Journal of Oral Science Research

Journal of Oral Science Research ›› 2018, Vol. 34 ›› Issue (12): 1333-1336.DOI: 10.13701/j.cnki.kqyxyj.2018.12.017

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Study on Mongersen's Anti-inflammatory Effect in Periodontal Inflammatory Microenvironment in Vitro.

WANG Liu-ran1, LIU Xin-chan2, MENG Yang1, DING Xiao-han1, WU Zhou3, YU Wei-xian4*   

  1. 1. Department of Periodontics, School of Stomatology, Jilin University, Changchun 130021, China;
    2. Department of Dental Implantology, School of Stomatology, Jilin University, Changchun 130021, China;
    3. Institute of Dentistry, University of Kyushu, Fukuoka-ken 812-8581, Japan;
    4. Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School of Stomatology, Jilin University, Changchun 130021, China.
  • Received:2018-06-10 Online:2018-12-28 Published:2018-12-27

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Abstract: Objective: To investigate the anti-inflammatory effect of mongersen, a Smad7 antisense oligonucleotide,in vitro.Methods: Lipofectamine TM 2000 contained mongersen was used to tansfect mouse macrophage (RAW264.7).Then RAW264.7 cells were co-cultured with Pg-LPS which was the main pathogen of periodontitis to simulate the periodontal inflammatory environment.After 12 hours, flow cytometry, western blot, and qRT-PCR were used to detect the transfection efficiency and expression of Smad7 protein and macrophages-related cytokines.Results: The transfection efficiency was 76.57% by flow cytometry.Mongeren could inhibit the protein expression of Smad7 by western blot.The expression of TNF-a, IL-6, IL-1β, and other pro-inflammatory cytokines were significantly reduced after adding mongersen.

Key words: Mongersen, Smad7, Macrophage, Periodontitis