Journal of Oral Science Research

Journal of Oral Science Research ›› 2017, Vol. 33 ›› Issue (10): 1023-1026.DOI: 10.13701/j.cnki.kqyxyj.2017.10.001

• Orignal Article •     Next Articles

Study on Immune Efficacy of Self-assembling Anti-caries Nanoparticle Vaccine

YE Qian-lin1, LI ZHEN2, CAO Xi-xi1, LIU Chang1, FAN Ming-wen2*   

  1. 1.The State Key Laboratory Breeding of Basic Science of Stomatology (Hubei- Most) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
    2.Department of Anesthesiology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China.
  • Received:2017-08-16 Online:2017-10-20 Published:2017-10-24

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Abstract: Objective: To study the efficacy of self-assembling anti-caries nanoparticle vaccine Glu-FTH and Glu + Poly (I:C) on inducing protective humoral immune response and mucosal immune response in mice. Methods: The mice were separated into 6 groups randomly. Glu-FTH, Glu, Glu-FTH+Poly (I:C), Glu+Poly (I:C), FTH and PBS were respectively delivered into mice by intranasal administration. The levels of specific antibodies in serum and saliva were tested by enzyme-linked immunosorbent assay (ELISA). Results: The levels of serum anti-Glu IgG in Glu-FTH, Glu, Glu-FTH+Poly (I:C), Glu+Poly(I:C) group increased. The levels of salivary anti-Glu sIgA in Glu+Poly(I:C) and Glu also increased. Conclusion: Anti-caries vaccine Glu-FTH and the synergy of Poly (I:C) and Glu can induce specific immune response.

Key words: Caries, Self-assembling nanoparticle vaccine, Poly(I:C), Adjuvant, Mucosal immune

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