Effects of Phillyrin on p38 MAPK/c-Fos Signal Pathway and Osteoclast Activation in Periodontitis Rats

doi:10.13701/j.cnki.kqyxyj.2021.01.008

Abstract

Abstract: Objective: To investigate the effects of phillyrin on p38 MAPK/c-Fos signal pathway and osteoclast activation in periodontitis rats. Methods: The periodontitis rat model were established and randomly divided into groups: model group, low dose phillyrin group, middle dose phillyrin group, high dose phillyrin group, and vitamin C group, with 12 in each group, and another 12 rats were set as control group. After treatment, methylene blue staining was used to detect alveolar bone resorption (distance from the border of enamel cementum to the crest of alveolar ridge). HE staining was used to detect the periodontal pathological morphology. The number of osteoclasts in periodontal tissue was detected by TRAP staining. The levels of serum tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were detected by enzyme-linked immunosorbent assay (ELISA). The expression of p38 MAPK/c-Fos pathway related proteins was detected by Western blot. Results: Compared with the control group, the periodontal tissue of the model group showed epithelial defect, rupture, abnormal morphology, disordered arrangement of periodontal membrane fibers, inflammatory cell infiltration, and other pathological damage, and the distance from the border of enamel cementum to the crest of alveolar ridge, the number of osteoclasts, the levels of TNF-α and IL-6, and the expressions of p38 MAPK/c-Fos pathway related proteins c-Fos and p-p38 MAPK/p38 MAPK in periodontal tissues were significantly higher (P<0.05). Compared with the model group, the pathological damage of periodontal tissue in the low, middle, and high dose phillyrin groups and vitamin C group was alleviated, the distance from the border of enamel cementum to the crest of alveolar ridge, the number of osteoclasts, the levels of TNF-α and IL-6, the expression of c-Fos protein and p-p38 MAPK/p38 MAPK in periodontal tissues were significantly lower (P<0.05), and phillyrin groups were dose-dependent (P<0.05), while there was no significant difference between high dose phillyrin group and vitamin C group (P>0.05). Conclusion: Phillyrin can down-regulate p38 MAPK/c-Fos signal pathway, inhibit periodontitis and osteoclast activation, reduce periodontal tissue damage, and improve periodontitis symptoms in rats.

Key words: phillyrin, periodontal tissue, inflammation, p38 MAPK/c-Fos signal, osteoclast activation

SU Juanjuan, ZHU Yongcui, ZHANG Wenling. Effects of Phillyrin on p38 MAPK/c-Fos Signal Pathway and Osteoclast Activation in Periodontitis Rats[J]. Journal of Oral Science Research, 2021, 37(1): 33-38.

References

[1] Li W, Zheng Q, Meng H, et al. Integration of genome-wide association study and expression quantitative trait loci data identifies AIM2 as a risk gene of periodontitis [J]. J Clin Periodontol, 2020, 47(5):583-593.
[2] Burcu K, David FL, Christopher JN, et al. Effects of smoking on non-surgical periodontal therapy in patients with periodontitis Stage Ⅲ or Ⅳ, and Grade C [J]. J Periodontol, 2020, 91(4):442-453.
[3] de Paiva Gonçalves V, Ortega AAC, Steffens JP, et al. Long-term testosterone depletion attenuates inflammatory bone resorption in the ligature-induced periodontal disease model [J]. J Periodontol, 2018, 89(4):466-475.
[4] Ishii T, Ruiz-Torruella M, Ikeda A, et al. OC-STAMP promotes osteoclast fusion for pathogenic bone resorption in periodontitis via up-regulation of permissive fusogen CD9 [J]. FASEB J, 2018, 32(7):4016-4030.
[5] Han SY, Kim YK. Berberine suppresses RANKL-induced osteoclast differentiation by inhibiting c-Fos and NFATc1 expression [J]. Am J Chin Med, 2019, 47(2):439-455.
[6] Tzeng HE, Tsai CH, Ho TY, et al. Radix paeoniae rubra stimulates osteoclast differentiation by activation of the NF-κB and mitogen-activated protein kinase pathways [J]. BMC Complement Altern Med, 2018, 18(1):132-140.
[7] 王慧,应俊,韩笑.三黄健齿汤结合西医常规疗法治疗慢性牙周炎临床研究[J].国际中医中药杂志,2018,40(5):410-413.
[8] 沈霞,徐蓉蓉,裴丽珊,等.基于网络药理学连翘清热解毒功效的分子机制研究[J].药学学报,2018,53(11):1834-1842.
[9] 祝媛,彭良哲.乙酰水杨酸预处理骨髓间充质干细胞对实验性牙周炎大鼠的治疗作用[J].基因组学与应用生物学,2020,39(1):308-313.
[10] 张震,张庆峰,李伟,等.连翘苷对创伤性骨折大鼠模型骨愈合作用的研究[J].免疫学杂志,2019,35(1):71-76.
[11] 黄捷,李庆华,任晓斌,等.辅酶Q10对大鼠实验性牙周炎的影响作用[J].中国民族民间医药,2018,27(5):25-32.
[12] 王柳然,刘歆婵,孟阳,等.Mongersen在牙周炎症微环境中抗炎作用的体外研究[J].口腔医学研究,2018,34(12):1333-1336.
[13] Chitsazi M, Faramarzie M, Sadighi M, et al. Effects of adjective use of melatonin and vitamin C in the treatment of chronic periodontitis: A randomized clinical trial [J]. J Dent Res Dent Clin Dent Prospects, 2017, 11(4):236-240.
[14] 彭曼斯,韩淋畴,何升腾,等.清胃固齿汤联合米诺环素治疗慢性牙周炎疗效以及对菌群代谢的影响[J].中华中医药学刊,2018,36(7):1739-1742.
[15] Kim K, Kim JH, Kim I, et al. Rev-erbα negatively regulates osteoclast and osteoblast differentiation through p38 MAPK signaling pathway [J]. Mol Cells, 2020, 43(1):34-47.