Effect of co-culture of BMP-2 and bFGF Transfected Rat Bone Marrow Stem Cells and Germ Cells on Tooth Gene.

Abstract

Abstract: Objective: To observe the bone morphogenetic protein -2 (BMP-2) and (or) basic fibroblast growth factor (bFGF) transfected rat bone marrow stem cells (BMSCs) and germ mixed culture cells in vitro effects on dentin matrix protein 1 (DMP1), ameloblastin (AMBN), Ⅰ collagen (collagen-Ⅰ), homeobox gene 1 (DLX1)'s. Methods: From gene expression and protein levels by real-time quantitative PCR and Western blot to determine the level of BMP-2 and (or) the impact of bFGF on four genes. Results: The control group, bFGF group, BMP-2 group and bFGF / BMP-2 group among the four groups, AMBN, COLLAGEN-Ⅰ, DLX1, DMP1 genes were significantly different (P<0.05) in the expression of mRNA and protein levels. Conclusion: BMP2 and bFGF have synergistic interaction, BMP2 / bFGF / BMSCs / germ cells can be constructed as seed cells for tissue engineering of teeth.

Key words: Bone morphogenetic protein-2 , Basic fibroblast growth factor, Dentin matrix protein 1, Ameloblastin Ⅰ collagen , Homeobox gene 1

CLC Number:

Q132

WU Wei-wei, HU Yang, HE Hui-yu, et al. Effect of co-culture of BMP-2 and bFGF Transfected Rat Bone Marrow Stem Cells and Germ Cells on Tooth Gene.[J]. Journal of Oral Science Research, 2015, 31(1): 18-22.

References

[1] Nakashima M. Bone morphogenetic proteins in dentin regeneration for potential use in endodontic therapy [J]. Cytokine Growth Factor Rev. 2005,16(3)∶369-76
[2] 程敏,刘晓红,程琳,等.碱性成纤维细胞生长因子在牙发育各阶段中的表达及意义[J].吉林大学学报,2009,35(5)∶829-831
[3] 于金华,金岩,史俊南,等.大鼠牙胚细胞团块培养法构建组织工程化牙齿[J].口腔医学,2006,26(1)∶10-12
[4] 郭滨,邹敬才,秦瑞峰,等. 注射性组织工程牙胚的构建及成牙能力研究[J].现代生物医学进展,2007,7(12)∶1776-1778
[5] Duailibi SE,Duailibi MT, Zhang W,et al. Bioengineered Dental Tissues Grown in the Rat Jaw [J]. J Dent Res,2008,87(8)∶745-750
[6] Li H,Marijanovic I,Kronenberg MS,et al. Expression and Function of Dlx Genes in the Osteoblast Lineage [J]. Dev Biol,2008,316(2)∶458-470
[7] Bai Yan,Li Peipei,Yin Guangfu,et al. BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells [J]. Biotechnol Lett,2013,35∶301-308
[8] Wang L, Huang Y, Pan K,et al. Osteogenic Responses to Different Concentrations/Ratios of BMP-2 and bFGF in Bone Formation [J]. Annals of Biomedical Engineering,2010,38(1)∶77-87
[9] 巴娇娇,何惠宇,胡杨,等.牙胚细胞体外培养过程中成牙基因的表达[J].中国组织工程研究,2014,18(2)∶193-198
[10] 王慧,惠艳,刘涛,等.实时荧光定量聚合酶链反应检测人类型疱疹病毒载量方法的建立及其诊断应用[J].2010,28(7)∶413-417
[11] 韩祥祯,何惠宇,胡杨,等.重组慢病毒载体转染羊骨髓间充质干细胞及成骨基因表达量的变化[J]. 中国组织工程研究,2014,18(6)∶821-828
[12] Radhakrishnan VM1,Putnam CW,Qi W,et al. P53 suppresses expression of the 14-3-3gamma oncogene[J]. BMC Cancer,2011,11∶378
[13] 赵征,刘洪臣.组织工程牙再生发育的研究进展[J].中华老年口腔医学杂志,2009 ,7(4)∶248-252
[14] Hung CN,Mar K,Chang HC,et al. A comparison between adipose tissue and dental pulp as sources of MSCs for tooth regeneration [J]. Biomaterials, 2011, 32(29)∶6995-7005
[15] 张文涛,刘建华,王慧明,等.重组人 BMP-2 修饰的β磷酸三钙/胶原材料制备及其诱导成牙性能的初步研究[J].中国修复重建外科杂志,2011,25(2)∶149-154
[16] Miranda SC,Silva GA,Hell RC ,et al.Three-dimensional culture of rat BMMSCs in a porous chitosan-gelatin scaffold: A promising association for bone tissue engineering in oral reconstruction [J]. Archives of oral biology,2011,56(1)∶1-15
[17] Hanada K,Dennis JE,Caplan AI. Stimulatory Effects of Basic Fibroblast Growth Factor and Bone Morphogenetic Protein-2 on Osteogenic Differentiation of Rat Bone Marrow-Derived Mesenchymal Stem Cells [J]. J Bone Miner Res,1997,12(10)∶1606-14
[18] Yang X,van der Kraan PM,van den Dolder J,et al. STRO-1 Selected Rat Dental Pulp Stem Cells Transfected with Adenoviral-Mediated Human Bone Morphogenetic Protein 2 Gene Show Enhanced Odontogenic Differentiation [J]. Tissue Engineering,2007,13(11)∶2803-2812
[19] Bento FM1,Takeshita D,Sacramento CB,et al. Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells [J]. BMC Biotechnology,2004,4∶29