Journal of Oral Science Research

Effects of MT01 on the Expression of Specific Osteoblast-related Factors in MG63 Infected by Porphyromonas Gingivalis.

ZHOU Yue, SHEN Yu-qin, GAO Han, LIU Yin, YU Hai-jiao, LIN Chong-tao.

2016, 32(1): 1-4. DOI: 10.11839/kqyxzh.2016.01.001

Abstract ( 228 )

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Objective: To explore the effects of MT01 on the differentiation of osteoblasts by determining the expression of specific osteoblast-related factors in MG63 infected by porphyromonas gingivalis. Methods: Human osteoblast-like cells MG63 were seeded into 6 well plates. Specific sequence oligodeoxynucleotide MT01 at a concentration of 1 μg·mL^-1 and the same volume of PBS were added separately, and the cells were incubated for 3 hours. Then bacterium suspension of P.g at multiplicity of infection MOI=100∶1 was added. The same volume of PBS was added in the control group. Four groups were set, i.e. MT01+P.g, MT01, P.g, and blank control group. Real-time quantitative polymerase chain reaction was used to detect the mRNA expression of osteoblast-related factors Runx2, SP7 at 2, 4, 6, 8, 12, and 24 hours respectively. Results: The mRNA levels of osteoblast-related factors in MG63 were markedly upregulated after adding MT01 following infected by P.g or not. However, the ability of MT01 to upregulate the expression of Runx2 and SP7 at different time-points was different. Conclusion: MT01 could promote the differentiation of osteoblasts in infected status.

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E3 Ubiquitin Ligase RNF126 Regulates the Invasion of Tongue Cancer Cells.

LIU Yu, YIN Wei, ZHAO Yue-han, SHI Chun.

2016, 32(1): 5-7. DOI: 10.11839/kqyxzh.2016.01.002

Abstract ( 347 )

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Objective: To investigate the effects of RNF126 on tumorigenesis of tongue cancer. Methods: The cell proliferation of SCC25 was determined by MTT. Single cell variation of SCC25 was determined by colony formation assay. The migration ability was analyzed by Transwell assay. Results: Overexpression of RNF126 promoted the proliferation of SCC25 cell, while inhibition of RNF126 decreased the SCC25 cell growth. Inhibition of RNF126 also decreased the migration ability of SCC25 cell. Conclusion: These data suggested that RNF126 might be related to the invasion of tongue cancer.

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Alternative Splicing of SPAG5 Exon3 is Regulated by SRSF3 in Oral Squamous Cell Carcinoma.

LIU Pei-qi, SHI Li-ming, JIA Rong.

2016, 32(1): 8-11. DOI: 10.11839/kqyxzh.2016.01.00

Abstract ( 384 )

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Objective: To study the effect of SRSF3 on alternative splicing of SPAG5 exon3 in oral squamous cell carcinoma (OSCC). Methods: SRSF3 was knocked down using siRNA in OSCC CAL27 cells. Alternative splicing of SPAG5 exon3 was analyzed by RT-PCR. Results: Knockdown of SRSF3 inhibited the expression of full length SPAG5 and increased short spliced product of SPAG5 in CAL27 cells. Conclusion: SRSF3 could down-regulate the expression of SPAG5 protein and interfere with mitosis in OSCC.

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Effect of Fiber Laser Etching on the Properties of Zirconia Ceramics.

LIN Ting-ting,FAN Jiang-yuan,ZHENG Rui-bin,WANG Si-qian,HU fang-xuan,MA Jian-feng.

2016, 32(1): 12-16. DOI: 10.11839/kqyxzh.2016.01.004

Abstract ( 223 )

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Objective: To evaluate the effect of fiber laser systems on flexural strength and shear bond strength of zirconia ceramics. Methods: Fifty sintered zirconia ceramic blocks were prepared and divided into five groups (n=10). Group A: control group without treatment, Group B: air abrasion with Al2O3 particles, Group C to E: fiber laser with 2W, 4W, 6W power respectively. The flexural strength of all samples was measured and the microscopic morphology of each group was observed under SEM. After that, dual-cure composite resin cement Multilink N was bonded to the ceramic specimens and stored in distilled water at 37℃ for 24h. Mean values of shear bond strength (MPa) were calculated. Data were analyzed by one-way ANOVA and LSD tests (P<0.05). Results: The flexural strength did not show significant difference among the groups. The bond strength of group A and E were lower than that of group B, C, D (P<0.05). No significant differences were found among group B, C, D. Conclusion: This study shows that with proper laser power, fiber laser treatment can increase the bond strength of zirconia compared to the untreated group without affecting the flexural strength of the ceramics. Fiber laser may be an alternative method to pretreat zirconia ceramics in the clinic.

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Preparation and Anti-inflammatory Effects of Nanoparticles Loaded with PMX-53.

LI Na, NI Shi-lei, HOU Yu-bo, LIU Xin-chan, TANG Qiu-ling, Pan Jia-hui, LI Ge-ge, YU Wei-xian.

2016, 32(1): 17-20. DOI: 10.11839/kqyxzh.2016.01.005

Abstract ( 229 )

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Objective: To prepare a sustained-release nanoparticles loaded with PMX-53. Methods: Nanoparticles loaded with PMX-53 were prepared by a two-step desolvation process. The morphology of the nanoparticles was observed by SEM. The encapsulation rate, drug content and the drug release were tested by HPLC. RAW264.7 cells were cocultured with gingipains to simulate inflammation environment and study the anti-inflammatory effects of drug-loaded microparticles in vitro. The experiment was divided into 4 groups: control group, gingipains group, gingipains+PMX-53 (1mg/L) group, and gingipains+nanoparticles loaded with PMX-53 (4.17g/L) group. The levels of IL-1β and IL-6 were detected after 48 hours by enzyme linked immunosorbent assay (ELISA). Results: SEM revealed that nanoparticles loaded with PMX-53 were spherical and 51.24% of them were 40~60nm in diameter. Drug content was 0.24mg/g. The encapsulation rate was 46.2%. The release cycle was up to seven days. The levels of IL-1β and IL-6 of gingipains group were higher than those of control group after 48 hours by ELISA. The levels of IL-1β and IL-6 of gingipains+nanoparticles loaded with PMX-53 group and gingipains+PMX-53 group were lower than gingipains group. Furthermore, there was a significant reduction in the secretion of IL-6 of gingipains+nanoparticles loaded with PMX-53 group. Conclusion: Nanoparticles loaded with PMX-53 showed good anti-inflammatory effects.

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Formation of Alendronate Sodium and Collagen Type I Complex Films on the Nano-hydroxyapatite Surface.

JIANG Yan, YAO Jiang-Wu.

2016, 32(1): 21-25. DOI: 10.11839/kqyxzh.2016.01.006

Abstract ( 246 )

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Objective: To form a stable and repeatable alendronate sodium (ALN) and collagen type I (COL I) complex films on nano-hydroxyapatite (nHA) layer. Methods: A nHA layer was coated on the pure titanium (PT) surface by using electrochemical deposition, then ALN and COL I were chemically bonded on the nHA layer to form nHA/ALN/COL I and nHA/COL I/ALN complex films using 3-aminopropyl triethoxysilane (APTES) and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) as coupling agent. The nHA layer was characterized by scanning electron microscope (SEM), energy dispersive X-ray spectroscopy (EDS) and X-ray diffractometer (XRD), and the complex films were characterized by X-ray photoelectron spectrometer (XPS) and Fourier transform infrared spectroscopy (FTIR). Results: ALN and COL I were bonded on the nHA layer by coupling agent, nHA/ALN/COL I and nHA/COL I/ALN complex films were formed by changing the binding order of ALN and COL I. Conclusion: The nHA/ALN/COL I and nHA/COL I/ALN complex films formed by chemical covalent bonding method have different potentials of ALN sustained-release.

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Biocompatibility of Ultrafine-grained Titanium Used for Implant after Sandblasting and Large-grits Acid-etching.

AN Bai-li, LI Zhi-rui, DIAO Xiao-ou, XIN Hai-tao, GUO Ya-zhou, WU Yu-lu.

2016, 32(1): 26-30. DOI: 10.11839/kqyxzh.2016.01.007

Abstract ( 330 )

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Objective: To study the effect of sandblasting and large-grits acid-etching (SLA) on the adhesion and proliferation of MC3T3-E1 cells on ultrafine-grained titanium (UFG Ti). Methods: Ti cylindrical specimen with 6mm in diameter and 3mm in height were incised from the bars of UFG Ti and Pure Ti. Experimental group (SLA-UFG Ti) was UFG Ti through SLA, while control group were UFG Ti through mechanical processing without any advanced processing (M-UFG Ti) and Pure Ti through SLA (SLA-Ti). The surface morphology and hydrophily of the surfaces were analyzed. The MC3T3-E1 cells were seeded on the surfaces of titanium specimens. Cell initial adhesion, cell density, and cell viability were observed. Results: Plenty of tiny sandpits with irregular shape were formed on the surface of SLA-UFG Ti group. Hydrophily of UFG Ti was improved after Equal-Channel Angular Pressing processing (ECAP). At the early stage, cell adhesion on the surface of SLA-UFG Ti was better than that of control group, while cell density was more intensive in the middle stage and the later stage. Cell viability was better at the middle stage, while no significant difference at the later stage. Conclusion: The surface property of SLA-UFG Ti was improved. SLA-UFG Ti could induce adhesion and proliferation of MC3T3-E1 cells. It might be a prospective substitute to pure Ti implant.

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Expression of E-cadherin mRNA in the Rat Tongue Carcinogenesis.

TAN Hong, MAO Liang, WANG Min, LIAO Peng-cheng, NIE Min-hai.

2016, 32(1): 31-34. DOI: 10.11839/kqyxzh.2016.01.008

Abstract ( 254 )

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Objective: To investigate the expression and significance of E-cadherin mRNA in the rat tongue carcinogenesis. Methods: 4NQO Drinking Water Act was used to induce tongue squamous cell carcinoma of rat. The mRNA expression level of E-cadherin was analyzed by q-RT-PCR. Results: The mRNA expression of E-cadherin was down-regulated in the rat tongue carcinogenesis. In the epithelial hyperplasia tissues, mild epithelial dysplasia tissues, moderate to severe epithelial dysplasia tissues and squamous cell carcinoma tissues, E-cadherin mRNA level was 0.453541, 0.207062, 0.190954, 0.180987 times by the normal epithelial tissues, respectively. The differences between squamous cell carcinoma group and normal epithelial group were statistically significant (P＜0.05). Conclusion: With the increase of pathological grade, E-cadherin mRNA expression showed a decreasing trend. The change of E-cadherin mRNA expression was an early event in rat tongue mucosa carcinogenesis.

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Comparison of Prognosis of Beagle Dogs' Endodontic Microsurgery between Cone Beam CT and Periapical Radiography.

GAO Jing, SHEN Jing, ZHANG Hai-feng, JIN Shu-feng.

2016, 32(1): 35-38. DOI: 10.11839/kqyxzh.2016.01.009

Abstract ( 261 )

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Objective: To compare the prognosis assessment of endodontic microsurgery treatment for beagle dogs' experimental periodontitis between cone beam CT (CBCT) and periapical radiographs (PRs). Methods: Pulps of 18 premolars in 3 beagle dogs were exposed to oral environment for 8 weeks. All 36 roots of the premolars developed apical periodontitis confirmed by both PRs and CBCT. All teeth received root canal treatment, followed by endodontic microsurgery. PRs and CBCT were performed both immediately and 6 months after endodontic microsurgery. The ability to detect periapical bone defect was compared accroding to apical radiolucent lesion area on the radiographic films of two methods. Results: Immediately after endodontic microsurgery, the areas of apical radiolucent lesions in both CBCT sagittal and coronal plane were greater than those in PRs (P=0.000, P=0.026). There was no significant difference between CBCT sagittal and coronal plane (P=0.070). 6 months after endodontic microsurgery, the area of apical radiolucent lesions in both CBCT sagittal and coronal plane were greater than those in PRs (P=0.000, P=0.012). The area of apical radiolucent lesions in CBCT sagittal plane was significantly greater than that in coronal plane (P=0.001). Conclusion: CBCT is better than PRs in detecting periapical bone defect, and can serve as an effective evaluation method in the evaluation of endodontic microsurgery.

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Characteristic and Application of Transplanted Tumor Imaging of Tongue Cancer.

JIN Zhi-meng, WANG Tong, HAO Wei-liang, YANG Li, SHEN Jun.

2016, 32(1): 39-42. DOI: 10.11839/kqyxzh.2016.01.010

Abstract ( 222 )

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Objective: To establish tongue cancer cell line and investigate the development of tongue cancer bioluminescence imaging in nude mice. Methods: Liposome plasmid carring the luciferase gene was transfected into Tca83 cells and G418 was used to select these cells. The cultured cells were transplanted into nude mice. Cancer cells was monitored and characterized by HE staining. Results: The monoclonal cell line expressing luciferase stably was obtained and monitored in vivo by bioluminescent imaging technology. Conclusion: This study provides a new way to investigate the mechanism of cancer development and metastasis.

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Clinical Effect Evaluation of IPS E. max Press Ceramic Endocrown Restoring Posterior Teeth after Root Canal Therapy.

ZENG Chang, FAN Ming-wen, ZHU Ling-xin, XU Qing-an.

2016, 32(1): 43-45. DOI: 10.11839/kqyxzh.2016.01.011

Abstract ( 301 )

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Objective: To evaluate the clinical effect of IPS e. max press ceramic endocrown restoring posterior teeth after root canal therapy. Methods: Twenty-six posterior teeth after root canal therapy were restored by endodontic first-year graduate students with IPS e. max Press ceramic endocrown. The patients were recalled 18-24 months after the restoration and the restorations were assessed using the modified USPHS criteria including secondary caries, gingivitis, tooth fracture, marginal discoloration, marginal integrity criteria, and restoration fracture/debond. Results: Of all the 26 teeth, one restoration was found broken. Two restorations had slight damage of marginal integrity and one had slight damage of occlusal surface. Other defects of endocrowns, or tooth and gingiva problems caused by the restorations were not found. Conclusion: Endocrown is an effective method of restoring posterior tooth after root canal therapy.

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Osteotome Sinus Floor Elevation and Simultaneous Dental Implantation without Bone Grafts - A 3-Year Retrospective Study

ZHANG Xing, LIU Ying, CHEN Song-ling, CHEN Jian-ling.

2016, 32(1): 46-49. DOI: 10.11839/kqyxzh.2016.01.012

Abstract ( 326 )

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Objective: To evaluate the clinical and radiographic outcomes of the osteotome sinus floor elevation technique and simultaneous dental implantation without grafting. Methods: The study population comprised 38 patients in whom 58 implants were inserted with the osteotome sinus floor elevation technique without grafting. Implant survival rate and radiological examination of all implants were estimated after 6 months, 1 year and 3 years. Result: One implant was off at the 1-year follow-up, and one more at the 3-year examination. The remaining 56 implants inserted were in function, giving a 3-year cumulative survival rate of 96.6%. The mean height of the alveolar process at the intended implant sites was 5.8±0.2 mm, and the mean elevation of the sinus floor was 5.1±0.3 mm. New bone regeneration around implant was found in sinus by radiological examination. The mean high of the new bone was 2.4mm at the 6-month follow-up, and 2.8mm at the 1-year. There was no significant difference between 1-year and 3-year. Conclusion: The osteotome sinus floor elevation technique with simultaneous dental implantation without grafting is feasible. The present study supports the theory that maxillary sinus mucosa has a great potential of bone formation.

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Esthetic Outcome of Single Implant Crowns following Immediate Implant Treatment and Delayed Implant Treatment in the Anterior Maxillary.

XIA Ting, SHI Bin.

2016, 32(1): 50-54. DOI: 10.11839/kqyxzh.2016.01.013

Abstract ( 303 )

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Objective: To compare the esthectic outcome of single implant crowns following immediate implant treatment (IIT) and delayed implant treatment (DIT) in the anterior maxillary. Methods: Forty-two dental implants were included in the study, 20 implants received immediate implant treatment and 22 implants received delayed implant treatment. Intra-oral digital photographs were obtained 6 months after final restoration. Pink esthetic score (PES) and white esthetic score (WES) were calculated. Statistical analysis was performed to compare the parameters between two groups. Results: The implant survival of IIT was 95.0% and that of DIT was 100%. There was no significant difference between two groups. Mean values of PES were 12.05±0.97 for IIT and 10.50±1.57 for DIT. Mean values of WES were 8.00±1.41 for IIT and 6.95±1.21 for DIT. Significant differences were found between the two groups in PES and WES. 68.4% of the IIT group showed perfect pink esthetic outcome (PES≥12), but only 22.7% in the DIT group. These values showed statistically significant differences. Conclusion: The IIT group showed a better pink and white esthetic outcome than the DIT group in a short observing period, and they can get comparable implant survival.

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Microbial Differences in Dental Plaque of Deciduous Teeth between Caries-active Children and Caries-free Children.

FENG Yan, YAN Heng, XUE Kai-jia, LU You-guang.

2016, 32(1): 55-58. DOI: 10.11839/kqyxzh.2016.01.014

Abstract ( 295 )

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Objevtive: To discuss the microbial differences in dental plaque of deciduous teeth between caries-active children and caries-free children. Methods: Three caries-active children and three caries-free children were selected according to WHO standard. Three specimens of plaque (from labial-buccal surfaces, lingual surfaces and caries lesions) were taken from each caries-active children and two specimens of plaque (from labial-buccal surfaces and lingual surfaces) were taken from each caries-free children. DNA of the whole genome was extracted. PCR amplification of the V1-V2 region of 16SrDNA was conducted and the gene library was constructed. The method of 454 pyrosequencing was used. The resultant sequences were classified by RDP classifier and the statistic analysis was conducted. Results: A total of 324,262 gene sequences were obtained from 15 specimens, there were 21617 gene sequences for each specimen averagely and the average length was 268 bps. In caries-active children, 5 bacterial strains were found significantly different among the different locations (P<0.05). They were Streptococcus, Propionibacterium, Corynebacterium, Leptotrichia and Lactobacillus. There were 5 bacterial strains significantly different between caries-active children and caries-free children (P<0.05), including Streptococcus, Filifactor, Solobacterium, Thermus and Brevibacillus. Conclusion: The microorganism detection rate in dental plaque is different between caries-active and caries-free children.

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Analysis of Canal Morphology of Mandibular First Premolar in Uighur Population of Xinjiang.

GUO Jing, LIANG Xue-ping.

2016, 32(1): 59-62. DOI: 10.11839/kqyxzh.2016.01.015

Abstract ( 221 )

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Objective: To investigate the canal morphology of bilateral mandibular first premolars in Uighur population of Xinjiang using cone-beam computed tomography (CBCT). Methods: A total of 200 CBCT images of mandibular first premolars were obtained from 100 patients. Tooth position, root canal morphology, symmetry of bilateral mandibular first premolars, and the distance between the 2 root canal orifices of mandibular first premolars were investigated. The classifications of the root canal types were according to Vertucci's type. Results: 1.5% (n=3) of the teeth had a C-shaped root canal system, 76.5% (n=153) had 1 root canal, and 22% (n=44) had 2 root canals from type II to type V. The distance between the 2 root canal orifices of mandibular first premolars was approximately 1-3 mm, and the symmetry in all types of canal classification of mandibular first premolar was 85%. Conclusion: CBCT can provide accurate information of canal system. More than 20% of mandibular first premolars had complex root canal systems showing multi-canal or double root canals.

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Study on the Classification of Clinical Crown of Youth Maxillary Central Incisors.

LIN Zi-qi, FANG Hui-min, ZHANG Wei, CAO Yang, YU Qing.

2016, 32(1): 63-66. DOI: 10.11839/kqyxzh.2016.01.016

Abstract ( 194 )

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Objective: To evaluate the applicability of the Tufts University Clinical Crown Classification Index to the youth maxillary central incisors in Nanjing, China, and compare the contact area positions of each classification. Methods: Digital photography was used to get 114 maxillary central incisor samples. According to the Index, all samples were divided into 3 groups: triangle, round and square types. Then the crown length (CL), width (CW), contact surface (CS), gingival angle (GA) were tested by Digimizer. Results: There was significant difference among 3 groups with respect to CS/CL ratios, CW/CL ratios and GA values. Conclusion: The Tufts University Clinical Crown Classification Index is applicable to the youth maxillary central incisors in Nanjing, China. The contact area of triangle group is lower than the other two groups.

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Investigation of Patients’ Satisfactory Degree of Effects of Beyond Cold Light Whitening.

XU Ying-xin.

2016, 32(1): 67-70. DOI: 10.11839/kqyxzh.2016.01.017

Abstract ( 213 )

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Objective: To investigate the patients’ satisfactory degree about their treatment, to compare the clinical effects of Beyond Cold Light Whitening on tetracycline teeth, dental fluorosis and yellow teeth, and to evaluate the usefulness of Vitapan Classical Shade Guide in judging effects of tooth whitening. Methods: 57 patients who underwent Beyond Cold Light Whitening treatment were collected in June 2008 to January 2014. The treatment effects were evaluated by Vitapan Classical Shade Guide and the patients’ satisfactory degree were evaluated by Patients Self-Assessment Scale. Results: The mean of color gradation change by Vitapan Classical Shade Guide was 0.85±1.91 in tetracycline teeth, 3.69±2.91 in dental fluorosis, 4.61±2.35 in yellow teeth, and the mean of scores by Patients Self-Assessment Scale was 6.69±1.03 in tetracycline teeth, 7.42±1.42 in dental fluorosis, and 8.00±1.24 in yellow teeth. Both of them in tetracycline teeth were significantly lower than dental fluorosis and yellow teeth. Conclusion: The whitening effects of tetracycline teeth by Beyond Cold Light Whitening were limited. The traditional way of evaluating the clinical effects of Beyond Cold Light Whitening by Vitapan Classical Shade Guide should be improved.

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Association between the Developmenal Stages of Mandibular Third Molars and Retromolar Space.

FAN Ya-ru, ZHAO Hao-ran, LIU Hai-xia, LUO Hui.

2016, 32(1): 71-74. DOI: 10.11839/kqyxzh.2016.01.018

Abstract ( 266 )

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Objective: To assess the association between the developmental stages of mandibular third molars and the space distal to the permanent molars (retromolar space), and to predict the growth potential of retromolar space and provide diagnostic basis for clinical treatment. Methods: The materials included pretreatment lateral cephalographs of 129 orthodontic patients (62 males, 67 females; 8-18 years old) from Urumqi Stomatological Hospital. The molar formation stage was evaluated by the method of Nolla. The retromolar space was measured from the most concave point of the anterior border of the ramus to the distal surface of the first molar by CBCT. Statistical analyses included t-tests, the Pearson product moment and the linear regression equations. Results: Between gender groups the retromolar space was different, and the age and formation stages of the mandibular third molars had no statistical difference. The correlation between retromolar space and developmental stage was high (r=0.860,P<0.001). Conclusion: We can predict the mandibular retromolar space by estimating the dental age of mandibular third molars, and provide reference for clinical diagnosis and treatment planning

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Application of Modified Vascularized Interpositional Periosteal-connective Tissue Technique in Immediate Implantation in the Maxillary Anterior Region.

WANG Qun, HU Fang-fang, GAN Chao-bing, LU Yu, KANG Lin, WU Hai-zhen.

2016, 32(1): 75-78. DOI: 10.11839/kqyxzh.2016.01.019

Abstract ( 236 )

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Objective: To explore the clinical efficacy of modified vascularized interpositional periosteal-connective tissue technique in the esthetic dental immediate implantation. Methods: 30 traumatic teeth that could not be maintained in esthetic zone were included in this study. A modified vascularized interpositional periosteal-connective tissue technique was applicated for the augmentation of peri-implant mucosa and closure of the immediate implantation wound. Clinical outcomes were evaluated. Results: The clinical survival rate of the flaps was 96.67%. The average Pink Esthetic Score was 12.20 immediately after final restorations. 6 months later, mesial papilla, distal papilla, soft-tissue contour and PES score were significantly improved. However, no significant difference was found between 12 months and 6 months after final restoration. Conclusion: The application of modified vascularized interpositional periosteal-connective tissue technique during immediate implantation in the maxillary anterior region can contribute to close the wound and gain a good pink esthetic outcome.

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Factors Affecting the Success Rate of Miniscrewsas Orthodontic Anchorage.

CAO Xiao-qing, ZHANG Li, LIU Long-kun.

2016, 32(1): 79-82. DOI: 10.11839/kqyxzh.2016.01.020

Abstract ( 234 )

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Objective: To evaluate various factors that influencing the success rate of miniscrews used as orthodontic anchorage. Methods: 114 patients were included with a total of 253 miniscrews. Potential confounding variables examined were gender, age, vertical (FMA) and sagittal (ANB) skeletal facial pattern, site of placement (labial and buccal, palatal and retromandibular triangle), jaw of placement (maxilla and mandible), soft tissue type, oral hygiene, diameter and length of miniscrews, insertion method (predrilled or drill-free), angle of placement, onset and strength of force application and clinical purpose. The correlations between success rate and overall variables were investigated by logistic regression analysis, and the effect of each variable on the success rate was utilized by variance analysis. Results: The overall success rate was 88.54% with an average loading period of 9.5 months in successful cases. Age, oral hygiene, vertical skeletal facial pattern (FMA) and general placement sites (maxilla and mandibular) presented significant differences in success rates both by logistic regression analysis and variance analysis (P<0.05). Conclusion: To minimize the failure of miniscrews, proper oral hygiene instruction and effective supervision should be given for patients, especially the young (<12 years) high-angle patients with miniscrews placed in the mandible.

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Effect of Streptococcus Mutans Invasion on the Aging of Four Kinds of Composite Resin Bonding Interface.

WANG Nan-yan.

2016, 32(1): 83-86. DOI: 10.11839/kqyxzh.2016.01.021

Abstract ( 356 )

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Objective: To assess the effect of Streptococcus mutans invasion on the aging of four kinds of composite resin bonding interface. Methods: Two kinds of bulk-fill composite resins and two kinds of conventional composite resins were selected and immersed into bacterium solution for 14 days. After the specimen was subjected to ultrasonic cleaning, the bonding surface cracks were observed under a stereoscopic microscope, and the percentage of cracks areas were evaluated. Two samples in each group were then picked and sprayed using ion sputtering equipment. After fixed with conductive adhesives, the samples were observed under a scanning electron microscope. Results: Streptococcus mutans invasion led to different destruction of these composite resins bonding interface with no significant difference among the groups. According to the damage classification, SDR had the highest damage average, and the damage average of Z350 was slightly higher than that of P60 and Tetric N-Ceram Bulk Fill. The damage was located between the bonding surface and the dentin of P60 and Tetric N-Ceram Bulk Fill, while between the adhesive surface and the resin of Z350 and SDR. Conclusion: Streptococcus mutans can damage the surface and bonding interface of composite resin in a short time. Different resin components exhibited different failure modes, but showing no significant difference in the extent of damage.

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Expression and Significance of P53 and Ki67 in Oral Squamous Cell Carcinoma and Relation to the Prognosis of Patients.

Palati·Tuerxun, Reziya·Aini, Muhetaer·Huojia.

2016, 32(1): 87-90. DOI: 10.11839/kqyxzh.2016.01.022

Abstract ( 243 )

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Objective: To investigate the expression of p53 and ki67 proteins, and their relations to prognosis of patients in oral squamous cell carcinoma (OSCC). Methods: A retrospective study including 132 patients with OSCC was performed, and the follow-up time was at least 5 years. The expression levels of p53 and ki67 were detected by immunohistochemistry and analyzed for the correlation with pathological features and prognosis of patients in OSCC. Results: The expression levels of p53 and ki67 correlated with cell differentiation, TNM stages and tumor site in OSCC (P＜0.05). In addition, the expression of p53 was an independent prognostic parameter in patients with OSCC (P＜0.05), but ki67 expression was not. Conclusion: The expression level of p53 is an effective biomarker and an independent prognostic parameter in oral squamous cell carcinoma

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Effects of Modified Marginal Resection of Mandible Remaining Its Continuity with Cmbined Radical Neck Dissection in the Surgical Management of Tongue Squamous Cell Carcinoma

ZHAO Ze-liang, YE Mao-chang, LI Rong-xin, WANG Lai-ping, ZHOU Yu, ZHU Wei-zheng, WANG Yi, PENG Hui

2016, 32(1): 91-94. DOI: 10.11839/kqyxzh.2016.01.023

Abstract ( 211 )

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Objective: To investigate the feasibility of modified marginal resection of mandible remaining its continuity with combined radical neck dissection in the surgical management of tongue squamous cell carcinoma. Method: Modified marginal resection of mandible remaining its continuity with combined radical neck dissection was used in 137 cases of tongue squamous cell carcinoma. In the modification group, slice cut and selective grinding of bone between inner inferior border of mandible and mucosa of floor of mouth were added. Selective/radical neck dissection was applied. 30-month follow-up was conducted at least. Results: The median follow-up time was 42 months. Three- or five-year survival rate was 74.45% (101/137) and 70.80% (97/137), respectively. Mastication, swallowing, speech and appearance of all the cases were significantly improved. The curative effect was satisfactory. Conclusion: Modified marginal resection of mandible remaining its continuity with combined radical neck dissection is feasible in the treatment of tongue squamous cell carcinoma.

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Non-extraction Treatment for Severe Dentition Crowding: A Case Report

MU Lan, ZHANG Xiao-bo,XU Geng-chi, GE Zhen-lin.

2016, 32(1): 95-95. DOI: 10.11839/kqyxzh.2016.01.02

Abstract ( 255 )

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Objective: To report a case of severe dentition crowding with non-extraction treatment. Methods: The patient was aligned dentition by molar propeller and MBT straight wire appliance. Results: The patient face type was maintained and the upper and lower dentition were aligned after treatment. Conclusion: Molar propeller can effectively treat severe dentition crowding

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