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Animal Study on the Ultrasound Accelerated Orthodontic Tooth Movement. LUAN Li-yang, JIANG Jiu-hui, LI Cui-ying, WANG Hai-chen, GAO Long-hua. 2016, 32(2): 109-112.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.001 Abstract ( 435 )   PDF (1696KB) ( 469 )   Objective: To observe whether ultrasound could accelerate tooth movement in a rat orthodontic model. Methods: In this study, 30 rats were randomly divided into 2 groups, with 5 rats at each time point. Orthodontic appliances were placed between the maxillary first molars and central incisors. The rats were randomly divided into 2 groups: group 1 was normal treatment and group 2 was ultrasound treatment. The rats were sacrificed at different time points (t=7, 14, 28 days), the distances of tooth movement were measured and histologically observed. Results: The ultrasound significantly increased orthodontics tooth movement distances at day 7, 14, 28 compared with conventional orthodontic group. Histological observation demonstrated that the count of osteoclasts in the experimental group was significantly more than that in the conventional orthodontic group. Via Masson staining, new bone formation was found in both groups at the 7th day; after 14 days, there were more new bone formation in the group 1; 28 days later, more new bone formation was found in the group 2. Conclusion: The results suggest that the ultrasound can significantly increase the osteoclastogenesis around the moving tooth. This effect may induce the accelerated tooth movement. References | Related Articles | Metrics

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Effects of iRoot BP Plus on Inflammatory Bone Destruction in Mice. LIU Shan, ZHANG Jie, ZHU Ling-xin, PENG Bin. 2016, 32(2): 113-116.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.002 Abstract ( 481 )   PDF (1969KB) ( 296 )   Objective: To compare the effects of new oral bioceramic material iRoot BP Plus and mineral trioxide aggregate (MTA) on LPS-induced bone destruction in mice. Methods: 24 BABL/C mice were randomly equally divided into four groups injected by 10 mg/kg LPS with or without the material extracts or culture medium alone once a day. After treatment for 7 days, mice were sacrificed and calvarias were detected by micro-computed tomography scanning, histologic analysis and double immunofluorescence staining. Results: iRoot BP Plus and MTA significantly alleviated bone destruction in mice calvarias, inhibited the formation and resorption capacity of osteoclasts, and markedly downregulated the expression level of osteoclastogenic cathepsin K. Conclusion: iRoot BP Plus had inhibitory effects on inflammatory bone destruction in mice. References | Related Articles | Metrics

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Impact of DAPT on the Proliferative Activity of Cementoblast. HU Zhi-ai, PAN Jie, LI Yu-yu, ZHOU Ze-yuan, HUANG Li, ZOU Shu-juan. 2016, 32(2): 117-121.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.003 Abstract ( 293 )   PDF (1352KB) ( 234 )   Objective: To clarify whether DAPT (a γ-secretase inhibitor) could inhibit the Notch signaling pathway in the cementoblast OCCM30. To investigate the impact of DAPT on the proliferation of the cementoblast and the mechanism behind. Methods: The OCCM30 cells were cultured with γ-secretase inhibitor DAPT. On day 2, 4 and 6, RT-PCR was conducted to detect the expression of the Notch signaling pathway genes (Hes-1 and Hey-1) and cell cycle related genes (Cyclin D and Cyclin E) and flow cytometry was used to investigate the impact of DAPT on the cell cycle of OCCM30. On day 8, the CCK-8 was carried out for the detection of the proliferative activity of the OCCM30. Results: Compared to the control group, the expression level of Hes-1, Hey-1, Cyclin D and Cycin E in the experimental groups decreased (P<0.05) while the G1/G0 cell proportion increased (P<0.05). From day 2 to day 8, the proliferative activity of the cells in the experimental groups decreased significantly (P<0.05). The proliferative activity of the OCCM30 in the higher DAPT concentration group was lower than that in the lower DAPT concentration group, both of which were significantly lower than the control group (P<0.05).Conclusion: DAPT effectively inhibited the proliferative activity of the OCCM30. The mechanism behind that could be the inhibitory effect of the DAPT on the Notch signaling pathway, down-regulating the expression of the cell-cycle related genes. References | Related Articles | Metrics

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Expression of CD146 in Postnatal Human Dental Pulp Stem Cells YANG Xue-chao, LV Xiao-shuai. 2016, 32(2): 122-125.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.004 Abstract ( 322 )   PDF (1238KB) ( 287 )   Objective: To study the expression of CD146 in postnatal human dental pulp stem cells (DPSCs) and during cell differentiation. Methods: DPSCs were cultured in vitro and determined the expression of CD146 through immunofluorescence and fluorescence activated cell sorting (FACS). DPSCs were cultured in the condition of mineralized inducement. During the inducement, CD146 was detected through RT-PCR and immunofluorescence. Results: There was positive expression of CD146 in DPSCs. The results of FACS showed that the positive rate of CD146 was 58.78% in DPSCs. During odontogenetic differentiation, confirmed by DSP, the expression of CD146 was down-regulated. Conclusion: There was relatively specific expression of CD146 in DPSCs. CD146 may be a specific marker of DPSCs. References | Related Articles | Metrics

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Study on the Methylation and Mutation of CDH1 in the Process of SD Rats Tongue Carcinogenesis. WANG Jun-lian, ZHANG Xu, MAO Liang, TAN Hong, LIAO Peng-cheng, NIE Min-hai. 2016, 32(2): 126-130.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.005 Abstract ( 324 )   PDF (1215KB) ( 265 )   Objective: To detect the CDH1 gene methylation and mutation in exon at different pathological stages of tongue mucosa carcinogenesis. Methods: Seventy-five SD rats were administered by 0.004% 4-nitroquinoline-1-oxide (4NQO) in drinking water, and 15 SD rats were kept in the same conditions without 4NQO as negative control group. After 10, 14, 18, 22 and 24 weeks, the rats were euthanatized, and the tumorsin the tongue mucosa were collected for histological examination and DNA extraction. Then, methylation specific polymerase chain reaction (MS-PCR) was performed to detect the levels of CDH1 promoter methylation, and PCR amplification of CDH1 exons 1-16 was performed to detect CDH1 exons mutations in the various stages of tongue squamous cell carcinoma (TSCC). Results: There were no CDH1 promoter methylation specific products detected in different stages of SD rats TSCC. In sequenced exons 1-16 of all samples, only exon 13 mutation was detected at the site of mRNA 2106 (G→T). Conclusion: CDH1 mutations in exons may be associated with the development of SD rats TSCC which induced by 4NQO, but CDH1 promoter methylation was not. References | Related Articles | Metrics

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Observation on Primary Cilia of Human Periodontal Ligament Cells and Periodontal Tissue. YIN Feng-ying, SUN Hui-fang, WANG min, ZHANG Fan, CHENG Xiang-rong, XIA Hai-bin 2016, 32(2): 131-133.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.006 Abstract ( 368 )   PDF (1220KB) ( 313 )   Objective: To observe primary cilia in periodontal tissue and human periodontal ligament cells (hPDLCs) cultured in vitro. Methods: The ligaments of human and mouse were cut from the middle of roots and made into frozen and paraffin section respectively. hPDLCs were cultured in vitro. Then acet-α-tubulin antibody was used to detect the primary cilia of periodontal tissue and cells. Results: The signal of primary cilia was short rod near to the nuclei in periodontal ligament and primary hPDLCs. The length of most primary cilia was about 2-3 μm. Conclusion: Both periodontal ligament and primary hPDLCs have the primary cilia. References | Related Articles | Metrics

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Effects of Combined Chemotherapy of SB225002 and Cisplatin on OSCC Cells In Vitro WANG Xian-ling, LIU Jian-wei, QIAN Yong. 2016, 32(2): 134-138.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.007 Abstract ( 300 )   PDF (2593KB) ( 190 )   Objective: To investigate the effects of SB225002 and cisplatin on oral squamous cell carcinoma (OSCC) cells, and meanwhile explore the possible mechanism. Methods: The OSCC cell proliferation, apoptosis and cell cycle were detected by MTT assay and flow cytometry after treated with SB225002 and cisplatin alone or in combination, and then correlated analysis was performed. Results: SB225002 could significantly inhibit the proliferation, promote apoptosis and induce G2/M arrest of OSCC cells in a concentration-dependent manner. Moreover, combined chemotherapy of SB225002 and cisplatin showed stronger effects, and the differences were statistically significant (P<0.05). Conclusion: There is synergistic effect between SB225002 and cisplatin on the anti-tumor activity in OSCC cells. References | Related Articles | Metrics

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Expression of Transcription Factor XBP1 in Oral Squamous Cell Carcinoma. JIANG Fang-yan, WANG Yu, CHEN Ji-rong, SUN Ya-nan, CHEN Xin-ming, ZHANG Jia-li. 2016, 32(2): 139-142.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.008 Abstract ( 404 )   PDF (2077KB) ( 427 )   Objective: To detect the expression and explore the role of X box binding protein 1 (XBP1) in oral squamous cell carcinoma (OSCC). Methods: Real-time PCR was performed to detect the expression of X box binding protein 1 splicing (XBP1-s) in 50 cases of fresh primary OSCC samples and the corresponding matched epithelial tissues. The tissue microarray technique and immunohistochemistry were used to detect XBP1 protein expression in 100 cases of OSCC samples, and each case included the adjacent normal epithelial, primary tumor center area, invasive front and lymph node metastasis. Results: The relative expression of XBP1-s mRNA with more than 2-fold increase in OSCC tissue was closely related with lymph node metastasis (P＜0.05). Immunohistochemistry staining showed that the expression levels of XBP1 protein in the invasive front and lymph node metastasis were higher compare with that in the epithelial and tumor center (P＜0.05). Additionally, XBP1 protein expression in lymph node metastasis was significantly higher than that in invasive front (P＜0.05). Moreover, XBP1 protein expression was significantly correlated with pathological grade of OSCC tissues (P＜0.05). Conclusion: XBP1 might play an important role in OSCC invasion and promote tumor metastasis. References | Related Articles | Metrics

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Effect of Porphyromonas Gingivalis Infection on Human Trophoblast Cells Proliferation and Apoptosis. LI Lei-tao, REN Hong-yu, LI Yu-hong, JIANG Han, DU Min-quan. 2016, 32(2): 143-146.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.009 Abstract ( 308 )   PDF (1453KB) ( 249 )   Objective: To observe the effect of Porphyromonas gingivalis ATCC 33277 (P.g ATCC 33277) infection on human trophoblast cell line (HTR-8 cells) proliferation and apoptosis in vitro. Methods: HTR-8 cells were infected with P.g at a multiplicity of infection (MOI). Non-infected HTR-8 cells were used as the control. After Infection, the Cell Counting Kit(cck-8)was employed to assay the proliferation of HTR-8 cells. The apoptosis of HTR-8 cells were detected by mitochondrial membrane potential assay kit with JC-1 apoptosis detection kit. Result: At an MOI of 10, no inhibition on HTR-8 cells proliferation was observed following 24-72 h of P.g infection in contrast to the non-infected control (P>0.05). However, P.g significantly inhibited the proliferation of HTR-8 cells at an MOI of 100 or 200 at 24 h (P<0.01). In addition, cells infected with P.g at an MOI of 200 had a significantly lower lever of proliferation than cells infected with P.g at an MOI of 100 at 48 h (P<0.05) and 72 h (P<0.01). Furthermore, following 24 h of infection, P.g at an MOI of 200 significantly increased the rate of apoptosis in HTR-8 cells (P<0.01). A significant higher apoptosis rate in HTR-8 cells was also observed at an MOI of 100 after 48 h of infection (P<0.01). The rate of apoptosis was comparable between the control cells and the infected cells at an MOI of 10 at 24 h or 48 h (P>0.05). Conclusion: Our results demonstrate in vitro that P.g ATCC 33277 infection is able to suppress the proliferation of HTR-8 cells, and in the meantime, enhance the apoptosis of the cells. These results suggest that P.g-induced proliferation and apoptosis imbalance of trophoblast cells is involved in periodontal infection-associated preterm low birth weight. References | Related Articles | Metrics

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Application of Cyst Plug with a Lip Bow and Ring Fixed in Drainage after Marsupialization of Large Mandibular Cystic Lesions. Feng Xue-feng, Xiao Di, Wang Ru. 2016, 32(2): 147-149.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.010 Abstract ( 252 )   PDF (1380KB) ( 448 )   Objective: To investigate the clinical effect of a new cyst plug. Methods: Seven patients with large mandibular cystic lesions were treated and fixed with the cyst plugs with lip bow and rings after marsupialization. Results: All mandibular cystic lesions were reduced in the volume, which was attributable to the maintained and unobstructed open of the cysts by the new drainage device in all patients.Conclusion: The application of cyst plug with a lip bow and rings might be a suitable clinical drainage treatment for large mandibular cystic lesions. References | Related Articles | Metrics

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Effect of Periodontal Infection on APP and IL-6 Levels in Patients with Cerebral Infarction. ZHANG Zheng, ZHANG Li, ZHENG You-li, MA Ning. 2016, 32(2): 150-153.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.011 Abstract ( 236 )   PDF (1009KB) ( 205 )   Objective: To study the changes of acute phase protein (APP) and interleukin-6 (IL-6) levels in patients with cerebral infarction and effect of systemic antibody response of periodontal infection on APP and IL-6 levels.Methods: Eighty-eight patients with acute cerebral infarction for the first time and forty healthy subjects were included in this study. The subjects were grouped into Pg-IgG antibody positive [Pg-IgG (+) ] group and Pg-IgG antibody negative [Pg-IgG(-)] group according to Porphyromonas gingivalis IgG (P.g-IgG) levels. Serum high-sensitivity C-reactive protein (Hs-CRP), fibrinogen (Fg) and IL-6 levels were detected by enzyme-linked immune sorbent assay method and automatic blood coagulation analyzer. Results: Hs-CRP, Fg and IL-6 were significantly higher in patients with acute cerebral infarction than healthy subjects. Hs-CRP and IL-6 were significantly higher in Pg-IgG (+) group than that in Pg-IgG (-) group. Hs-CRP and IL-6 were positively correlated with Pg-IgG. Conclusion: Periodontal Porphyromonas gingivalis infection may increase cerebral infarction risk by promoting the levels of Hs-CRP and IL-6. References | Related Articles | Metrics

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Clinical Study on Cervical Lymph Nodes Metastasis of Oral Squamous Cell Carcinoma. HAN Ying, SU Tong, LIN Lu, QIAN Yun-mei, XIONG Hao-feng 2016, 32(2): 154-157.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.012 Abstract ( 272 )   PDF (763KB) ( 338 )   Objective: To evaluate the clinical pathological factors affecting contralateral neck lymph node metastasis in oral squamous cell carcinoma (OSCC), and provide clinical evidence of prophylactic contralateral neck dissection for surgeons. Methods: A total of 238 OSCC cases were retrospectively reviewed from Jun. 2010 to Dec. 2012 in the Department of Oral and Maxillofacial Surgery, Xiangya Hospital of Central South University. The impact of multiple clinicopathologic factors, including age, sex, course of disease, primary site, T-stage, type of neck dissection, ipsilateral metastatic lymph nodes and histopathological grading were assessed by logistic regression analysis. Results: Univariate logistic regression analysis showed that contralateral neck lymph node metastasis was significantly increased in the OSCC patients with T3/T4 stages, moderately and poorly cell differentiation, or ipsilateral neck lymph node metastasis. Multivariable logistic regression analysis showed that only pathology grade was high risk factor for contralateral neck lymph node metastasis of OSCC. Conclusion: Prophylactic contralateral neck dissection should be considered if patients in the middle stage of the tumor had more than two signs as followed: T3/T4 tumor stage, ipsilateral clinically lymph node-positive, and poorly differentiated. References | Related Articles | Metrics

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Marginal Microleakage of Different Filling Methods in Restoring Wedged-shaped Erosion. PENG Zao-xia, LI Ning, CHEN Xi, DU An-qing, MI Cong-bo, LIANG Xue-ping. 2016, 32(2): 158-160.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.013 Abstract ( 328 )   PDF (823KB) ( 455 )   Objective: To compare the marginal microleakage of fillings for wedged-shaped erosion restoration using Hawe'S tooth neck matrix band or normal plugger. Methods: Standardized Class V cavities (3mm 2mm 2mm) were prepared at the cemento-enamel junction on buccal surface of 40 recently extracted human premolars. The preparations were randomly assigned to two groups (20 for each group) according to the instrument used (group A using normal plugger and group B using Hawe'S matrix), and then restored with 3M (Filtek Z350) filling composite resin. After thermal circulation and soaked for 24 hours in 2% methyl blue solution, the specimens were cut through the midline of the restoration buccolingually and longitudinally. The microleakage at the occlusal margin and the gingival margin were observed under a stereomicroscope and the leakage depth was measured with Image-pro plus 6.0 software package.Results: Microleakage was observed in two groups. The microleakage depth of group A was (0.641±0.370) mm, and the microleakage depth of group B was (0.395±0.462)mm . Significant difference was observed between group A and group B (P<0.05). Conclusion: Hawe'S tooth neck matrix band is better than normal plugger in reducing the microleakage of fillings for wedged-shaped erosion restoration, which is worthy of wide clinic application. References | Related Articles | Metrics

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Clinical and Radiographic Evaluations of Lateral Sinus Lift without Grafts. CEHN Xi-nan, LIN Yi, HE Tian-rong. 2016, 32(2): 161-164.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.014 Abstract ( 360 )   PDF (1136KB) ( 410 )   Objective: To evaluate the clinical outcome and radiographic new bone formation of simultaneous lateral sinus lift without grafts. Methods: Twenty posterior partially edentulous patients with residual bone height (RBH) between 4-5mm were chosen to receive the lateral sinus lift. Patients were randomly assigned into two groups. Trial group: 10 patients received 12 implants using lateral sinus lift technique without grafts. Control group: 10 patients received 11 implants using lateral sinus lift technique with hydroxyapatite graft. All patients were followed regularly after the surgery up to 12 months. The success rate of the implants, the radio examination of the bone height and implant stability quotient (ISQ) were checked during the revisits.Results: All implants were stable during the study period. In the trial group, the mean values of the mesial aspect increased from (4.50±0.21) mm to 6.88±0.85 mm at 3 months and to (7.42±0.66) mm at 6 months, stayed stable at (7.55±0.55) mm when reached 12 months. The same pattern was observed in the distal side. In the control group, RBH of the mesial aspect decreased from (11.29±0.64) mm to (10.11±0.62) mm at 3 months and to (9.51±0.39) mm at 6 months, stayed stable at 9.59±0.39 mm when reached 12 months. The similar changing pattern was discovered in the distal aspect. The differences between two groups were significant (P<0.05). The ISQ value increased from (53.92±7.31) to (64.77±9.22) at 6 months in the trial group and from (56.23±8.05) to (67.29±8.34) in the control group (P<0.05). Conclusion: The short-term clinical result of lateral sinus lift without grafts was satisfied and some extent of endo-sinus bone gain could be observed. References | Related Articles | Metrics

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Effect of Initial Periodontal Therapy on the Serum OPN and IL-17 Levels in Subjects with Oral lichen Planus. ZHANG Hong-chi, LIU Li-sha, ZHANG Rui-min. 2016, 32(2): 165-167.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.015 Abstract ( 255 )   PDF (747KB) ( 269 )   Objective: To investigate the effect of periodontal basic treatment on peripheral blood levels of OPN and IL-17 in oral lichen planus (OLP) patients. Methods: Sixty OLP with periodontitis in the second affiliated hospital of Harbin medical university were randomly divided into two groups. The experimental group (30 cases) received periodontal treatment combined with medications and the control group (30 cases) received drug therapy only. The serum levels of OPN and IL-17 were determined before and after periodontal treatment by ELISA. Results: The serum levels of OPN and IL-17 after treatment were significantly lower than those before treatment in two groups. The experimental group showed significantly lower OPN and IL-17 levels than the control group after treatment. Conclusion: Periodontal basic treatment was beneficial to the OLP therapy. References | Related Articles | Metrics

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Effect of Storage Condition on the Osteoblasts Activity on Sandblasted and Acid-etched Titanium Surfaces. CHEN Xue-lin, XIAO Yue. 2016, 32(2): 168-171.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.016 Abstract ( 309 )   PDF (852KB) ( 215 )   Objective: To investigate the effects of different storage methods on adhesion and proliferation rate of osteoblasts on the surface of titanium implant. Methods: The titanium plates were modified with sandblasted and acid-etching technology and divided into three groups according to the storage methods. Group A was in the airtight container, group B was in the steaming water (ddH2O), and group C was in the sodium hydroxide (NaOH) solution for 4 weeks. Adhesion and proliferation of osteoblasts at different time points on the surface of titanium were evaluated by MTT assay, alkaline phosphatase activity assay, and laser scanning confocal microscope. Results: After 1 hour and 3 hours culture, the number of cells on group B and group C surface were higher than that on group A (P<0.05). After 1, 4, 7 days culture, the number of cells on group C was higher than those on other two groups (P<0.05). Under laser scanning confocal microscope, osteoblasts were found to spread better on group C surface. After 7 days culture, alkaline phosphatase activity of cells on group C was higher than those on group B and group A (P<0.05). Conclusion: The titanium plates preserved in the liquid was beneficial to maintain the biological activity. Sodium hydroxide solution might be the optimal way for implant storage. References | Related Articles | Metrics

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Relationship between Wnt5a and Bone Resorption in Chronic Apical Periodontitis. XU Li-ya, MA Nan, QIU Li-hong, YU Ya-qiong, Yu Jing-tao, Xue Ming 2016, 32(2): 172-176.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.017 Abstract ( 240 )   PDF (988KB) ( 193 )   Objective: To detect the expression of Wnt5a mRNA in lesions of chronic apical periodontitis. Methods: 18 patients of chronic apical periodontitis were divided into group A (d≥6 mm) and group B (6 mm＞d≥3 mm) according to the scope of the root lesions. 21 healthy teeth extracted for orthodontic reasons were selected as the control group. Expression of Wnt5a mRNA was analyzed with RT-PCR (Real-Time PCR) to identify its relationship with the scope of the lesion. 10 patients of chronic apical periodontitis and 5 healthy controls were chosen for immunohistochemistry. All statistical analysis was performed using the SPSS 13.0 statistical package. Results: Wnt5a mRNA expression in patients increased significantly than that in normal controls (P<0.05). Compared with control group, group A had a significant increase in Wnt5a mRNA expression (P<0.01). The difference between group A and group B was also significant (P<0.01). While there was no significant difference between group B and the control group (P＞0.05). According to the results of immunohistochemistry, Wnt5a expression in group A (P＜0.01) and group B (P＜0.05) was significantly higher than that in normal controls. Wnt5a expression in group A was higher than that in group B (P＜0.05). Conclusion: Wnt5a expression is associated with the bone resorption in chronic apical periodontitis. References | Related Articles | Metrics

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Correlation of Salivary Flow Rate and Xerostomia in Diabetes Mellitus Patients. YU Jie, ZHANG Gong-jie, CHEN Song-jun, ZHENG Min, LI Li. 2016, 32(2): 177-180.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.018 Abstract ( 319 )   PDF (762KB) ( 398 )   Objective: To evaluate xerostomia, salivary flow rate in diabetes mellitus patients and investigate it's relevance to history and metabolism. Methods: Type 1 diabetes mellitus (n=21) and type 2 diabetes mellitus (n=54) were selected, while healthy people with similar age and gender were chosen as control groups. Resting salivary flow rate was measured and xerostomia was evaluated by xerostomia questionnaire (XQ) in four groups. Results: Compared to control group, salivary flow rates of diabetes patients were decreased, but the decline degree in type 2 diabetes mellitus was small (P>0.05). Two diabetic mellitus groups complained xerostomia. OX scores were higher than those of control group (P<0.05). Salivary flow rate of type 2 diabetes mellitus was lower than type 1 diabetes mellitus and OX score was higher than that of type 1 diabetes mellitus (P>0.05). Sectionalized by HbA1C≤6% and history≤3 years (type 2 diabetes mellitus≤5 years), there was no difference in salivary flow rate and xerostomia between sectionalizations (P>0.05). Conclusion: Diabetes patients complain xerostomia obviously and their salivary flow rates decrease mildly compared to normal population. More attention should be paid in oral care for diabetes mellitus patients. References | Related Articles | Metrics

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Radiographic Features of Mandibular Cone Beam Computed Tomography Film in Patients with Idiopathic Condylar Resorption. WANG Yuan, ZHANG Juan, LI Ze-kui, LI Hong-fa. 2016, 32(2): 181-184.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.019 Abstract ( 278 )   PDF (915KB) ( 308 )   Objective: To analyze the radiographic features of mandibular cone beam computed tomography (CBCT) films in patients with idiopathic condylar resorption (ICR). Methods: Forty-one ICR patients on their first visit to the Department of Orthodontics were selected as the experiment group, and 41 healthy volunteers were served as the control group. Mandibular indicators on CBCT were collected by Invivo5 software and the statistical differences were analyzed by SPSS 17.0. Results: Indicators that showed significant differences between the experimental group and control group included SNB, NP-FH, S-Go, Co-Go, S-Go/N-Me, H, ACo-PCo, PCo-Co , angle of anterior slope, S-Ar-Go and Co-Go-Me (P<0.05), while those showed no significant differences between two groups included N-Me, Go-Me, S-PCo, ACo-Co and angle of posterior slope(P>0.05). Conclusion: Bone resorption in ICR mainly occurred in the top and anterior slope of the condyle, resulted in reduction of condylar height, which was a main feature of ICR. The mandibular retraction in ICR patients was caused by the rotating of the mandible rather than the receding of the condyle. References | Related Articles | Metrics

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Expression of miR-155 in Peripheral Blood and Tissues of Oral Lichen Planus Patients. Cao Zhi. 2016, 32(2): 185-188.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.020 Abstract ( 239 )   PDF (896KB) ( 203 )   Objective: To investigate the expression of miR-155 in peripheral blood and tissues of oral lichen planus patients. Methods: Eighty-five oral lichen planus patients were selected. They were divided into non-erosive type (n=48) and erosive type (n=37), according to the different conditions of the base of mucosa of lesions. Forty healthy subjects and forty-five normal tissues were selected as control group. The expressions of miR-155 in plasma and mononuclear cells in patients and the control group were detected by real-time quantitative PCR. And the expressions of miR-155 in mucosa of patients and normal mucosa were also detected. The efficacy of miR-155 in predicting the extent of lesions in patients was analyzed using ROC curve. Results: The relative expression levels of miR-155 in plasma and mononuclear cells of oral lichen planus patients (1.47±0.34) were significantly higher than the control group (0.95±0.12) (P<0.05). The relative expressions of miR-155 in plasma and tissue of erosive type group were higher than the non-erosive type group (P<0.05). ROC curve analysis showed that the relative expression of miR-155 in plasma of oral lichen planus patients in predicting the extent of the lesion was 0.807 (95%CI: 0.712-0.903). The sensitivity was 67.6% and specificity was 85.4%. The relative expression of miR-155 in tissues of oral lichen planus patients in predicting the extent of the lesion was 0.845 (95%CI: 0.761-0.928). The sensitivity was 70.3% and specificity was 87.5%. Conclusion: miR-155 might be used as a predictor index for the severity of the patient's lesions. References | Related Articles | Metrics

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Effect of Hemocoagulase Injection on Reducing Bleeding after Tooth Extraction. LI Ming-heng, YU Xin, SHAO Ben-chun, HE Ping, MA Wei-dong. 2016, 32(2): 189-190.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.021 Abstract ( 302 )   PDF (721KB) ( 273 )   Objective: To evaluate the haemostatic effect of hemocoagulase injection on the extraction of mandibular impacted wisdom teeth. Methods: Two hundred and sixty-seven cases were randomly divided into the experimental group (135 cases) and the control group (132 cases). The experimental group received the same extraction procedure as the control group, except a local injection of 1U hemocoagulase half an hour before the operation. Results: There was no bleeding case in the experimental group 40 minutes after the extraction, and 1 bleeding case after 24 hours. However, there was 5 bleeding cases 40 minutes after the extraction and 7 bleeding cases after 24 hours in the control group. Statistical analysis showed significant difference between two groups (P<0.05). Conclusion: Hemocoagulase injection before tooth extraction was effective on theh reducing of postoperative bleeding. References | Related Articles | Metrics

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Retrospective Analysis of the Prevalence of Impacted Teeth in Patients from the Central Hospital of Enshi Tujia and Miao Autonomous Prefecture. YANG Zai-bo, YANG Ran, ZHANG Jiang-e, ZHOU Yan-ling. 2016, 32(2): 191-193.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.022 Abstract ( 254 )   PDF (738KB) ( 219 )   Objecive: To study the prevalence of impacted teeth in patients from the Department of Stomatology of the Central Hospital of Enshi Tujia and Miao autonomous Prefecture. Methods: Data of 1121 patients were collected in the Central Hospital of Enshi from Jan.1, 2011 to Dec.30, 2013. With the help of CT, patients with impacted teeth were chosen and statistically analyzed according to different gender, nation and position. Results: The prevalence rate of the impacted teeth in orthodontic patients was 9.61%, and it was apparently higher than other areas of the country. Among these impacted teeth, the maxillary canines occupied the largest proportion (P<0.05), followed by supernumerary teeth, maxillary lateral incisor. Most cases presented only one impacted teeth (P<0.05). The prevalence of the impacted teeth showed no gender and ethnic differences. Conclusion: The prevalence of the impacted teeth was high in orthodontic patients, and the orthodontists should pay more attention in the clinical diagnosis. References | Related Articles | Metrics

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Clinical Analysis of the Treatment of Jaw Cyst Fenestration Decompression and Biomaterial Filling. LIU Min, XU Zheng-mao, HAN Qian-chao. 2016, 32(2): 194-196.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.023 Abstract ( 219 )   PDF (746KB) ( 233 )   Objective: To evaluate the decompression combined with calcium sulfate with clinical curative effect of the treatment of jaw cysts. Methods: From 2009 August -2013 year in August in our hospital were the large jaw cystic lesions in patients with a total of 70 cases, all patients were informed of the pros and cons of communication before operation, and the risk in patients with various kinds of treatment plans, according to the wishes of patients, divided into the observation group and the control group, the observation group of 35 cases received conservative treatment, I window decompression, the cysts reduced obviously after II small cyst curettage and calcium sulfate biological material filling operation, the control group of 35 cases, treated with traditional jaw cyst curettage, and calcium sulfate biological material filling, observed and compared two groups of patients with operation time, bleeding volume during operation, postoperative pain, postoperative, infections, cystic cavity size, degree of hyperplasia of bone thickness and bone density measurements, the incidence of recurrence and neuropathic complications were compared between the two groups at the same time. Results: The two groups of patients with operation time, intraoperative bleeding volume, postoperative pain and postoperative infection rate of phase difference was statistically significant (P<0.05); the two groups of patients after 6 months the index operation after 3 months were significantly improved, the observation group of patients after 3 months and after 6 month sac cavity was reduced in value, postoperative bone hyperplasia thickness value, postoperative bone density measurements were superior to the control group, the difference was statistically significant (P<0.05). All patients were followed up for 12 to 24 months, the control group 1 patients relapse, the observation group without recurrence, two groups of patients relapse rate difference does not have statistical significance (P<0.05). Conclusion: The clinical effect of large cystic lesions of the jaw is good with open window decompression, and it is worth to be popularized in clinic. References | Related Articles | Metrics

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Mandibular First Molar with Three Distal Canals Confirmed with CBCT: a Case Report. LU Guan-fan, SUN De-gang. 2016, 32(2): 197-198.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.024 Abstract ( 303 )   PDF (999KB) ( 323 )   This case report highlights the rare incidence of 3 canals in the distal root of a permanent mandibular first molar using CBCT References | Related Articles | Metrics

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Research Progress of Osseoperception in Field of Oral Implantology .ZHANG Yi-di, ZHOU Yan-min, ZHAO Jing-hui. 2016, 32(2): 199-201.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.026 Abstract ( 481 )   PDF (756KB) ( 528 )   Osseoperception is a new term based on osseointegration, which includes the mechanism about central nervous system and peripheral nerves. This article reviews the research progress of osseoperception in the field of oral implantology from two aspects. References | Related Articles | Metrics

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Applications of Common Molecular Detection Techniques in the Study of Microbial Diversity in Dental Caries among Children. ZHU Yu-juan, JIAO Kang-li, ZHOU Jian-ye, YU Zhan-hai, LI Zhi-qiang. 2016, 32(2): 202-204.  DOI: 10.13701/j.cnki.kqyxyj.2016.02.025 Abstract ( 247 )   PDF (806KB) ( 314 )   Dental caries is one of the most common chronic infectious diseases worldwide, and the incidence in children is increasing. Microorganisms are one of the main factors that cause dental caries. The species, mechanisms, and diversity of cariogenic microorganisms are hot topics in oral microbiology research. With the development of modern molecular biological techniques, molecular biological detection techniques are now widely used in microbiological research. These early detection and prevention methods play an important role in reducing the incidence of caries disease in children. This review focuses on the research advancement of some modern molecular biotechnology in oral microbial diversity research. In addition, it also made a prospect for the study of oral microbial diversity in children. References | Related Articles | Metrics