Journal of Oral Science Research ›› 2016, Vol. 32 ›› Issue (1): 8-11.DOI: 10.11839/kqyxzh.2016.01.00

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Alternative Splicing of SPAG5 Exon3 is Regulated by SRSF3 in Oral Squamous Cell Carcinoma.

LIU Pei-qi, SHI Li-ming, JIA Rong.   

  1. The State Key Laboratory Breeding Base of Basic Science of Stomatology & Key Laboratory of Oral Biomedicine Ministry of Education, School of Stomatology, Wuhan University, Wuhan 430079, China
  • Received:2015-07-29 Online:2016-01-28 Published:2016-03-21

Abstract: Objective: To study the effect of SRSF3 on alternative splicing of SPAG5 exon3 in oral squamous cell carcinoma (OSCC). Methods: SRSF3 was knocked down using siRNA in OSCC CAL27 cells. Alternative splicing of SPAG5 exon3 was analyzed by RT-PCR. Results: Knockdown of SRSF3 inhibited the expression of full length SPAG5 and increased short spliced product of SPAG5 in CAL27 cells. Conclusion: SRSF3 could down-regulate the expression of SPAG5 protein and interfere with mitosis in OSCC.

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