Extra Domain A Positive Fibronectin Promotes Osteoclast Formation and Bone Destruction of Radicular Cysts.

doi:10.13701/j.cnki.kqyxyj.2018.09.008

Abstract

Abstract: Objective: To detect the expression of the isoforms of fibronectin (FN) variable splicing subtype protein in radicular cysts, and to study the ability of each subtype in inducing osteoclasts in vitro, and to analyze the association of these isoforms with osteoclastogenesis. Methods: Specimens from 8 patients with radicular cysts were selected. Immunohistochemistry was used to study the expression of each isoforms in the fibrous capsule. Fibroblasts were isolated from surgical samples, the mRNA levels of each isoform and total FN were detected by RT-PCR, and the conditioned medium was collected to induce the osteoclasts in vitro. The association between FN isoforms and osteoclastogenesis was analyzed. Results: In the fibrous capsule of radicular cysts, FN containing the extra domain A (EDA+FN) was stained more intense than EDB+FN and CS1-FN. Consistently, the mRNA level of EDA+FN was also significantly higher than that of EDB+FN (P=0.007) and CS1-FN (P=0.003) in the fibroblasts. The ratio of EDA+FN/total FN was also the highest among three isoforms (EDB+FN/total FN,P<0.001 and CS1-FN/total FN, P<0.001). Only EDA+FN/total FN was positively associated with the Trap+MNC numbers induced by the conditioned medium (n=8, R=0.776, P=0.024). Conclusion: The fibroblasts in the fibrous capsule of radicular cysts generate various FN isoforms, in which EDA+FN consisted of the majority and positively associated the oste-
oclastogenesis induced by fibroblasts. It is suggested that the fibroblasts within the stroma made the microenvironment favourable to the bone destruction of radicular cysts.

Key words: Fibronectin Extra domain, A Alternative splicing , Osteoclasts , Radicular cyst

CHEN Ya-wen,WANG Hai-Cheng, DONG Wei-jie.. Extra Domain A Positive Fibronectin Promotes Osteoclast Formation and Bone Destruction of Radicular Cysts.[J]. Journal of Oral Science Research, 2018, 34(9): 952-955.

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