A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响

doi:10.13701/j.cnki.kqyxyj.2017.04.023

口腔医学研究 ›› 2017, Vol. 33 ›› Issue (4): 440-443.DOI: 10.13701/j.cnki.kqyxyj.2017.04.023

A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响

张晓艳^1,2, 程俊³, 许华丽^1,2, 毛亮³, 聂敏海^1,2*

1. 西南医科大学附属口腔医院口腔内科四川泸州 646000;
2. 西南医科大学口颌面修复重建和再生实验室四川泸州 646000;
3. 西南医科大学电生理学教育部重点实验室四川泸州 646000

收稿日期:2016-09-28 出版日期:2017-04-20 发布日期:2017-04-24
通讯作者: 聂敏海,电话:18982772207
作者简介:张晓艳(1991~ ),女,四川凉山人,医师,硕士在读,主要从事黏膜病病因的防治研究。
基金资助:
四川省应用基础研究计划项目(编号:LY-62)

Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells.

ZHANG Xiao-yan^1,2, CHENG Jun³, XU Hua-li^1,2, MAO Liang³, NIE Min-hai^1,2*.

1. Dept. of Oral Medicine, The Affiliated Hospital of Stomatology, Southwest Medical University. Luzhou 646000, China;
2. Laboratory of Oral and Maxillofacial Reconstruction and Regeneration, Institute of Cardiovascular Medicine, Southwest Medical University. Luzhou 646000, China;
3. Key Laboratory of Medical Electrophysiology, Ministry of Education, Southwest Medical University. Luzhou 646000, China

Received:2016-09-28 Online:2017-04-20 Published:2017-04-24

摘要/Abstract

摘要： 目的:探究A型钾离子(Potassium,K⁺)通道抑制后对舌鳞状细胞癌株(Tca8113)细胞的影响。方法:全细胞膜片钳技术记录和分析A型K⁺通道阻滞剂4-氨基吡啶(4-AP)对Tca8113细胞K⁺电流的影响;MTT检测A型K⁺通道对细胞增殖的作用。结果:在终浓度为2 mmol/L 4-AP作用下,A型K⁺电流的峰值从(267.04±13.84) pA降到(124.81 ±5.24) pA。在一定范围内,不同浓度4-AP能抑制Tca8113细胞在体外的增殖,且抑制程度随药物浓度和作用时间的增加而增强。结论:A型K⁺通道参与Tca8113细胞增殖的过程,且A型K⁺通道阻滞剂能抑制Tca8113细胞的增殖。

Abstract: Objective: To explore the effect of A-type potassium channels on the proliferation of Tca8113 cells. Methods: The whole cell patch clamp technique was used to record and analyze the inhibitory effect of 4-AP on A-type K⁺ current in Tca8113 cells, and MTT tests were used to test the inhibitory effect of 4-AP on cell proliferation in vitro. Results: Under the concentration of 2 mmol/L 4-AP, the peak of A type K⁺ current was decreased from (267.04±13.84)pA to (124.81 ±5.24)pA. The different concentrations of 4-AP could inhibit the proliferation of Tca8113 cells, and the magnitude of inhibitive effect was concentrations and reaction time dependent. Conclusion: The A-type of K⁺ channels play a role in the process of Tca8113 cell proliferation, and the blocker of A-type K⁺ channel can inhibit the proliferation of Tca8113 cells.

Key words: Tongue neoplasms , Squamous cell 4-amino pyridine, Potassium channels , Voltage-gated, Cell proliferation

中图分类号:

R739.8

张晓艳, 程俊, 许华丽, 毛亮, 聂敏海. A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响[J]. 口腔医学研究, 2017, 33(4): 440-443.

ZHANG Xiao-yan, CHENG Jun, XU Hua-li, MAO Liang, NIE Min-hai.. Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells.[J]. Journal of Oral Science Research, 2017, 33(4): 440-443.

参考文献

[1] Shieh CC, Coghlan M, Sullivan JP, et al. Potassium Channels: Molecular Defects, Diseases, and Therapeutic Opportunities [J]. Pharmacology and Experimental Therapeutics, 2000, 52(4)∶557-594
[2] DeCoursey TE, Chandy KG, Gupta S, et al. Voltage-gated K+ channels in human T lymphocytes: a role in mitogenesis [J]. Nature,1984,307(5950)∶465-468
[3] Ryland KE, Hawkins AG, Weisenberger DJ.Promoter Methylation Analysis Reveals That KCNA5 Ion Channel Silencing Supports Ewing Sarcoma CellProliferation [J]. Mol Cancer Res, 2016, 14(1)∶26-34
[4] Rabjerg M,Olivn-Viguera A, Hansen LK,et al.High expression of KCa3.1 in patients with clear cell renal carcinoma predicts high metastatic risk and poor survival [J]. PLoS One, 2015, 10(4)∶e0122992
[5] Cherubini A, Taddei GL, Crociani O, et al. HERG potassium channels are more frequently expresesd in human end 6 metrial cancer as compared to non-cancerous endometrium [J]. Br J Cancer,2000,83(12)∶1722-1729
[6] Siegel RL, Miller KD, Jemal A. Cancer Statistics, 2016 [J]. CA CANCER J CLIN,2016,66(1)∶7-30
[7] Shiboski CH, Schmidt BL, Jordan RC. Tongue and tonsilcarcinoma: Increasing trends in the U.S. Population ages 20-44 years [J]. Cancer,2005,103(9)∶1843-1849
[8] Ouadid-Ahidouch H, Chaussade F, Roudbaraki M, et al. KV1.1 K⁺ channels identification in human breast carcinoma cells: Involvement in cell proliferation [J]. Biochem Biophys Res Commun,2000,278(2)∶272-277
[9] Lew TS, Chang CS, Fang KP, et al.The involvement of K(v)3.4 voltage-gated potassium channel in the growth of an oral squamous cell carcinoma cell line [J]. Oral Pathol Med,2004,33(9)∶543-549
[10] Berzingi S,Newman M, Yu HG. Altering bioelectricity on inhibition of human breast cancer cells [J]. Cancer Bell Int, 2016, 16∶72-76
[11] Jang SH, Choi SY, Ryu PD, et al. Anti-proliferative effect of Kv1.3 blockers in A549 human lung adenocarcinoma in vitro and in vivo [J].Eur J Pharmacol, 2011, 651(1-3)∶26-32
[12] 缪克红,聂敏海,伍宝琴,等.钾离子通道阻滞剂4-AP对人舌鳞癌Tca8113细胞增殖的影响[J].实用口腔医学杂志,2016,32(2)∶230-234
[13] Platoshyn O,Golovina VA,Bailey CL, et al. Sustained membrane depolarization and pulmonary artery smooth muscle cell proliferation [J]. Am J Physiol Cell Physiol,2000,279(5)∶C1540-1549
[14] Kohler R, Degenhardt C , Kuhn M, et al. Expression and function of endothelial Ca²⁺ -activated K+ channels in human mesenteric artery: a single-cell reverse transcriptase- polymerase chain reaction and electrophysiological study in situ [J]. Circ Res,2000,87(6)∶496-503
[15] 岳万远,陈文飞,吴勇,等.醋酸棉酚对人腺样囊性癌ACC-M细胞生长及E-cadherin基因甲基化的影响[J].口腔医学研究,2014,30(3)∶201-204
[16] 陆瑶伽,杨雪超,樊明文.舌鳞状细胞癌中肿瘤干细胞耐药性研究[J].口腔医学研究,2012,28(1)∶1-4

A型钾离子通道对人舌鳞癌Tca8113细胞增殖的影响

Effects of A-type Potassium Ion Channels on the Proliferation of Human Tongue Squamous Cell Carcinoma Tca8113 Cells.

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	胡勤刚, 王玉凤, 韩伟. 肿瘤干细胞在头颈部鳞状细胞癌中的研究进展[J]. 口腔医学研究, 2018, 34(2): 107-111.
[2]	邹海啸, 杨海丽, 赵怡芳, 宋莉. 普萘洛尔对人黑色素瘤A375细胞增殖迁移和血管生成能力的影响及其机制研究[J]. 口腔医学研究, 2018, 34(2): 139-143.
[3]	秦帅华, 李新明, 李文鹿. 修复重建后口腔癌患者生存质量的影响因素分析[J]. 口腔医学研究, 2018, 34(2): 144-147.
[4]	邓轩, 阳芳. MUC1-siRNA对人舌癌Tca细胞迁移、侵袭及PI3K-Akt信号通路的影响[J]. 口腔医学研究, 2018, 34(2): 148-151.
[5]	张厚华, 范华俐. RNA干扰沉默钙蛋白酶1对口腔鳞状细胞癌细胞株HSC3增殖和迁移的影响[J]. 口腔医学研究, 2018, 34(2): 152-156.
[6]	王丽, 李建国. 右美托咪定用于口腔癌前臂皮瓣修复术后保留气管插管患者的镇静研究[J]. 口腔医学研究, 2018, 34(2): 157-160.
[7]	刘玉兰, 孟琳, 唐琪, 布文奂, 黄国帅, 戴义宾, 刘麒麟, 孙宏晨. 抗坏血酸碳点通过促进自噬杀伤舌鳞癌细胞[J]. 口腔医学研究, 2018, 34(1): 35-38.
[8]	周雄明, 那思家, 潘淑婷, 邱嘉旋. 白花丹素对舌鳞状细胞癌细胞的侵袭和迁移的作用及机制研究[J]. 口腔医学研究, 2018, 34(1): 39-43.
[9]	曾光,卫克文. MAP4K3-mTOR信号在甘草查而酮A诱导的鳞癌细胞自噬与凋亡中的作用研究[J]. 口腔医学研究, 2017, 33(12): 1241-1245.
[10]	徐晓芳,贾晓威,梁立中. miR-30d在舌鳞状细胞癌组织中表达及反义miR-30d对细胞增殖、侵袭能力的影响[J]. 口腔医学研究, 2017, 33(12): 1262-1265.
[11]	张红超,刘云青,邵明敬,王翠萍,毕良佳,孟培松. AE-PDT对裸鼠舌鳞癌治疗效果的初步探讨[J]. 口腔医学研究, 2017, 33(12): 1266-1269.
[12]	唐彩金,曾启新,方梅飞,马飞,陶人川,Niyukty Arjal. 消退素D1治疗实验性大鼠牙周炎的研究[J]. 口腔医学研究, 2017, 33(12): 1270-1273.
[13]	吴昌敬,王蔚璐,甄恩明,邵军. YAP1小干扰RNA对舌鳞状细胞癌生物学特性的影响[J]. 口腔医学研究, 2017, 33(12): 1278-1281.
[14]	邬利波,潘淑婷,邱嘉旋. 口腔鳞癌新辅助化疗前后多药耐药相关蛋白的表达及临床疗效分析[J]. 口腔医学研究, 2017, 33(12): 1298-1301.
[15]	王丽萍, 郭雪琪, 闫勇勇, 査骏, 陈伟鸿, 魏永祥, 朱昕欣, 管红兵, 葛林虎. miR-146a在口腔鳞癌组织及细胞中的表达及功能研究[J]. 口腔医学研究, 2017, 33(11): 1204-1208.