Abstract: Objective: To establish tongue cancer cell line and investigate the development of tongue cancer bioluminescence imaging in nude mice. Methods: Liposome plasmid carring the luciferase gene was transfected into Tca83 cells and G418 was used to select these cells. The cultured cells were transplanted into nude mice. Cancer cells was monitored and characterized by HE staining. Results: The monoclonal cell line expressing luciferase stably was obtained and monitored in vivo by bioluminescent imaging technology. Conclusion: This study provides a new way to investigate the mechanism of cancer development and metastasis.