Advances of N6-methyladenine RNA Methylation in Oral Development and Diseases

doi:10.13701/j.cnki.kqyxyj.2023.09.003

Abstract

Abstract: Epigenetic regulation refers to the regulation of gene expression through epigenetic modification without alteration of genetic material sequence. N6-methyladenosine (m6A) RNA methylation is one of regulation approaches. The expression level of target RNA is modified by methyltransferase, demethylase, and recognition protein. This article reviews the role and mechanism of m6A and its regulatory molecules in oral development and regeneration, oral inflammatory diseases, and oral tumor diseases.

Key words: epigenetics, m6A modification, m6A regulators, target RNA

HUANG Jing, ZHOU Yi. Advances of N6-methyladenine RNA Methylation in Oral Development and Diseases[J]. Journal of Oral Science Research, 2023, 39(9): 775-779.

References

[1] Fu Y, Dominissini D, Rechavi G, et al. Gene expression regulation mediated through reversible m6A RNA methylation [J]. Nat Rev Genet,2014,15(5):293-306
[2] Roundtree IA, Evans ME, Pan T, et al. Dynamic RNA modifications in gene expression regulation [J]. Cell, 2017, 169(7):1187-1200.
[3] Meyer KD, Jaffrey SR. Rethinking m6A readers, writers, and erasers [J]. Annu Rev Cell Dev Biol, 2017, 33:319-342.
[4] Ping XL, Sun BF, Wang L, et al. Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase [J]. Cell Res, 2014, 24(2):177-189.
[5] Zhang S, Zhao BS, Zhou A, et al. m6A demethylase ALKBH5 maintains tumorigenicity of glioblastoma stem-like cells by sustaining FOXM1 expression and cell proliferation program [J]. Cancer Cell, 2017, 31(4):591-606.
[6] Huang H, Weng H, Sun W, et al. Recognition of RNA N⁶-methyladenosine by IGF2BP proteins enhances mRNA stability and translation [J]. Nat Cell Biol, 2018, 20(3):285-295.
[7] 金岩.口腔颌面发育生物学与再生医学[M].第2版.北京:人民卫生出版社,2020.1-22.
[8] Sheng R, Wang Y, Wu Y, et al. METTL3-Mediated m6A mRNA methylation modulates tooth root formation by affecting NFIC translation [J]. J Bone Miner Res, 2021, 36(2):412-423.
[9] Tian C, Chai J, Liu W, et al. Role of the demethylase AlkB homolog H5 in the promotion of dentinogenesis [J]. Front Physiol, 2022, 13:923185.
[10] Sun Q, Zhao T, Li B, et al. FTO/RUNX2 signaling axis promotes cementoblast differentiation under normal and inflammatory condition [J]. Biochim Biophys Acta Mol Cell Res, 2022, 1869(12):119358.
[11] Luo H, Liu W, Zhang Y, et al. METTL3-mediated m6A modification regulates cell cycle progression of dental pulp stem cells [J]. Stem Cell Res Ther, 2021, 12(1):159.
[12] Cai W, Ji Y, Han L, et al. METTL3-dependent glycolysis regulates dental pulp stem cell differentiation [J]. J Dent Res, 2022, 101(5):580-589.
[13] Jiang W, Zhu P, Huang F, et al. The RNA methyltransferase METTL3 promotes endothelial progenitor cell angiogenesis in mandibular distraction osteogenesis via the PI3K/AKT pathway [J]. Front Cell Dev Biol, 2021, 9:720925.
[14] Xiong Q, Liu C, Zheng X, et al. METTL3-mediated m6A RNA methylation regulates dorsal lingual epithelium homeostasis [J]. Int J Oral Sci, 2022, 14(1):26.
[15] Caetano AJ, Yianni V, Volponi A, et al. Defining human mesenchymal and epithelial heterogeneity in response to oral inflammatory disease [J]. Elife, 2021, 10:e62810.
[16] Feng Z, Li Q, Meng R, et al. METTL3 regulates alternative splicing of MyD88 upon the lipopolysaccharide-induced inflammatory response in human dental pulp cells [J]. J Cell Mol Med, 2018, 22(5):2558-2568.
[17] Lin W, Xu H, Wu Y, et al. In silico genome-wide identification of m6A-associated SNPs as potential functional variants for periodontitis [J]. J Cell Physiol, 2020, 235(2): 900-908.
[18] Zhang X, Zhang S, Yan X, et al. m6A regulator-mediated RNA methylation modification patterns are involved in immune microenvironment regulation of periodontitis [J]. J Cell Mol Med, 2021, 25(7):3634-3645.
[19] Wu Z, Lin W, Yuan Q, et al. A genome-wide association analysis:m6A-SNP related to the onset of oral ulcers [J]. Front Immunol, 2022, 13:931408.
[20] 胡勤刚,泥艳红,王雨晗.代谢组学在口腔鳞状细胞癌早期诊断及精准诊疗中的研究进展[J].口腔医学研究,2022,38(3):207-211.
[21] Wu X, Tang J, Cheng B. Oral squamous cell carcinoma gene patterns connected with RNA methylation for prognostic prediction [J]. Oral Dis, 2022.
[22] Xu L, Yu C, Du XJ. Prognostic evaluation for oral squamous cell carcinoma:A novel method based on m6A methylation regulators [J]. Curr Med Sci, 2022, 42(4):841-846.
[23] Shan L, Lu Y, Song Y, et al. Identification of nine m6A-related long noncoding RNAs as prognostic signatures associated with oxidative stress in oral cancer based on data from the cancer genome atlas [J]. Oxid Med Cell Longev, 2022, 2022:9529814.
[24] Yang Q, Cheng C, Zhu R, et al. A N6-methyladenosine-related long noncoding RNAs model for predicting prognosis in oral squamous cell carcinoma: Association with immune cell infiltration and tumor metastasis [J]. Oral Oncol, 2022, 127:105771.
[25] Wang X, Wu J, Zhang L, et al. Methylated RNA immunoprecipitation sequencing reveals the m6A landscape in oral squamous cell carcinoma [J]. J Immunol Res, 2022, 2022:7277583.
[26] Wu S, Makeudom A, Sun X, et al. Overexpression of methyltransferase-like 3 and 14 in oral squamous cell carcinoma [J]. J Oral Pathol Med, 2022, 51(2):134-145.
[27] Ai Y, Liu S, Luo H, et al. METTL3 intensifies the progress of oral squamous cell carcinoma via modulating the m6A amount of PRMT5 and PD-L1 [J]. J Immunol Res, 2021, 2021:6149558.
[28] Liu L, Wu Y, Li Q, et al. METTL3 promotes tumorigenesis and metastasis through BMI1 m6A methylation in oral squamous cell carcinoma [J]. Mol Ther, 2020, 28(10):2177-2190.
[29] Zhao W, Cui Y, Liu L, et al. METTL3 facilitates oral squamous cell carcinoma tumorigenesis by enhancing c-Myc stability via YTHDF1-mediated m⁶A modification [J]. Mol Ther Nucleic Acids, 2020, 20:1-12.
[30] Xu T, Zhang W, Chai L, et al. Methyltransferase-like 3-induced N6-methyladenosine upregulation promotes oral squamous cell carcinoma by through p38 [J]. Oral Dis, 2023, 29(2):639-648.
[31] Wang F, Zhu Y, Cai H, et al. N6-methyladenosine methyltransferase METTL14-mediated autophagy in malignant development of oral squamous cell carcinoma [J]. Front Oncol, 2021, 11:738406.
[32] Li J, Momen-Heravi F, Wu X, et al. Mechanism of METTL14 and m6A modification of lncRNA MALAT1 in the proliferation of oral squamous cell carcinoma cells [J]. Oral Dis, 2022, 29(5):2012-2026.
[33] Li X, Chen W, Gao Y, et al. Fat mass and obesity-associated protein regulates arecoline-exposed oral cancer immune response through programmed cell death-ligand 1 [J]. Cancer Sci, 2022, 113(9):2962-2973.
[34] Li DQ, Huang CC, Zhang G, et al. FTO demethylates YAP mRNA promoting oral squamous cell carcinoma tumorigenesis [J]. Neoplasma, 2022, 69(1):71-79.
[35] Wang F, Liao Y, Zhang M, et al. N6-methyladenosine demethyltransferase FTO-mediated autophagy in malignant development of oral squamous cell carcinoma [J]. Oncogene, 2021, 40(22):3885-3898.
[36] Huang GZ, Wu QQ, Zheng ZN, et al. M6A-related bioinformatics analysis reveals that HNRNPC facilitates progression of OSCC via EMT [J]. Aging(Albany NY), 2020, 12(12):11667-11684.
[37] Zhu F, Yang T, Yao M, et al. HNRNPA2B1, as a m6A reader, promotes tumorigenesis and metastasis of oral squamous cell carcinoma [J]. Front Oncol, 2021, 11:716921.
[38] Xu K, Dai X, Wu J, et al. N⁶-methyladenosine(m6A)reader IGF2BP2 stabilizes HK2 stability to accelerate the Warburg effect of oral squamous cell carcinoma progression [J]. J Cancer Res Clin Oncol, 2022, 148(12):3375-3384.
[39] Zhao W, Liu J, Wu J, et al. High-throughput microarray reveals the epitranscriptome-wide landscape of m6A-modified circRNA in oral squamous cell carcinoma [J]. BMC Genomics, 2022, 23(1):1-13.
[40] Cui Y, Liu J, Liu L, et al. m6A-modified circFOXK2 targets GLUT1 to accelerate oral squamous cell carcinoma aerobic glycolysis [J]. Cancer Gene Ther, 2023, 30(1):163-171.
[41] Gong J, Wang C, Zhang F, et al. Effects of allocryptopine on the proliferation and epithelial-mesenchymal transition of oral squamous cell carcinoma through m6A mediated hedgehog signaling pathway [J]. J Environ Pathol Toxicol Oncol, 2022, 41(2):15-24.
[42] Luo R, Xie L, Lin Y, et al. Oxymatrine suppresses oral squamous cell carcinoma progression by suppressing CXC chemokine receptor 4 in an m6A modification decrease dependent manner [J]. Oncol Rep, 2022, 48(4):1-10.
[43] Chen J, Li S, Huang Z, et al. METTL3 suppresses anlotinib sensitivity by regulating m6A modification of FGFR3 in oral squamous cell carcinoma [J]. Cancer Cell Int, 2022, 22(1):295.
[44] Niu X, Xu J, Liu J, et al. Landscape of N⁶-methyladenosine modification patterns in human ameloblastoma [J]. Front Oncol, 2020, 10:556497.