Effect of Diode, Er, and Nd:YAG Laser Irradiation on Microstructure of Microarc Oxidation Titanium Disc and Osteogenesis Activity of Periodontal Ligament Stem Cells

doi:10.13701/j.cnki.kqyxyj.2022.11.011

Abstract

Abstract: Objective: To study the effect of diode, Er, and Nd:YAG laser irradiation on the microstructure of microarc oxidation (MAO) titanium disks and osteogenesis activity of periodontal ligament stem cells. Methods: The diode, Er, and Nd:YAG laser were applied to irradiate the MAO titanium disks, after which scanning electron microscope (SEM) was used to observe the morphology of discs and the seeded human periodontal ligament stem cells (hPDLSCs). Crystal phase of MAO titanium disc was analyzed by X-ray diffraction (XRD). The proliferation of hPDLSCs was determined by CCK-8. The expressions of Runx2, ALP, OCN, and OSX gene in hPDLSCs were detected by RT-qPCR. Results: SEM images and XRD spectrum revealed that diode, Er, and Nd:YAG laser could alter the topography and crystal phases of MAO titanium discs. The expressions of Runx2 and ALP genes of hPDLSCs on the surface of MAO titanium disks irradiated by Nd:YAG laser were significantly increased (P<0.001), but the proliferation was reduced (P<0.05). The expression of ALP gene of cells on the surface of disks irradiated by Er laser was increased (P<0.005); however, the proliferation and osteoblastic gene expression of hPDLSCs on the disks irradiated by Diode laser were decreased. Conclusion: Irradiation by Er and Nd:YAG laser could effectively promote the osteogenic differentiation of hPDLSCs on the MAO titanium disks, however, irradiation by diode laser deteriorated the osteogenic differentiation and proliferation of cells.

Key words: Diode laser, Er laser, Nd:YAG laser, microarc oxidation titanium, human periodontal ligament stem cells

HUANG Wenxia, ZUO Qiliang, ZHANG Qiufang, NIE Jing, HAN Yangping, XU Minyan, ZHAN Xuan. Effect of Diode, Er, and Nd:YAG Laser Irradiation on Microstructure of Microarc Oxidation Titanium Disc and Osteogenesis Activity of Periodontal Ligament Stem Cells[J]. Journal of Oral Science Research, 2022, 38(11): 1052-1057.

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