MUC1 Promotes Growth and Invasion of Salivary Adenoid Cystic Carcinoma Cells via EGFR/ERK Signaling Pathway

doi:10.13701/j.cnki.kqyxyj.2022.01.006

Abstract

Abstract: Objective: To investigate the effects of MUC1 on the biological properties of salivary gland adenoid cystic carcinoma (ACC) cells and the underlying mechanisms. Methods: MUC1 silenced ACC cell line was constructed by lentivirus. Real-time PCR and western blotting were performed to evaluate the efficiency of MUC1 knock-down. CCK-8 assay was used to detect cell proliferation and plate colony assay was carried out to detect cell clone formation ability. Transwell assays were utilized to investigate cell migration and invasion ability. RNA-seq analysis was used to explore the molecular mechanisms regulated by MUC1. Results: MUC1 was knocked down in SACC cell by shRNA. The down-regulation of MUC1 attenuated the proliferation, migration, and invasion ability of SACC cells. RNA-seq analysis and western blot assay showed that MUC1 could regulate biological effects of SACC cells mainly via EGFR/ERK signaling pathway. Conclusion: MUC1 plays a crucial role in regulating ACC cell biological behaviors by regulating phosphorylation levels of EGFR/ERK and may play a role in the development of ACC.

Key words: Mucin1, salivary adenoid cystic carcinoma, proliferation, invasion

LU Hao, XU Wanlin, WU Yifan, ZHU Yun, LIU Shengwen, Yang Wenjun. MUC1 Promotes Growth and Invasion of Salivary Adenoid Cystic Carcinoma Cells via EGFR/ERK Signaling Pathway[J]. Journal of Oral Science Research, 2022, 38(1): 24-29.

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