Regulatory Effects of XLαs on Cranial Bone Development through Influencing Mitochondrial Function

doi:10.13701/j.cnki.kqyxyj.2024.07.004

Abstract

Abstract: Objective: To investigate the role and underlying molecular mechanism of extra-large stimulatory G protein alpha subunit (XLαs) in cranial bone development. Methods: Utilizing a genetically engineered mouse model lacking the specific exon 1 of XLαs, cranial morphology were analyzed through alician blue and alizarin red staining. Primary cranial osteoblasts were isolated and cultured in vitro, and induced for osteogenic differentiation. Gene expression patterns and functional analysis were assessed through quantitative RT-PCR and RNA-seq. Results: Knockout of XLαs in mice led to notably increased cranial suture width (P<0.05), expanded presence of unmineralized areas within cranial bones (P<0.001), and a significant reduction in osteogenic differentiation (P<0.01). Further, RNA-seq analysis revealed that XLαs deficiency disrupted mitochondrial function. Conclusion: XLαs plays a critical regulatory role in the cranial bone development by modulating mitochondrial function, which provides a novel therapeutic strategy for treating cranial developmental anomalies associated with GNAS mutations.

Key words: extra-large stimulatory G protein alpha subunit, cranial development, mineralization, mitochondria

ZUO Yiyi, HE Qing. Regulatory Effects of XLαs on Cranial Bone Development through Influencing Mitochondrial Function[J]. Journal of Oral Science Research, 2024, 40(7): 587-592.

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