Role of Micro-34a in Induced-mineralization of Human Periodontal Ligament Cells

doi:10.13701/j.cnki.kqyxyj.2017.09.005

Abstract

Abstract: Objective: To investigate the effect of miR-34a on the osteogenic differentiation of human periodontal ligament cells (hPDLCs). Methods: HPDLCs were obtained through in vitro culture combined with enzyme digestion method and tissue pieces culture method. First, the cells were continuously induced by mineralization induction medium for 3,7, and 14 days. The expression of miR-34a was detected by quantitative real-time PCR (qRT-PCR). Second, hPDLCs were transfected with lentiviral vector containing pre-miR-34a or control to build the miR-34a overexpression model or control model (hPDLCs/34a or hPDLCs/pCDH) and induced by mineralization induction medium for 3, 7, 14, and 21 days. Quantification of osteogenensis-related genes (ALP, Runx2, OCN and BSP) expression, alkaline phosphatase (ALP) activity detection and Alizarin red staining were used to assess the influence of miR-34a on the osteogenic differentiation of hPDLCs. Results: The expression of miR-34a significantly increased in a time-dependent manner (P<0.001) during the osteogenic differentiation. The mineralization nodule formation and ALP activity were all attenuated by miR-34a. ALP gene expression detected by qRT-PCR was reduced after 7 days' induction in hPDLCs/34a. Runx2, OCN and BSP gene expression showed no significant

基金项目国家自然科学基金(编号:81200812)
武汉市应用基础研究计划项目(编号:2016060101010043)
作者简介程孟文(1995~ ),女,安徽阜阳人,硕士在读,主要从事口腔修复学研究工作。
*通讯作者周毅,E-mail: dryizhou@163.com
differences. Conclusion: MiR-34a may have a negative effect on the osteogenic differentiation of hPDLCs.

Key words: Human periodontal ligament cells , Osteogenic differentiation, MicroRNA-34a, Quantitative real-time PCR

CLC Number:

R329.2

CHENG Meng-wen, ZHOU Yi. Role of Micro-34a in Induced-mineralization of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2017, 33(9): 928-932.

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