Experimental Study on Acellular Bovine Pericardium Combined with BMP-2 Transfected BMSCs in Guided Bone Regeneration in Vitro.

doi:10.13701/j.cnki.kqyxyj.2016.07.008

Abstract

Abstract: Objective: To study the feasibility of naturally derived acellular bovine pericardium combined with BMP-2 gene transfected BMSCs in guided bone tissue regeneration in vitro. Methods: BMP-2 transfected BMSCs were seeded on the natural acellular bovine pericardium. The cell growth and proliferation on acellular bovine pericardium were assessed by SEM, the cytotoxicity of membrane on transfected cells was assessed by MTT test, the BMP-2 content was measured by ELISA, cell adhesion capacity was detected by crystal violet staining, and osteogenic properties was evaluated by ALP activity. Results: SEM showed that the BMP-2 gene transfected BMSCs could adhere to the acellular bovine pericardium and proliferate well. There was no significant difference in the survival rate of cells in each group. The cell adhesion capacity and the ability of BMP-2 transfected BMSCs transforming into osteoblasts were enhanced by the acellular bovine pericardium. Conclusion: Natural acellular bovine pericardium had well biological and cellular compatibility, which might be used as a good natural GBR membrane material.

Key words: Natural acellular bovine pericardium, BMP-2 gene transfected , BMSCs, GBR , In vitro study

CLC Number:

R318.08

BAI Ming-hai, ZHANG Ting-ting, WU Han-jiang, ZHAO Zhi-he, HE Zheng-quan, LIU Xing-yu.. Experimental Study on Acellular Bovine Pericardium Combined with BMP-2 Transfected BMSCs in Guided Bone Regeneration in Vitro.[J]. Journal of Oral Science Research, 2016, 32(7): 696-698.

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