Effect of Semiconductor Laser Irradiation on Oral Ulcer Model of SD Mice

doi:10.13701/j.cnki.kqyxyj.2020.08.016

Abstract

Abstract: Objective: To investigate the effect of semiconductor laser with different parameters on levels of β-endorpin (β-EP), 5-hydroxytryptamine (5-HT), and healing of oral ulcers in SD rats. Methods: Nine rats were randomly selected from 60 SD rats and set as the normal control group. The remaining 51 rats were constructed oral ulcer models and the modeling day was set as day 0. 3 rats were selected for pathological observation to determine the success of model construction. The remaining 48 ones were randomly divided into four groups: ulcer control group and three laser irradiation groups. The three laser groups were given laser therapy at 1.0W/20Hz, 1.5W/20Hz, and 2.0W/20Hz (once per day, 3 consecutive days). The levels of β-EP and 5-HT were detected at 24 h after the last treatment. The healing of the ulcers was observed and evaluated by healing index following by microscope observation on day 2, 5, and 9. Results: The level of β-EP was increased in ulcer tissues (P<0.01). There was no statistically significant difference in the β-EP level between the laser groups and the ulcer control group. No statistically significant difference was found in the content of 5-HT between all groups (P>0.05). The healing index was better in laser groups on day 5, and the hyperemia in laser groups was significantly reduced. Besides, the pseudomembrane was thinner, and there was a large number of neovascularization and collagenous fibers observed by microscope. On day 9, most ulcers were healed in the laser groups. However, congestion and edema was more obvious in the 2W group than the other two groups. Healing index was better in 1W and 1.5W groups than other groups, however, more keratinization showed in 1.5W. Epithelium covered about 90% of the ulcer surface in 2W group. Conclusion: Laser therapy can not increase the release of β-EP in ulcer tissues, and has no effect on 5-HT content. Laser irradiation can significantly promote the healing of oral ulcer, and the healing is better in the 1.5W group, suggesting that 1.5W/20Hz may be a proper parameter for clinical application of semiconductor laser in the treatment of oral ulcer.

Key words: semiconductor laser, oral ulcer, pain, healing

HUO Xiao, HAN Ning, LIU Li. Effect of Semiconductor Laser Irradiation on Oral Ulcer Model of SD Mice[J]. Journal of Oral Science Research, 2020, 36(8): 770-775.

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