Effects of YAP Inhibition on Proliferation and Apoptosis of Fibrocartilage Stem Cells

doi:10.13701/j.cnki.kqyxyj.2021.11.012

Abstract

Abstract: Objective: To investigate the effects of YAP inhibition on the proliferation and apoptosis of fibrocartilage stem cells (FCSCs), and bring new insights for pathology of TMJOA. Methods: FCSCs were isolated from SD rats and identified. Synthesized sequences of siRNA were transfected into FCSCs in vitro for YAP inhibition. The proliferation activity of FCSCs after transfection was detected by using cell counting kit-8 (CCK-8). Changes in the cell cycle and apoptosis rate of FCSCs were detected by using flow cytometry. TMJOA model was conducted in rats, and verteporfin (a YAP inhibitor) was intra-articular injected, whose effect on the number of FCSC was detected by immunofluorescent costaining and compared with TMJOA control group. Results: After transfection, FCSC's proliferation activity was significantly inhibited (P<0.05) and apoptosis rate was increased (P<0.05). The cell cycle was changed as the proportion of G0/G1 increased (P<0.05) and S phase decreased (P<0.05). Immunofluorescent staining demonstrated that FCSC were decreased during TMJOA, which was intensified in the YAP inhibition group and resulted in a more depleted FCSC pool. Conclusion: The inhibition of YAP expression can decrease the proliferation activity, induce the apoptosis, and change the cell cycle of FCSCs. The consequent intensified FCSC pool depletion may be a critical reason for the progression of TMJOA.

Key words: fibrocartilage stem cell, temporomandibular joint, YAP, stem cell regulation

QIN Haoyang, HUANG Shun, JI Ping, ZHANG Shuqi, REN Xiaochun. Effects of YAP Inhibition on Proliferation and Apoptosis of Fibrocartilage Stem Cells[J]. Journal of Oral Science Research, 2021, 37(11): 1017-1022.

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