Regulation of Odontoblastic Differentiation by miR-1895 in Mouse Dental Papilla Cells

doi:10.13701/j.cnki.kqyxyj.2021.01.007

Abstract

Abstract: Objective: To investigate the function of miR-1895 during the odontoblastic differentiation of mouse dental papilla cells. Methods: The model of odontoblastic differentiation of mouse dental papilla cells was established, and the expression of miR-1895 was detected during this process. The binding site of miR-1895 in Klf4 mRNA was analyzed by using bioinformatics. Western blot and real-time PCR were employed to detect the influence of miR-1895 on odontoblastic differentiation of mouse dental papilla cells after transfection with miRNA inhibitor. Results: The expression of miR-1895 decreased during the odontoblastic differentiation of mouse dental papilla cells. There was a binding site to miR-1895 in Klf4 mRNA. After inhibiting miR-1895, the expression of related genes of odontoblastic differentiation increased. Conclusion: miR-1895 inhibited the odontoblastic differentiation of mouse dental papilla cells and might participate in regulating this process.

Key words: dental papilla cells, odontoblastic differentiation, miR-1895

ZHANG Hao, YANG Guobin. Regulation of Odontoblastic Differentiation by miR-1895 in Mouse Dental Papilla Cells[J]. Journal of Oral Science Research, 2021, 37(1): 28-32.

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