Role of SDC4 in Perineural Invasion of Salivary Adenoid Cystic Carcinoma

doi:10.13701/j.cnki.kqyxyj.2023.12.011

Abstract

Abstract: Objective: To investigate the role of SDC4 in the perineural invasion of salivary adenoid cystic carcinoma induced by CXCL12. Methods: Transwell assay was used to evaluate the influence of different concentrations of CXCL12 on the chemotaxis of SACC-83 cells. Immunofluorescence staining and co-immunoprecipitation were performed to validate the interactions between SDC4 and CXCL12. The expression of SDC4 in the SACC-83 cells was silenced by viral transfection by constructing lentivirus-expressing shRNA targeting SDC4. The effect of SDC4 silencing on the proliferation and migration was assessed via the CCK-8, wound healing, and transwell assays. An in vivo perineural invasion model was used to prove the effect of SDC4 silencing on the perineural invasion of the SACC-83 cells. Results: CXCL12 induced the migration of SACC-83 cell in a concentration-dependent manner (P<0.01). Co-immunoprecipitation studies validated these interactions between SDC4 and CXCL12. Furthermore, SDC4 gene expression was silenced by RNAi and verified by real-time polymerase chain reaction and western blotting analysis. The migration and chemotactic efficiency of the SACC-83 cells induced by CXCL12 were significantly decreased via SDC4 silencing (P<0.05). While the proliferation of the SACC-83-SDC4-silenced cells showed no difference compared to that of SACC-83 cells (P>0.05). The perineural invasion ability was significantly inhibited by SDC4 silencing in vivo (P<0.05). Conclusion: SDC4 involved in the perineural invasion of salivary adenoid cystic carcinoma through interaction with CXCL12.

Key words: salivary adenoid cystic carcinoma, SDC4, CXCL12, perineural invasion

ZHANG Yanning, SHI Hong, LI Hexiang, WANG Jie, HAO Yali, WANG Xu, YUAN Yunyun. Role of SDC4 in Perineural Invasion of Salivary Adenoid Cystic Carcinoma[J]. Journal of Oral Science Research, 2023, 39(12): 1085-1091.

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