Therapeutic Effect of Adenovirus-mediated HGF Transfection of ADSCs Combined with Injectable Hydrogel on the Repair of Temporomandibular Joint Arthritic Condylar Cartilage in Rabbits

doi:10.13701/j.cnki.kqyxyj.2017.09.004

Abstract

Abstract: Objective: To investigate the therapeutic effect of Ad-HGF-GFP of ADSCs combined with injectable hydrogel on the repair of damaged cartilage in the microenvironment of rabbit temporomandibular arthritis. Methods: ADSCs were isolated from rabbits and cultured in vitro. Cells were transfected with adenovirus-mediated HGF and injectable hydrogel were prepared. Then animals were randomly divided to five groups: group A (blank control); group B; group C (treated with ADSCs); group D (treated with ADSCs combined with injectable hydrogel); group E (treated with Ad-HGF-GFP-ADSCs combined with injectable hydrogel). All animals were sacrificed 3 or 9 weeks after surgery, and condylar cartilages were harvested and analyzed by scanning electron microscope, histomorphometric analysis, and real-time PCR. Statistical analysis was performed with SPSS 17.0 software and statistically significant differences were assessed by t-test. All statistical analysis was considered significant when P<0.05. Results: Electron microscope scanning and histomorphometric analysis indicated improvement of condylar cartilage in group D and E, compared to those in the group B and C. Real-time PCR showed that the

基金项目国家自然科学基金(编号:81170935)
作者简介张广德(1977~ ),男,山东人,学士,主治医师,主要从事口腔颌面外科临床治疗工作。
*通讯作者房殿吉,E-mail:dianjifang@yahoo.com.cn
expression of mmp13 gene in group E and A was comparable but decreased in group C and D, the difference was statistically significant. In contrast, the expression of TIMP-1 gene in group E and A was comparable but increased in group C and D, and the difference was statistically significant. Conclusion: Adenovirus-mediated HGF transfection of ADSCs combined with injectable hydrogel could promote the repair of temporomandibular joint arthritic condylar cartilage in rabbits.

Key words: ADSCs , Adenovirus, Hepatocyte growth factor(HGF), Injectable hydrogel, Temporomandibular joint , Osteoarthritis

CLC Number:

R782.6

ZHANG Guang-de, LI Rong-liang, YUE Cong-lei, YANG Shi-mao, GUO Yan-wei, FANG Dian-ji. Therapeutic Effect of Adenovirus-mediated HGF Transfection of ADSCs Combined with Injectable Hydrogel on the Repair of Temporomandibular Joint Arthritic Condylar Cartilage in Rabbits[J]. Journal of Oral Science Research, 2017, 33(9): 924-927.

References

[1] Lee HM, Joo BS, Lee CH, et al. Effect of glucagon-like peptide-1 on the differentiation of adipose-derived stem cells into osteoblasts and adipocytes [J]. Menopausal Med,2015,21(2)∶93-103
[2] Barretto LS, Lessio C, et al .Cell kinetics, DNA integrity, differentiation, and lipid fingerprinting analysis of rabbit adipose-derived stem cells [J]. In Vitro Cell Dev Biol Anim, 2014, 50(9)∶831-839
[3] 李龙,赵建辉,刘斌,等.兔脂肪干细胞的分离培养鉴定及诱导分化研究[J].口腔医学研究,2012,28(5)∶426-429
[4] Zanetti AS, McCandless GT, Chan JY, et al. In vitro human adipose-derived stromal/stem cells osteogenesis in akermanite:poly--caprolactone scaffolds [J]. Biomater Appl,2014 ,28(7)∶998-1007
[5] 陈慧,陈永进,张旻,等.脂肪干细胞应用于软骨缺损修复的研究新进展[J].口腔医学研究,2012,28(11)∶1203-1206
[6] 陈刚,胡波,王大章,等.颞下颌关节骨关节病动物模型的建立及组织病理学观察[J].实用口腔医学杂志,2003,17(6)∶5-7
[7] Barretto LS, Lessio C, et al. Cell kinetics, DNA integrity, differentiation, and lipid fingerprinting analysis of rabbit adipose-derived stem cells [J]. In Vitro Cell Dev Biol Anim, 2014, 50(9)∶831-839
[8] McGee SJ, Havens AM, Shiozawa Y, et al. Effects of erythropoietin on the bone microenvironment [J]. Growth Factors,2012,30(1)∶22-28
[9] Ren ML, Peng W, Yang ZL, et al. Allogeneic adipose-derived stem cells with low immunogenicity constructing tissue-engineered bone for repairing bone defects in pigs [J]. Cell Transplant,2012,21(12)∶2711-2721
[10] 杨丰建,俞永林,乔健,等.兔骨关节炎模型的建立以及关节炎软骨组织中 MMP1/13 的表达[J] .复旦学报:医学版,2007,34(4)∶563- 567
[11] Kim J, Kim HN, et al. Synergistic effects of nanotopography and co-culture with endothelial cells on osteogenesisof mesenchymal stem cells [J]. Biomaterials,2013,34(30)∶7257-7268
[12] Li C, Ding J, Jiang L, et al. Potential of mesenchymal stem cells by adenovirus-mediated erythropoietin gene therapy approaches for bone defect [J]. Cell Biochem Biophys,2014,70(2)∶1199-204

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