Effects of Daidzein on Bone Rebuiling of Alveolar Fossa after Tooth Extraction in Osteoporotic-model Rats.

doi:10.13701/j.cnki.kqyxyj.2017.03.006

Abstract

Abstract: Objective: To explore the effect of Daidzein (Da) on the alveolar bone rebuilding after tooth extraction in osteoporotic-model rats. Methods: Twenty-four 3-months-old female SD rats were randomly divided into four groups: sham group (Sham), estrogen group (EG), ovariectomized group (Ovx), ovariectomized and Daidzein group (Da). Bilateral maxillary first molars of all rats were extracted at the 7th day post ovarian surgery. The values of serum calcium, phosphorus and alkaline phosphatase (ALP) were recorded at the 12th week. Meanwhile, bone tissue sections of the left side of alveolar bone were made to observe bone rebuilding. Results: The value of serum calcium(2.49±0.02) and ALP(5.31±0.5) of ovariectomized rats decreased significantly(P<0.01), Daidzein could significantly improve the value of calcium(2.27±0.05)and the activity of ALP(4.49±0.38) in serum of ovariectomized rats(P< 0.01).Morphological observation in alveolar bone tissue around the tooth fossa showed that the function of bone preservation of Daidzein was similar to estrogen, and was relatively similar to that of Sham group. Conclusion: Daidzein can remarkably improve the value of calcium and ALP in serum, reduce resorption of alveolar bone around the tooth fossa, and rebuild the bone morphology after extraction in osteoporotic-model rats.

Key words: Daidzein , Osteoporosis , Trace elements, Tooth extraction, Bone rebuilding

CLC Number:

R782.1

ZHANG Yu, LIN JIU, GAO Wei, HU Yi, GAO Yi, XIONG Wen-bi, PAN Jian.. Effects of Daidzein on Bone Rebuiling of Alveolar Fossa after Tooth Extraction in Osteoporotic-model Rats.[J]. Journal of Oral Science Research, 2017, 33(3): 258-261.

References

[1] Ames MS, Hong S, Lee HR, et al. Estrogen deficiency increases variability of tissue mineral density of alveolar bone surrounding teeth [J]. Archives of Oral Biology, 2010, 55(8)∶599-605
[2] 骆凯,闫福华,陈凌,等.绝经后骨质疏松大鼠实验性牙周炎动物模型研究[J].口腔医学研究,2006,22(2)∶130-132
[3] Chen H, Liu N, Xu X, et al. Smoking, radiotherapy, diabetes and osteoporosis as risk factors for dental implant failure: a meta-analysis [J]. PLoS One, 2013, 8(8)∶e71955
[4] 李儒煌, 蔡志刚. 二膦酸盐相关性颌骨坏死研究进展[J].口腔医学研究,2012,28(11)∶1197-1199
[5] Nakajima Y, Akaogi K, Suzuki T, et al. Estrogen regulates tumor growth through a nonclassical pathway that includes the transcription factors ERα and KLF5 [J]. Sci. Signal., 2011, 4(168)∶ra22-ra22
[6] Wei P, Liu M, Chen Y, et al. Systematic review of soy isoflavone supplements on osteoporosis in women [J]. Asian Pacific journal of tropical medicine, 2012, 5(3)∶243-248
[7] 包蕾.大豆苷原对骨形成的量效作用及 ERs/PPARγ的调节机制[D].复旦大学,2011
[8] Lelovas PP, Xanthos TT, Thoma SE, et al. The laboratory rat as an animal model for osteoporosis research [J]. Comparative medicine, 2008, 58(5)∶424-430
[9] 张祎,金邦荃,汤丹,等.大豆甙元对大鼠骨形态学及生物力学影响[J].中国公共卫生,2008,24(5)∶540-542
[10] ArjmandiBH, AlekelL, HollisBW, et al. Dietary soybean protein prevents bone loss in an ovariectomized rat model of osteoporosis [J]. JNutr,1996,126(1)∶161-167
[11] 包蕾,邹世恩,张绍芬.雌激素受体和过氧化物酶体增殖物激活受体γ介导大豆苷原对骨形成的量效作用[J].中西医结合学报,2011,9(2)∶165-172
[12] Du Z, Xiao Y, Hashimi S, et al. The effects of implant topography on osseointegration under estrogen deficiency induced osteoporotic conditions: Histomorphometric, transcriptional and ultrastructuralanalysis [J]. ActaBiomaterialia, 2016, 42(3)∶351-363
[13] Wang Q, Ge X, Tian X, et al. Soy isoflavone: The multipurpose phytochemical (Review) [J]. Biomedical Reports, 2013, 1(5)∶697-701
[14] Imai Y, Kondoh S, Kouzmenko A, et al. Minireview: osteoprotective action of estrogens is mediated byosteoclastic estrogen receptor-α [J]. Molecular Endocrinology, 2010, 24(5)∶877-885
[15] 高美,金邦荃,张祎,等.大豆甙元对去卵巢大鼠雌二醇,维生素D和骨矿沉积的影响[J].中国老年学杂志,2009(12)∶1480-1482