Effect of Pretreatment with EDTA and EDC on Bond Strength of Two-step Self-etching Adhesive

doi:10.13701/j.cnki.kqyxyj.2020.03.008

Abstract

Abstract: Objective: To study the effect of pretreatment with EDTA and EDC on the bond strength of two-step self-etching adhesive. Methods: 56 third molars were randomly divided into 8 groups according the dentin surface treatment and whether or not aging (n=7), group A and A1: Clearfil SE Bond; group B and B1:17% EDTA+Clearfil SE Bond; group C and C1:0.3 mol/L EDC+Clearfil SE Bond; group D and D1:17% EDTA+ 0.3 mol/L EDC+Clearfil SE Bond. Specimens of A1, B1, C1 and D1 were conducted aging test. 5 specimens were randomly selected from each group to test the bond strength and analyze the failure mode. The longitudinal profile morphology of the remaining 2 specimens in each group was observed. Results: Group D had the highest immediate bond strength, and the difference with group A was statistically significant (P<0.05). The difference between group C and C1, group D and D1 was no statistically significant (P>0.05). Mixed fracture was the main failure mode, and the difference was no statistically significant. Resin tags in group B, B1, D and D1 were more and longer than other groups, and no cracks were found in the mixing layer of group C1 and D1. Conclusion: Pretreatment with 17% EDTA+0.3 mol/L EDC can improve immediate dentin bond strength of two-step self-etching adhesive and the adhesion is durable.

Key words: ethylene diamine tetraacetic acid, carbodiimide, dentin, bond strength

DENG Ting, LIU Min, LIU Wen, WANG Wen, XIE Cuiliu. Effect of Pretreatment with EDTA and EDC on Bond Strength of Two-step Self-etching Adhesive[J]. Journal of Oral Science Research, 2020, 36(3): 221-225.

References

[1] Schuldt C, Birlbauer S, Pitchika V, et al. Shear bond strength and microleakage of a new self-etching/self-adhesive pit and fissure sealant[J]. J Adhes Dent, 2015, 17(6): 491-497.
[2] Manuja N, Nagpal R, Chaudhary S. Bonding efficacy of 1-step self-etch adhesives: effect of additional enamel etching and hydrophobic layer application[J]. J Dent Child, 2012, 79(1): 3-8.
[3] Breschi L, Maravic T, Cunha SR, et al. Dentin bonding systems: From dentin collagen structure to bond preservation and clinical applications[J]. Dent Mater, 2018, 34(1): 78-96.
[4] Abunawareg M, Abuelenain DA, Elkassas D, et al. Role of dentin cross-linking agents in optimizing dentin bond durability[J]. Int J Adhes Adhes, 2017, 78: 83-88.
[5] Frassetto A, Breschi L, Turco G, et al. Mechanisms of degradation of the hybrid layer in adhesive dentistry and therapeutic agents to improve bond durability--A literature review[J]. Dent Mater, 2016, 32(2): 41-53.
[6] Mazzoni A, Apolonio FM, Saboia VP, et al. Carbodiimide inactivation of MMPs and effect on dentin bonding[J]. J Dent Res, 2014, 93(3): 263 -268.
[7] Scheffel DLS, Bianchi L, Soares DG, et al. Transdentinal cytotoxicity of carbodiimide (EDC) and glutaraldehyde on odontoblast-like cells[J]. Oper Dent, 2015, 40(1): 44-54.
[8] 王丽霞, 赵寰, 靳淑凤, 等. EDC交联离子化胶原支架材料的生物学表征[J]. 口腔医学研究, 2015, 31(4): 343-348.
[9] Tang L, Zhang Y, Liu Y, et al. Influence of EDC on dentin-resin shear bond strength and demineralized dentin thermal properties[J]. Materials (Basel), 2016, 9(11). pii: E920.
[10] Scheffel D, Delgado C, Soares DG, et al. Increased durability of resin-dentin bonds following cross-linking treatment[J]. Oper Dent, 2015, 40(5): 533-539.
[11] Tezvergil-Mutluay A, Mutluay MM, Agee KA, et al. Carbodiimide cross-linking inactivates soluble and matrix-bound MMPs, in vitro[J]. J Dent Res, 2012, 91(2): 192-196.
[12] Kim DS, Park SH, Choi GW, et al. Effect of EDTA treatment on the hybrid layer durability in total-etch dentin adhesives[J]. Dent Mater J, 2011, 30(5): 717-722.
[13] Singh S, Nagpal R, Tyagi SP, et al. Effect of EDTA Conditioning and Carbodiimide Pretreatment on the Bonding Performance of All-in-One Self-Etch Adhesives[J]. Int J Dent, 2015, 2015: 141890.
[14] 田福聪, 王晓燕, 高学军. EDTA表面处理对牙本质粘接强度的影响[J]. 实用口腔医学杂志, 2013, 29(4): 550-552.
[15] Luque-Martinez IV, Munoz MA, Hass V, et al. EDTA conditioning increases the long-term microtensile bond strength to sclerotic dentin mediated by self-etch adhesives[J]. J Adhes Dent, 2018, 20(5): 397-403.
[16] Gandolfi MG, Taddei P, Pondrelli A, et al. Demineralization, collagen modification and remineralization degree of human dentin after EDTA and citric acid treatments[J]. Materials (Basel), 2018, 12(1): 1-5.
[17] Tezvergil MA, Agee KA, Hoshika T, et al. The inhibitory effect of polyvinylphosphonic acid on functional matrix metalloproteinase activities in human demineralized dentin[J]. Acta Biomater, 2010, 6(10): 4136-4142.
[18] Mazzoni A, Angeloni V, Apolonio FM, et al. Effect of carbodiimide (EDC) on the bond stability of etch-and-rinse adhesive systems[J]. Dent Mater, 2013, 29(10): 1040-1047.
[19] Shafiei F, Yousefipour B, Mohammadi-Bassir M. Effect of carbodiimide on bonding durability of adhesive-cemented fiber posts in root canals[J]. Oper Dent, 2016, 41(4): 432-440.
[20] 唐琳,张一,李皓,等.不同浓度的碳二亚胺乙醇溶液处理对牙本质自酸蚀粘接剂粘接强度的影响[J].北京大学学报(医学版),2017,49(6):1055-1059.
[21] 张一,刘玉华,周永胜,等.碳二亚胺-乙醇溶液表面处理对牙本质微拉伸粘结强度的影响[J].北京大学学报,2015,47(5): 825-828.