不同牙周状态下龈沟液中瘦素水平的比较

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (8): 798-.

不同牙周状态下龈沟液中瘦素水平的比较

刘崇武,吴春芳,骆凯,王希,陈超,郑瑜谦^*

福建医科大学附属口腔医院修复科福建福州 350002

收稿日期:2014-11-24 出版日期:2015-08-28 发布日期:2016-05-04
通讯作者: 郑瑜谦,E-mail: zhyuqian@sina.com
作者简介:刘崇武(1975~ ),男,福建人,副主任医师,学士,主要从事口腔修复学临床及基础的研究工作。
基金资助:
福建省自然科学基金计划项目(编号:2010J0101 );福建省卫生厅青年科研课题(编号:2010-1-38)

Comparison of Leptin Levels in Gingival Crevicular Fluid of Different Periodontal Status

LIU Chong-wu, WU Chun-fang, LUO Kai, et al

Department of Periodontology, The Affiliated Stomatology Hospital, Fujian Medical University, Fuzhou 350002

Received:2014-11-24 Online:2015-08-28 Published:2016-05-04

摘要/Abstract

摘要： 目的:检测牙周健康者、慢性龈炎和慢性牙周炎患牙龈沟液中瘦素水平,为牙周炎的早期诊断提供客观指标。方法:选择牙周健康者、慢性龈炎和慢性牙周炎患者3组,治疗前记录牙周各项临床指标,并用Whatman^ 1号滤纸收集颊侧近远中牙周袋内GCF采用ELISA检测瘦素含量;同时收集血清进行瘦素水平的检测。结果:3组中慢性牙周炎组龈沟液中瘦素水平与牙周健康者龈沟液中瘦素水平比较有显著差异(P<0.05),慢性牙周炎组龈沟液中瘦素水平与慢性龈炎龈沟液中瘦素水平及慢性龈炎组龈沟液中瘦素水平与牙周健康者龈沟液中瘦素水平比较均无显著差异;牙周健康者、慢性龈炎和慢性牙周炎患者血液中瘦素水平比较均有显著差异(P<0.05)。血中的瘦素与临床指标PLI (P<0.01,r=0.593)、PD(P<0.05,r=0.920)、BI(P<0.05,r=0.862)、AL(P<0.05,r=0.846)均相关;龈沟液中的瘦素与临床指标PLI(P<0.01,r=0.813)、PD(P<0.05,r=0.962))、BI(P<0.05,r=0.720、AL(P<0.05,r=0.946)均相关。结论:龈沟液中瘦素水平可能是反映牙周组织状况的一项较为客观的指标。

关键词: 牙周炎, 牙龈炎, 龈沟液, 瘦素

Abstract: Objective: To evaluate gingival crevicular fluid (GCF) leptin levels and their relationship with periodontal health and disease. Methods: Forty-two individuals were divided into three groups: healthy subjects, patients with chronic gingivitis and chronic periodontitis. For each subject, plaque index (PLI), pocket depth (PD), bleeding index (BI), and attachment level (AL) values were recorded before and after treatment. GCF samples (by Whatman^ 1 filter paper) and serum samples (by venipuncture) were respectively collected to determine the leptin levels using enzyme-linked immunosorbent assay (ELISA) kit. Results: The lowest GCF leptin level was observed in subjects with chronic periodontitis, and statistically significant compared with healthy subjects (P<0.05). In contrast, the highest serum leptin concentration was obtained for subjects with chronic periodontitis. The PLI, PD, BI, and AL all showed significant correlations with leptin levels in GCF and serum (P<0.05). Conclusion: Leptin levels in GCF may be used as an objective indicator to evaluate periodontal status.

Key words: Periodontitis, Gingivitis, Gingival crevicular fluid, Leptin

中图分类号:

R781.4

刘崇武,吴春芳,骆凯,王希,陈超,郑瑜谦. 不同牙周状态下龈沟液中瘦素水平的比较[J]. 口腔医学研究, 2015, 31(8): 798-.

LIU Chong-wu, WU Chun-fang, LUO Kai, et al. Comparison of Leptin Levels in Gingival Crevicular Fluid of Different Periodontal Status[J]. Journal of Oral Science Research, 2015, 31(8): 798-.

参考文献

[1] MacDougald OA, Hwang CS, Fan H, et al. Regulated expression of the obese gene product(1eptin)in white adipose tissue and 3T3-L1 adipocytes [J]. Proc Natl Acad Sci USA, 1995, 92(20)∶9034-9037
[2] Auwe J, Staels B. Leptin [J]. Lancet,1998,351(9104)∶737-742
[3] Considine RV, Sinha MK, Heiman ML, et al. Serum immuno-reactive-leptin concentrations in normal-weight and obese humans [J]. N Engl J Med, 1996, 334∶292-295
[4] Karthikeyan BV, Pradeep AR. Gingival crevicular fluid and serum leptin: theirrelationship to periodontal health and disease [J]. J Clin Periodontol, 2007, 34∶467-472
[5] Whipple T, Sharkey N, Demers L, et al. Leptin and the skeleton [J]. Clin Endocrinol (Oxf), 2002, 57(6)∶701-711
[6] Takeda S, Elefterio F, Levasseur R, et al. Leptin regulates bone formation via the sympathetic nervous system [J]. Cell, 2002, 111(3)∶306-317
[7] Silness J, Lǒe H. Periodontal disease in pregnancy Ⅱ. Correlation between oral hygiene and periodontitis condition [J]. Acta Odontol Scand,1964,22(2)∶121-135
[8] Mazza JE, Newman MG, Sima TN. Clinical And antimicrobial effect of stannous fluoride on periodontitis [J]. J Clin Periodontol,1981,8(3)∶203-212
[9] Socransky SS, Haffajec AD, Goodson JM, et al. New concept of destructive periodontal disease [J]. J Clin Periodontol, 1984, 11(1)∶21-32
[10] Matarese G, Moschos S, Mantzoros CS. Leptin in immunology [J]. J Immunol, 2005, 174(6)∶3137-3142
[11] Ay ZY1, Kirzioglu FY, Tongu MO, et al. The gingiva contains leptin and leptin receptor in health and disease [J]. Odontology, 2012,100(2)∶222-231
[12] John J, Taylor1, Philip M. Preshaw1 and Evanthia Lalla A review of the evidence for pathogenic mechanisms that may link periodontitis and diabetes [J]. Journal of Clinical Periodontology, 2013, 40(14)∶113-134

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