Abstract: Objective: To investigate the capability of tooth tissue-bone complex-like structure in vivo formed by rDECs+rDMCs-nHAC/PLA compounds induced by different growth factors. 〖WT5”HZ〗Methods: Rat dental epithelial cells (rDECs)and rat dental mesenchymal cells (rDMCs) were isolated, cultured and identified.The cell-scaffold compounds were transplanted into athymic mice subcutaneously and taken out after 3 or 5 months. The capability of tooth tissue-bone complex-like structure formed by the graftswas detected. Results: BothrDECs and rDMCscould be isolated, cultured and identified.After 3 months, the osteoid, new bone and blood vessel were found in group 1 to 3. The osteogenesis in group2 (TGF-β1+BMP4)was most powerful. There were only a few collagen fibers and blood vessels in the control (group 4). After 5 months, tooth root-like structure without pulp cavity was seen in group 1 and 3. In group 2, new bone and tooth-type tissueswere formed. Ameloblastin and CAP were expressed positively in emailloid and cementoid tissues respectively. In group 4, there was new bone but no tooth-like structure. Conclusion: The most powerful way that affects theosteogenesis and tooth-forming forrDECs+rDMCs-nHAC/PLA grafts is TGF-β1+BMP4.

Key words: rDECs , rDMCs , nHAC/PLA