Effect of Chloroquine on the Proliferation of Rats Bone Marrow Mesenchymal Stem Cells Irritated by MT01.

doi:DOI

Abstract

Abstract: Objective: To confirm that MT01 may influence the biological properties of bone marrow mesenchymal stem cells (BMSCs) via endosome by detecting the effect of chloroquine (CQ) on the promotion of Wistar rat BMSCs proliferation by MT01. Methods: The third generation of Wistar rat BMSCs were cultured with different working concentrations of CQ for 24,48,72 and 96 hours, in order to detect the effect of CQ on BMSCs proliferation in dose-response relationship and aging relationship. Then, MTT assay was employed to detect the influence of CQ on MT01-stimulated BMSCs proliferation at different concentrations and different time points by adding the 1mg/L working concentration of MT01. Results: Appropriate concentration of CQ (4mg//L) could promote BMSCs proliferation. CQ exhibited the strongest promotion to BMSCs proliferation after 48 hours. Low concentrations of CQ (1mg/L and 2mg/L) inhibited the MT01-mediated promotion of BMSCs proliferation. Conclusion: CQ has an inhibitory effect on the MT01-mediated promotion of BMSCs proliferation, suggesting that MT01 may play a role by entering the endosome in BMSCs.

CLC Number:

R780.1

LI Wei-ting, LI Bo, DING Zi-qing, CUI Ye, GAO Han, LIU Yin, SHEN Yu-qin.. Effect of Chloroquine on the Proliferation of Rats Bone Marrow Mesenchymal Stem Cells Irritated by MT01.[J]. Journal of Oral Science Research, 2015, 31(11): 1061-1063.

References

[1] Yang G, Wan M, Zhang Y, et al. Inhibition of a Crich oligodeoxynucleotide on activation of immune cells in vitro and enhancement of antibody response in mice [J]. Immunology, 2010, 131(4)∶501-512
[2] Yasuda K, Rutz M, Schlatter B, et al. CpG motifindependent activation of TLR9 upon endosomal translocation of "natural" phosphodiester DNA [J]. Eur J Immunol, 2006, 36(2)∶431-436
[3] Shen Y, Feng Z, Lin C, et al. An oligodeoxynucleotide that induces differentiation of bone marrow mesenchymal stem cells to osteoblasts in vitro and reduces alveolar bone loss in rats with periodontitis [J]. Int J Mol Sci, 2012, 13(3)∶2877-2892
[4] 申玉芹,孙新华,于丽,等.不同 CpG ODN对大鼠骨髓间充质干细胞增殖作用的研究[J].口腔医学研究,2009,25(6)∶723-725
[5] Nagar J, Ranade S, Kamath V, et al. Therapeutic potential of chloroquine in a murine model of inflammatory bowel disease [J]. Int Immunopharmacol, 2014, 21(2)∶328-335
[6] Bijker EM, Nganou-Makamdop K, van Gemert GJ, et al. Studying the effect of chloroquine on sporozoite-induced protection and immune responses in Plasmodium berghei malaria [J]. Malar J, 2015 Mar 26;14:130. doi: 10.1186/s12936-015-0626-2
[7] Joshi P, Chakraborti S, Ramirez-Vick JE, et al. The anticancer activity of chloroquine-gold nanoparticles against MCF-7 breast cancer cells [J]. Colloids Surf B Biointerfaces, 2012, 95∶195-200
[8] Tuomela J, Sandholm J, Kauppila JH, et al. Chloroquine has tumor inhibitory and tumor promoting effects in triple negative breast cancer [J]. Oncol Lett, 2013, 6(6)∶1665-1672
[9] Juge N, Moriyama S, Miyaji T, et al. Plasmodium falciparum chloroquine resistance transporter is a H+-coupled polyspecific nutrient and drug exporter [J]. Proc Natl Acad Sci U S A, 2015, 112(11)∶3356-3361
[10] Zhou D, Liu Y, Xu L H, et al. Chloroquine Differentially Modulates Inflammatory Cytokine Expression in RAW 264.7 Cells in Response to Inactivated Staphylococcus aureus [J]. Inflammation, 2015, 38(2)∶745-755
[11] Rungsihirunrat K, Muhamad P, Chaijaroenkul W, et al. Plasmodium vivax Drug Resistance Genes; Pvmdr1 and Pvcrt-o Polymorphisms in Relation to Chloroquine Sensitivity from a Malaria Endemic Area of Thailand [J]. Korean J Parasitol, 2015, 53(1)∶43-49
[12] van Kasteren SI, Overkleeft HS. Endo-lysosomal proteases in antigen presentation [J]. Curr Opin Chem Biol, 2014, 23∶8-15
[13] Chopra A, Saluja M, Venugopalan A. Effectiveness of chloroquine and inflammatory cytokine response in patients with early persistent musculoskeletal pain and arthritis following chikungunya virus infection [J]. Arthritis Rheumatol, 2014, 66(2)∶319-326
[14] Lee BL, Barton GM. Trafficking of endosomal Toll-like receptors [J]. Trends Cell Biol, 2014, 24(6)∶360-369
[15] Hazeki K, Nigorikawa K, Hazeki O. The Role of PIKfyve in Toll-Like Receptor-Mediated Responses[M]//Inflammation and Immunity in Cancer. Springer Japan, 2015∶49-62