Influence of Hyaluronic Acid on HMGB-1 Expression of Chondrocytes with IL-1β Stimulation.

doi:10.13701/j.cnki.kqyxyj.2018.03.007

Abstract

Abstract: Objective: To study the effect of hyaluronic acid (HA) on HMGB-1 expression of chondrocytes with IL-1β stimulation. Methods: TMJ condylar chondrocytes were obtained through in vitro culture. HA and glycyrrhizin (GL) were respectively incubated in chondrocytes with IL-1β stimulation. Total RNA and protein were extracted 24h after stimulation to detect the mRNA expression of HMGB-1 and receptors, and protein expression of HMGB-1. Results: After IL-1β stimulation, the mRNA expressions of HMGB-1, TLR2, and TLR4 were increased. The mRNA expressions of HMGB-1, TLR2, and TLR4 were significantly decreased with the addition of HA or GL in contrast to only IL-1β stimulation (P<0.05). Also the protein expression of HMGB-1 was decreased after HA or GL stimulation (P<0.05). Conclusion: HA could decrease the HMGB-1 and receptors expression of chondrocytes in IL-1β stimulation.

Key words: Condyle, Chondrocyte, Hyaluronic acid, HMGB-1, IL-1β

LI Cheng, DENG Mo-hong, GUO Hui-lin, FENG Ya-ping, FANG Wei, LONG Xing. Influence of Hyaluronic Acid on HMGB-1 Expression of Chondrocytes with IL-1β Stimulation.[J]. Journal of Oral Science Research, 2018, 34(3): 241-244.

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