Journal of Oral Science Research

Journal of Oral Science Research ›› 2017, Vol. 33 ›› Issue (7): 712-716.DOI: 10.13701/j.cnki.kqyxyj.2017.07.006

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Biological Evaluation of Three-dimensional Printed β-TCP Scaffold for Jaw Reconstruction.

CAO Shuai-shuai1, ZHOU Miao1, MIRANDA Pedro2, CHE Yue-juan3, CHEN Xiao-ming4, LI Shu-yi1, GENG Yuan-ming5, YANG Xiao-bin1.   

  1. 1.Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China;
    2. Departamento de Ingeniería Mecánica, Energética y de los Materiales, Universidad de Extremadura, Spain 999023;
    3. Department of Anesthesiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China;
    4. Biomedical Engineering Department, Guangzhou Medical University, Guangzhou 511436, China;
    5. Department of Stomatology, Zhujiang Hospital, Southern Medical University Guangzhou 510280, China.
  • Received:2016-12-15 Online:2017-07-20 Published:2017-07-27

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Abstract: Objective: To explore the biological characteristics and in vivo osteogenesis of β-tricalcium phosphate (β-TCP) scaffold fabricated by three-dimensional printing (3DP). Methods: β-TCP scaffolds were fabricated by robocasting. MC3T3-E1 cells were seeded onto the scaffold, then scanning electron microscope (SEM) was used to characterize the surface morphology and cells’ adhesion. The proliferation and differentiation capability of the cells were assessed by cell counting kit-8 (CCK-8) and alkaline phosphatase activities (ALP). The scaffolds incorporated with recombinant human bone morphogenetic protein-2 (rhBMP-2) were implanted into the rats, while foaming-made β-TCP scaffolds were used as control. The bone formation was observed by histological examination in 6 weeks after operation. Results: The scaffold possessed a well-defined porous stereo-structure. The cell proliferation and differentiation capacity of the 3DP scaffold were significantly higher than those in control group (P<0.05). Histological examination showed higher new bone formation of the 3DP β-TCP than foaming-made scaffold incorporated with rhBMP-2 (P<0.05). Conclusion: 3DP β-TCP scaffold showed an excellent biocompatibility and ectopic osteogenesis.

Key words: Three-dimensional printing, Robocasting , β-TCP rh, BMP-2 , Bone repair

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