口腔医学研究

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (11): 1127-1131.DOI: 10.13701/j.cnki.kqyxyj.2016.11.004

• 基础研究论著 • 上一篇    下一篇

纯镁种植体超声微弧氧化-NaOH-硅烷-丝素改性的动物实验研究

刘继光,王赞,刘苗,李慕勤*   

  1. 佳木斯大学 黑龙江省高校生物医学材料重点实验室 黑龙江 佳木斯 154007
  • 收稿日期:2016-05-17 出版日期:2016-11-25 发布日期:2016-11-25
  • 通讯作者: 李慕勤,E-mail: jmsdxlimuqin@163.com
  • 作者简介:刘继光(1961~ ),男,黑龙江人,博士,教授,从事生物医学材料方面研究工作。
  • 基金资助:
    国家自然科学基金(编号:31370979)
    佳木斯大学研究生科技创新项目(编号:LZZ2014__023)
    佳木斯大学口腔病防治协同创新中心课题

Research of Pure Magnesium Micro-arc Oxidation-NaOH-silane Silk Fibroin Composited Coatings Implanted in Vivo

LIU Ji-guang, WANG Zan, LIU Miao, LI Mu-qin*   

  1. Key Biomedical Materials Laboratory of Colleges and Universities in Heilongjiang Province, Jiamusi University, Jiamusi 154007, China
  • Received:2016-05-17 Online:2016-11-25 Published:2016-11-25

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摘要： 目的:研究超声微弧氧化硅烷偶联丝素蛋白复合处理涂层对纯镁植入体骨整合与降解情况的影响。方法:将经过超声微弧氧化后的纯镁植入体NaOH处理,然后在其表面分别进行0.5 h及1.5 h的硅烷偶联丝素蛋白的复合处理,分别为组A、组B、组C,将其植入兔子下颌骨。结果:扫描电镜观察可见组C表面最为致密,血镁浓度显示经复合改性后血镁浓度变化较小,扭出实验显示各周组C＞组B＞组A,并具有统计学差异,HE染色和免疫组化及灰度值分析显示各周组C骨整合能力最强。结论:纯镁植入体经超声微弧氧化碱液(NaOH)处理及硅烷偶联丝素蛋白的复合改性后具有更理想的降解速率及更强的促进骨整合能力。

关键词: 纯镁, 硅烷偶联剂, 丝素蛋白, 微弧氧化, 动物实验

Abstract: Objective: To study the effects of ultrasonic micro arc oxidation silane coupled silk fibroin composite coating on the bone consolidation and degradation of pure magnesium implants. Methods: After ultrasonic micro arc oxidation, pure magnesium implants were treated with NaOH and then with silane coupled silk fibroin on the surface for 0.5 h or 1.5 h, which were divided into group A, group B and group C, respectively. The modified materials were implanted in the rabbit mandible. Results: Scanning electron microscope observation showed that the surface of group C was the most compact. The concentration of magnesium in blood showed little change after compound modification. Torsion experiment demonstrated that the group C > group B > group A, showing statistical difference. HE staining, immunohistochemistry and gray value analysis showed that the group C had the strongest ability of bone consolidation. Conclusion: Pure magnesium implant with ultrasonic micro arc oxidation of alkali (NaOH) processing and silane coupling and silk fibroin composite modification showed better degradation rate and stronger promotion capability of osseointegration.

Key words: Pure magnesium, Silane coupling agent, Silk, Fibroin, Micro arc oxidation, Animal experiments

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