影响[J]. 口腔医学研究, 2018, 34(11): 1212-1216.
[15]	李毅萍, 戴小寒, 唐瞻贵, 张学慧. BaTO₃/P(VDF-TrFE)纳米复合膜对干细胞铺展与增殖的影响[J]. 口腔医学研究, 2018, 34(1): 6-9.