Effects of Doxorubicin-loaded Mesoporous Silica Nanoparticles on Proliferation and Apoptosis of Human Tongue Cancer Tca-8113 Cells.

doi:10.13701/j.cnki.kqyxyj.2017.03.004

Abstract

Abstract: Objective: To establish a drug delivery system based on mesoporous silica nanoparticles (MSNs) for its potential role in promoting the anticancer efficacy of doxorubicin (DOX) on Tca-8113 cells. Methods: MSNs were characterized by transmission electron microscope (TEM) and N2 adsorption/desorption method. The drug release profiles of DOX from MSNs were monitored by UV-Vis. The anticancer effect of MSNs@DOX was demonstrated by MTT assay, Hoechst 33342 staining, and flow cytometry. The related mechanism was explored by Western blot. Results: The particle size of MSNs was about 30 nm and its average pore size, surface area and pore volume were 19.59682 nm, 193.4296 m²/g and 0.947625 cm³/g, respectively. MSNs displayed good cytocompatibility. MSNs@DOX inhibited the proliferation of Tca-8113 cells, induced apoptosis remarkably and arrested cell cycle at G0/G1 checkpoints. Western blot revealed remarkable up-regulated expression of Bax, p53 and caspase-3 and down-regulated expression of Bcl-2. Conclusion: Such a doxorubicin delivery strategy might be promising in tongue cancer therapy.

Key words: Doxorubicin , Mesoporous silica nanoparticles , Tongue cancer, Tca-8113 cells

CLC Number:

R739.8

LI Hai-qin, FAN Zeng-jie, LIU Bin, YU Zhan-hai.. Effects of Doxorubicin-loaded Mesoporous Silica Nanoparticles on Proliferation and Apoptosis of Human Tongue Cancer Tca-8113 Cells.[J]. Journal of Oral Science Research, 2017, 33(3): 249-253.

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