Abstract: Objective: To explore the effect of hBMP-2 modified beta TCP/collagen I composite on the proliferation of MC3T3-E1 cells. Methods: Nano beta TCP/collagen I solution composite materials were modified by plasmid DNA containing hBMP-2 as the target gene, then the MC3T3-E1 cell line and nano composite materials in vitro culture system were established. Cells were divided into stent group and plate group, scanning electron microscope (SEM) and optical microscopy were used to observe the cell morphology, while cell proliferation and cycle were detected by Alamar Blue and MuseTM Cell analyzer assays, respectively. Real-time fluorescence quantitative PCR were applied to measure cell proliferation genes Cdk2 and Cdk4. Results: Cell adhesion, proliferation and distribution of the stent group were higher than those of the plate group (P<0.05). Conclusion: Compared to the plasmid DNA containing hBMP-2 only, hBMP-2 modified beta TCP/collagen I composite was better at stimulating preosteoblast adhesion, proliferation and metabolism. It might be used as bone repair or tissue engineering materials.

Key words: BMP-2, Beta three calcium phosphate/collagen, Proliferation