Study on Platelet-Rich Fibrin Promoting Gingival Tissue Repair and Regeneration

doi:10.13701/j.cnki.kqyxyj.2022.02.011

Abstract

Abstract: Objective: To observe the effect of platelet-rich fibrin on the biological behavior of primary cells and tissue regeneration in the repair of gingival defects. Methods: PRF membrane was prepared. Scanning electron microscope and histopathology were used to observe the ultrastructure. CCK-8 and transwell test was used to detect cell proliferation and migration ability. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of soft tissue healing marker genes COL-I and TGF-β at different time points. HE staining and immunohistochemistry were used to observe tissue healing at different time points, and qRT-PCR was used to detect the expression of inflammatory factors IL-6, TNF-α, and TGF-β. Results: PRF could promote the proliferation, healing, and migration of human gingival fibroblast after trauma (P<0.05). The expressions of COL-I and TGF-β in the PRF group were higher than those in the DMEM group on day 7 (P< 0.05). The healing effect of PRF group was better than that of the control group. Histological observations showed that the PRF group had less inflammatory cell infiltration than the control group, and the PRF group had more angiogenesis than the control group. On day 7 after operation, the expression of IL-6 and TNF-α in the control group was higher than that in the PRF group, and the expression of TGF-β in the control group was lower than that of the PRF group (P<0.05). VEGF immunohistochemical results showed that the expression of positive cells in the PRF group was earlier than that in the control group. Conclusion: PRF can promote the expression of COL-I, TGF-β, and VEGF in the gums, reduce the local inflammation, and accelerate tissue healing.

Key words: platelet-rich fibrin, human gingival fibroblast, cell proliferation, cell movement and migration, wound healing

WANG Yuhui, LV Huixin, ZHANG Mingrui, WANG Yingying, REN Sicong, ZHENG Shikang, LIN Xinping, ZHOU Yanmin. Study on Platelet-Rich Fibrin Promoting Gingival Tissue Repair and Regeneration[J]. Journal of Oral Science Research, 2022, 38(2): 144-149.

References

[1] 韩子瑶,王翠,胡文杰,等.应用游离龈移植术增宽磨牙缺失区域角化组织利于种植治疗的临床观察(附1例3年随访报告)[J].中国实用口腔科杂志,2019,12(3):130-135.
[2] Bassetti RG, Stähli A, Bassetti MA, et al. Soft tissue augmentation around osseointegrated and uncovered dental implants: a systematic review [J]. Clin Oral Investig, 2017, 21(1):53-70.
[3] Torres P, Castro M, Reyes M, et al. Histatins, wound healing, and cell migration [J]. Oral Dis, 2018, 24(7):1150-1160.
[4] 汪汉池,周延民.富血小板纤维蛋白对软组织修复作用的研究进展[J].口腔医学,2019,39(2):171-174.
[5] Bi J, Intriago MFB, Koivisto L, et al. Leucocyte-and platelet-rich fibrin regulates expression of genes related to early wound healing in human gingival fibroblasts [J]. J Clin Periodontol, 2020, 47(7):851-862.
[6] 叶珊,方蛟,李雪,等.槲皮素对牙龈卟啉单胞菌脂多糖刺激的人牙龈成纤维细胞生物学行为的影响研究[J].中国实用口腔科杂志,2020,13(5):277-283.
[7] 卡米尔.富白细胞-血小板纤维蛋白对软组织愈合及炎症调节的研究[D].吉林大学,2019.
[8] 孙涛,和红兵.富血小板纤维蛋白在牙周组织再生中的研究进展[J].口腔医学研究,2020,36(9):808-810.
[9] Pitzurra L, Jansen IDC, de Vries TJ, et al. Effects of L-PRF and A-PRF+ on periodontal fibroblasts in in vitro wound healing experiments [J]. J Periodontal Res, 2020, 55(2):287-295.
[10] Yu P, Zhai Z, Lu H, et al. Platelet-rich fibrin improves fat graft survival possibly by promoting angiogenesis and adipogenesis, inhibiting apoptosis, and regulating collagen production [J]. Aesthet Surg J, 2020, 40(9):NP530-NP545.
[11] Lektemur Alpan A, Torumtay Cin G. PRF improves wound healing and postoperative discomfort after harvesting subepithelial connective tissue graft from palate: a randomized controlled trial [J]. Clin Oral Investig, 2020, 24(1):425-436.
[12] 唐一月.A-PRF/i-PRF对人牙龈成纤维细胞增殖分化作用的机制研究[D].兰州大学,2019.
[13] 唐一月,余占海.富血小板纤维蛋白对软组织修复与再生影响的研究进展[J].中华口腔医学研究杂志(电子版),2019,13(2):124-128.
[14] Petrescu BN, Mirica IC, Miron R, et al. Platelet rich fibrin as a gingival tissue regeneration enhancer [J]. J Dent Sci, 2021,16(1):536-539.
[15] 周菁,孙晓琳,齐曼霖,等.富血小板纤维蛋白在后牙即刻种植中的应用1例[J].口腔医学研究,2018,34(1):92-93.
[16] Zumarán CC, Parra MV, Olate SA, et al. The 3 R's for platelet-rich fibrin: A "super" tri-dimensional biomaterial for contemporary naturally-guided oro-maxillo-facial soft and hard tissue repair, reconstruction and regeneration [J]. Materials (Basel), 2018 ,11(8):1293.