Promotion of IGF-1 on the Proliferation and Alkaline Phosphatase Activity of Dental Pulp Cells through PI3K/AKT Signaling Pathway in Vitro.

doi:10.13701/j.cnki.kqyxyj.2017.05.018

Abstract

Abstract: Objective: To study the effect of IGF-1 on the proliferation and alkaline phosphatase activity of dental pulp cells and its influences on PI3K/AKT signal pathway. Methods: Primary human dental pulp cells were isolated and cultured by enzymatic digestion. IGF-1 protein expression in dental pulp tissue was detected by Western blot. The dental pulp cells were then treated with IGF-1 at different concentrations, and cell proliferation was detected by MTT assay after 7 days. The dental pulp cells were treated with IGF-1 (100ng/mL) and LY294002 (10μmol/L) respectively or simultaneously for 7 days, and cell proliferation was detected by MTT assay after 7 days. ALP activity was detected after culture for 3, 5, 7 and 14 days. The protein expression of AKT and p-AKT was detected after culture for 7days. Results: IGF-1 showed Low expression in pulpitis. IGF-1 could significantly promote the proliferation of dental pulp cells in the concentration range of 20-100 ng/mL since 3rd day(P<0.05)with a dose- and time-dependent effect. LY294002 could inhibit the proliferation of dental pulp cells and alkaline phosphatase activity with time-dependence. IGF-1 could promote the expression of p-AKT protein, while LY294002 could reduce the effect of IGF-1 on the expression of p-AKT protein. Conclusion: IGF-1 can promote the proliferation and alkaline phosphatase activity of dental pulp cells in concentration and time dependence manner, which may be related to the PI3K/AKT signaling pathway.

CLC Number:

R781.3

DU Xiao-ying, DING Wei-wei, CHEN Yue, CHI Dan-dan.. Promotion of IGF-1 on the Proliferation and Alkaline Phosphatase Activity of Dental Pulp Cells through PI3K/AKT Signaling Pathway in Vitro.[J]. Journal of Oral Science Research, 2017, 33(5): 538-541.

References

[1] 王冉旭,潘爽,何丽娜,等.人牙髓干细胞在不同 HA含量的 PLGA/HA 复合支架材料上的粘附研究[J].口腔医学研究,2015,31(12)∶1171-1173
[2] Al-Kharobi H, El-Gendy R, Devine D A, et al. The role of the insulin-like growth factor (IGF) axis in osteogenic and odontogenic differentiation [J]. Cellular and molecular life sciences, 2014, 71(8)∶1469-1476
[3] 陈巍.胰岛素样生长因子-1和转化生长因子-β1在来曲唑诱导多囊卵巢综合征模型大鼠中的表达及意义[J].中国老年学杂志,2016,36(14)∶3395-3397
[4] 王雪.IGF-1通过JAK-STAT通路促进牙髓细胞增殖和分化的体外研究[D].河北医科大学,2015
[5] Chang M C, Hung H P, Lin L D, et al. Effect of interleukin-1β on ICAM-1 expression of dental pulp cells: role of PI3K/Akt, MEK/ERK, and cyclooxygenase [J]. Clinical oral investigations, 2015, 19(1)∶117-126
[6] Chen J, Duan Y, Zhang X, et al. Genistein induces apoptosis by the inactivation of the IGF-1R/p-Akt signaling pathway in MCF-7 human breast cancer cells [J]. Food & function, 2015, 6(3)∶995-1000
[7] Kadri Z, Lefevre C, Goupille O, et al. Erythropoietin and IGF-1 signaling synchronize cell proliferation and maturation during erythropoiesis [J]. Genes & development, 2015, 29(24)∶2603-2616
[8] 王雪鹏,李茂强,边振宇,等.PI3 K/Akt信号通路在骨髓间充质干细胞增殖及成骨分化调控中的作用[J].中华骨质疏松和骨矿盐疾病杂志,2014,7(3)∶250-257
[9] 关祥艳,李启梦,刘惠娟,等.PI3K/AKT信号通路对体外人牙髓细胞增殖和成牙本质向分化能力的影响[J].中华口腔医学研究杂志:电子版,2014,8(3)∶10-13
[10] Tapia O, Riquelme I, Leal P, et al. The PI3K/AKT/mTOR pathway is activated in gastric cancer with potential prognostic and predictive significance [J]. Virchows Archiv, 2014, 465(1)∶25-33
[11] Ren J G, Seth P, Clish C B, et al. Knockdown of Malic Enzyme 2 Suppresses Lung Tumor Growth, Induces Differentiation and Impacts PI3K/AKT Signaling [J]. Scientific Reports, 2014, 4(4)∶5414-5414
[12] 罗裕旋,吴传安,杨伟康,等.骨碱性磷酸酶 N-端骨钙素^ 99Tc^ m-亚甲基二膦酸盐骨显像与早期骨代谢改变[J].山西医药杂志, 2015, 44(24)∶2859-2861
[13] Aslantas E E, Buzoglu H D, Karapinar S P, et al. Age-related Changes in the Alkaline Phosphatase Activity of Healthy and Inflamed Human Dental Pulp [J]. Journal of endodontics, 2016, 42(1)∶131-134

[1]	LI Jing-jing, CHEN Su-lei, LI Jun-liang, QIAN Ya-jie, GAO Ya-fan, YANG Wei-dong. Evaluation of Apical Overfilling and Sealing with 4 Different Obturation Techniques in Oval-root Canals In Vitro [J]. Journal of Oral Science Research, 2017, 33(10): 1036-1039.
[2]	SHAO Li-na, WANG Si-mo, LI Xiao-lin, QIU Li-hong, YU Bo.. Expression and Clinical Significance of miR-146a-5p in Chronic Periapical Lesions. [J]. Journal of Oral Science Research, 2017, 33(6): 650-653.
[3]	HE Qiong-qiong, YANG Ying, HUANG Sheng-Fu, FAN Ming-wen.. Expression of TLR4, NOD2 and NLRP3 in Pulpitis. [J]. Journal of Oral Science Research, 2017, 33(5): 496-499.
[4]	XIAO Yan, SHEN Kai-qi.. Three-dimensional Finite Element Analysis of Different Root Canals Restored with 3D Printing Fiber Post. [J]. Journal of Oral Science Research, 2017, 33(3): 273-277.
[5]	YU Xiao-qing, LIU Yu-fei, GUO Shi-bo, FENG Rui-ming, ZHU Meng-ying, XUE Ming.. Comparison of Shaping Ability of Five Ni-Ti Rotary Instruments in Simulated Root Canals. [J]. Journal of Oral Science Research, 2017, 33(3): 286-289.
[6]	ZHANG Ming, FENG Yan. Clinical Evaluation of Pulp Revascularization for Immature Teeth with Wide Apical Foramen. [J]. Journal of Oral Science Research, 2017, 33(2): 175-178.
[7]	SHEN Su-qian, LI Juan, HUANG Shi-guang. Expression of Stem Cell Factor in the Periapical Tissues of Human Chronic Periapical Diseases. [J]. Journal of Oral Science Research, 2017, 33(2): 179-182.
[8]	AO Xiang, Wang Li-na, Wang Jiao-jiao, Ye Dan-dan, Ding Yin-hao, Qu Wen-shu, Niu Wei-dong. Association between Jak2 and Stat3 Expression in Experimental Induced Rat Periapical Lesions. [J]. Journal of Oral Science Research, 2017, 33(1): 1-5.
[9]	BAI Yu-hao, TANG Lu, MENG Xian-jie, ZHAO Ying. Anatomic Analysis of Root Canals of Chinese Mandibular Incisors by Micro-computed Tomography. [J]. Journal of Oral Science Research, 2017, 33(1): 70-73.
[10]	MEI Xue, XIE Li-ping, JIN You-hong, YE Fang, XIONG Wei, YUE Li, JIANG Shan.. Pulp Response of Beagle Dog to Direct Capping with iRoot BP Plus. [J]. Journal of Oral Science Research, 2016, 32(12): 1234-1239.
[11]	CHEN Yao, SHEN Jing.. Clinical Observation of GTR Combined with Bone Grafting in Endodontic Microsurgery. [J]. Journal of Oral Science Research, 2016, 32(12): 1269-1272.
[12]	CHEN Xia, YUAN Tong-sui, YE Fang, SUN Yu, YANG Jian, GUO Jun, JIN You-hong.. Expression of P2X Receptors in the Brainstem Induced by Dental Pulp Inflammation. [J]. Journal of Oral Science Research, 2016, 32(10): 1033-1037.
[13]	XUE Long, WANG Li-he, NIE Hong-bing, ZHOU Hai-jing, GUO Man-li, KANG Yong-jie, YANG Lan.. Antibacterial Effects of Lactobacillus Salivarius W22a on the Periodontal Pathogens. [J]. Journal of Oral Science Research, 2016, 32(10): 1042-1046.
[14]	YANG Xue-chao , ZHAO Shi-yong, JIANG Qian-zhou, YE Shang-zhu. A New Method for Minimally Invasive Apical Surgery with the Aid of Three-dimensional Printed Template [J]. Journal of Oral Science Research, 2016, 32(9): 944-948.
[15]	HU Shu-li,WANG Yan-qing,DONG Yan,ZHANG Hong-yan.. Expression and Clinical Significance of CD147 and CypA in Periapical Granulomas and Radicular Cysts. [J]. Journal of Oral Science Research, 2016, 32(7): 723-728.