Effect of Silencing Long Noncoding RNA HOTAIR on Radiosensitivity of Tongue Squamous Cell Cells

doi:10.13701/j.cnki.kqyxyj.2023.04.006

Abstract

Abstract: Objective: To investigate the effect of down-regulated expression of long-chain non-coding RNA HOTAIR on the radiosensitivity of tongue squamous cell lines CAL-27 and SCC-9. Methods: Three HOTAIR interference sequences were designed and transfected into tongue squamous cell cells CAL-27 and SCC-9 by lentiviral vector. The interference efficiency was verified by real-time fluorescence quantitative PCR (qRT-PCR). The cells were divided into blank group (CAL-27, SCC-9), experimental group (si-HOTAIR CAL-27, si-HOTAIR SCC-9), and negative control group (si-NC CAL-27, si-NC SCC-9). The cells were irradiated with 8Gy electron line. MTT assay was used to detect the cell proliferation, scratch assay was used to detect the cell migration, and flow cytometry was used to detect the cell apoptosis. Results: Among three lectin vectors, si-2 and si-3 down-regulated HOTAIR expression in tongue squamous cell cells CAL-27 and SCC-9 (P<0.05), and si-3 had the highest interference efficiency (P<0.05). After receiving 8Gy electron line irradiation, compared with the control group (si-NC CAL-27 and si-NC SCC-9), the proliferative ability of the experimental group (si-HOTAIR CAL-27 and SI-NC SCC-9) was significantly decreased (P<0.05), apoptosis rate was increased [(23.87±1.97) % vs. (46.03±2.49) %, (15.99±0.76) % vs. (39.38±2.95) %)], and migration rate was decreased [65.85% vs. 58.82%, 47.81% vs. 37.78%]. The difference was statistically significant (P<0.05). Conclusion: Reducing the expression level of HOTAIR can increase the radiosensitivity of CAL-27 and SCC-9 of tongue squamous cell.

Key words: tongue squamous cell carcinoma, HOTAIR, migration, apoptosis, radiosensitivity

ZHANG Yang, GONG Zhongcheng, XIAO Xueling, LIU Pan, SU Weipeng, ZHAO Huarong. Effect of Silencing Long Noncoding RNA HOTAIR on Radiosensitivity of Tongue Squamous Cell Cells[J]. Journal of Oral Science Research, 2023, 39(4): 316-321.

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