Mineralization of A Novel Immortalized Cementocyte Cell Line: A Pilot Study.

doi:10.13701/j.cnki.kqyxyj.2018.12.015

Abstract

Abstract: Objective: To investigate the mineralization of a novel murine immortalized cementocyte-like cell line IDG-CM6.Methods: In vivo, three-month-old C57BL/6 male mice were sacrificed to collect the cellular cementum and alveolar bone around the mesial root of the first mandibular molar for scanning electron microscopy (SEM) and Raman spectroscopy.In vitro, IDG-CM6 cells were cultured for 3, 7, 14, 21, and 28 days to compare with osteoblast/osteocyte cell line IDG-SW3.Alizarin red staining, SEM, and Raman spectroscopy were used to analyze the cells.Results: Both two cells mineralized during the cell culture.However, the alizarin red staining showed significant calcium deposition in IDG-CM6 cells compared with IDG-SW3 cells at the same time course.SEM showed that there was no obvious difference in Ca/P ratio between two kinds of cells, consistent with that of the cellular cementum and alveolar bone.Raman analysis indicated that the PO₄^3-/CH₂ of IDG-CM6 cells was significantly lower than that of IDG-SW3 cells, consistent with the markedly lower PO₄^3-/CH₂ (1.76) of cellular cementum than that of alveolar bone (4.76).Conclusion: IDG-CM6 cells might be an ideal model to study the mineralization and biological characteristics of cementum.

Key words: Cementocyte, Mineralization, SEM, Raman spectroscopy

WANG Xin, ZHANG Cheng, LI Tian-cheng, TAO Gui-yu, DUAN Pei-pei, ZOU Shu-juan. Mineralization of A Novel Immortalized Cementocyte Cell Line: A Pilot Study.[J]. Journal of Oral Science Research, 2018, 34(12): 1324-1328.

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