Effectes of Fibronectin-immobilized Microgroove Titanium Plate on Human Gingival Fibroblasts.

Abstract

Abstract: Objective: To study the effects of fibronectin (FN)-immobilized microgroove titanium plate on human gingival fibroblasts (HGF). Methods: FN was immobilized to the microgroove of titanium plate after treated with Tresyl chloride. XPS was used to analyze the surface elements. CCK8 was used to evaluate the cell proliferation of HGFs at 6h, 12h, 1d, 3d, 5d and 7d after treated. The ELISA and real-time PCR were used to detect the secretion and expression of collagen I in HGFs at 5d. Results: All the cell number in the experimental group was higher than control group at the predetermined time (P<0.05). The stretch rate of HGFs in the experimental group was higher than control group at 3d (P<0.05). The secretion and mRNA level of collagen I in the experimental group were higher than those of control group at 5d (P<0.05). Conclusion: The FN-immobilized microgroove titanium plate could promote the proliferation, adhesion and secretion function of HGFs.

CLC Number:

R783.2

HUANG Si-jia, XU Zhi-qiang, FAN Jian, GUO Jian-bin, ZHOU Lin, CHEN Jiang.. Effectes of Fibronectin-immobilized Microgroove Titanium Plate on Human Gingival Fibroblasts.[J]. Journal of Oral Science Research, 2015, 31(11): 1113-1117.

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