降钙素基因相关肽对成骨细胞OPN表达的影响

doi:10.13701/j.cnki.kqyxyj.2016.11.006

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (11): 1137-1140.DOI: 10.13701/j.cnki.kqyxyj.2016.11.006

降钙素基因相关肽对成骨细胞OPN表达的影响

李源静,杨岚,李倩倩,孙千月,邓天,任文,伍彩娟,郭吕华^*

广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室广东广州 510140

收稿日期:2016-04-26 出版日期:2016-11-25 发布日期:2016-11-25
通讯作者: 郭吕华,E-mail:562210919@qq.com
作者简介:李源静(1988~ ),女,河南平顶山郏县人,硕士,住院医师,主要从事修复学和种植骨整合基础研究。
基金资助:
广东省科技计划项目(编号:2013B021800278)
荔湾区科技局项目(编号:20141216055)
广州医科大学青年科研项目(编号:2015A33,2015A32)

Effect of Calcitonin Gene-related Peptide on OPN Expression in Osteoblasts

LI Yuan-jing, YANG Lan, LI Qian-qian, SUN Qian-yue, DENG Tian, REN Wen, WU Cai-juan, GUO Lv-hua^*

Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China

Received:2016-04-26 Online:2016-11-25 Published:2016-11-25

摘要/Abstract

摘要： 目的:探讨外源性降钙素基因相关肽(Calcitonin gene related peptide, CGRP)对大鼠成骨细胞中骨桥蛋白(Osteopontin,OPN)表达的影响。方法:胶原酶消化法培养大鼠原代成骨细胞,碱性磷酸酶染色及茜素红染色对成骨细胞进行鉴定。CCK-8细胞增殖实验筛选CGRP最适作用浓度。将合适浓度的CGRP作用于成骨细胞,qPCR和Western Blot检测OPNmRNA及蛋白的表达。结果:CGRP浓度为10^-9 mol/L时,大鼠成骨细胞的增殖活性最强。使用10^-9 mol/L浓度的CGRP作用于成骨细胞,成骨细胞中OPN mRNA及蛋白的表达在第3天明显上调(P<0.05)。结论:CGRP可以促进成骨细胞中OPN的表达。

关键词: 降钙素基因相关肽, 骨桥蛋白, 成骨细胞

Abstract: Objective: To investigate the effects of Calcitonin gene related peptide on OPN expression in primary cultured rat osteoblasts. Methods: Primary cultured rat osteoblasts were obtained by trypsin and collagenase digestion way from calvarial bone of neonate rats and were identified by ALP staining and alizarin red staining. The appropriate concentration of CGRP was selected by CCK-8 assay.The expression of OPN were quantified by qPCR and Western blot after cultured with CGRP. Results: The proliferation activity of osteoblasts enhanced when the concentration of CGRP was 10^-9mol/L. The expression of OPN mRNA increased on the third day(P<0.05), and the expression of OPN protein was consistent with the change of the OPN mRNA. Conclusion: CGRPcould promote the proliferation of osteoblasts when the concentration of CGRP was 10^-9mol/L. CGRP could promote OPN expression in the primary cultured rat osteoblasts.

Key words: Calcitonin gene-related peptide, Osteopontin, Osteoblast

中图分类号:

R780.1

李源静,杨岚,李倩倩,孙千月,邓天,任文,伍彩娟,郭吕华. 降钙素基因相关肽对成骨细胞OPN表达的影响[J]. 口腔医学研究, 2016, 32(11): 1137-1140.

LI Yuan-jing, YANG Lan, LI Qian-qian, SUN Qian-yue, DENG Tian, REN Wen, WU Cai-juan, GUO Lv-hua. Effect of Calcitonin Gene-related Peptide on OPN Expression in Osteoblasts[J]. Journal of Oral Science Research, 2016, 32(11): 1137-1140.

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降钙素基因相关肽对成骨细胞OPN表达的影响

Effect of Calcitonin Gene-related Peptide on OPN Expression in Osteoblasts

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