Aberrant Expression of IGF2BP2 Promotes Proliferation and Invasion of Oral Squamous Cell Carcinoma

doi:10.13701/j.cnki.kqyxyj.2021.04.017

Abstract

Abstract: Objective: To explore the effects of aberrant expressed IGF2BP2 on proliferation and invasion of oral squamous cell carcinoma (OSCC). Methods: High-throughput RNA sequencing was performed on four pairs of OSCC tissues and adjacent normal tissues to screen differentially expressed RNA binding proteins. siRNA was used to reduce the expression of IGF2BP2 in OSCC cell lines. The effects of IGF2BP2 on proliferation and invasion were determined by CCK-8 and Transwell Assay. The target genes of IGF2BP2 were analyzed by Improved RIP-seq and mRNA-seq, and the main signal pathways of IGF2BP2 target genes were obtained by KEGG analysis. Results: The abundance and up-regulation of IGF2BP2 was the most significantly in the RNA-binding proteins in OSCC (P<0.01). The decreased IGF2BP2 inhibited the proliferation and invasion of OSCC cell lines. The target genes of IGF2BP2 were mainly enriched in Axon Guidance, Focal expression, Rap1 signaling pathway, Pathways in Cancer, and MAPK signaling pathway. Conclusion: IGF2BP2 is increased in OSCC and may promote tumor progression by affecting cell proliferation and adherence-related signaling pathways.

Key words: IGF2BP2, OSCC, proliferation, invasion

HU Yaying, CHEN Wen, ZHANG Jiali. Aberrant Expression of IGF2BP2 Promotes Proliferation and Invasion of Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2021, 37(4): 354-359.

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