Effect of Periodontal Intervention Supplemented with Antibiotics on Serum High Sensitivity C-reactive Protein and Carotid Artery in Rats with Periodontitis Associated with Type 2 Diabetes Mellitus

doi:10.13701/j.cnki.kqyxyj.2019.07.006

Abstract

Abstract: Objective: To investigate the effect of periodontitis and its intervention on vascular changes in rats with chronic periodontitis complicated with type 2 diabetes mellitus. Methods: Thirty-five male SD rats were randomly divided into three groups: group A (control group, 7 rats), group B (DM group, 7 rats), and group C (CP + DM group, 21 rats). Then group C was randomly divided into three groups: group C1 (natural process), group C2 (mechanical therapy plus antibiotics), group C3 (tooth extraction plus antibiotics), and 7 rats in each group. The levels of serum hsCRP were detected by enzyme-linked immunosorbent assay (ELISA). The pathological changes of the vessels were observed under light microscope after HE staining. Results: The levels of serum hsCRP in group C were significantly higher than those in group A and B at all time points (P<0.05). The levels of serum hsCRP in group C1 became higher gradually with time. One week after the first intervention, the levels of serum hsCRP in group C2 and C3 reached a peak and then began to decrease (group C2 suddenly recovered at the fifth time points). Histopathology showed that in group C1, the intima was incomplete and the smooth muscle tissue of the middle membrane was necrotic, showing amorphous granules and the vessel wall was thickened. In group C2 and C3, the intima was complete and the smooth muscle cells of the middle membrane were vacuolated. There was no obvious thickening of the vessel wall. In group B, the surface of intima was rough and the smooth muscle cells of the middle membrane were vacuolated. The vessel wall was thickened. In group A, there was no obvious abnormality about the vessel wall. Conclusions: For CP rats with DM, if no intervention is applied, the serum levels of inflammatory factors may be elevated due to the persistent development of periodontal inflammation and thus increasing the risk of diabetic angiopathy. Mechanical therapy and tooth extraction may increase the risk of carotid artery disease due to elevated levels of inflammatory factors in a short period of time. But in the long term effect, tooth extraction reduces levels of inflammatory factors and improves vascular disease by removing infected teeth

Key words: diabetes mellitus, periodontitis, high sensitivity C-reactive protein, periodontal intervention, carotid artery

ZHAO Yong, WANG Miaomiao, WANG Chong, LI Hao, GE Xuejun, REN Xiuyun. Effect of Periodontal Intervention Supplemented with Antibiotics on Serum High Sensitivity C-reactive Protein and Carotid Artery in Rats with Periodontitis Associated with Type 2 Diabetes Mellitus[J]. Journal of Oral Science Research, 2019, 35(7): 639-642.

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