[doi] 10.13701/j.cnki.kqyxyj.2016.07.016Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.

doi:10.13701/j.cnki.kqyxyj.2016.07.016

Abstract

Abstract: Objective: To observe the expression of IL-27 on different cells in the periapical tissues of human chronic periapical diseases. Methods: The periapical tissue specimens of 60 donors including healthy control (n=20), periapical granuloma (n=20) and radicular cyst (n=20) were stained with hematoxylin and eosin for histopathology, and double-immunofluorescence for identification of IL-27-tryptase (MCs), IL-27-CD14 (MPs) and IL-27-CD31 (ECs) double-positive cells. Results: Compared with the healthy control, the percentages (%) of double-positive cells were significantly increased in human chronic periapical diseases (P<0.01); the percentage of IL-27-tryptase double positive cells in radicular cyst was significantly higher than those in periapical granuloma (P<0.01); the percentages of IL-27-CD14 and IL-27-CD31 double-positive cells in periapical granuloma were not significant different from those in radicular cyst. Conclusion: IL-27 expressed in MCs, MPs and ECs of human chronic periapical diseases with different degree; IL-27-tryptase double-positive cells may participate in the pathogenic mechanism of chronic periapical diseases, especially in the formation of fibrous tissue in periapical cysts.

Key words: Periapical diseases , Interleukin-27 , Mast cells , Mononuclear phagocyte cells , Endothelial cells

CLC Number:

R781.4

LI Juan, SHEN Su-qian, LIANG Ze-zi, LIN Qi-lei, HUANG Shi-guang.. [doi] 10.13701/j.cnki.kqyxyj.2016.07.016

Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.[J]. Journal of Oral Science Research, 2016, 32(7): 728-732.

References

[1] Sonobe Y, Yawata I, Kawanokuchi J, et al. Production of IL-27 and other IL-12 family cytokines by microglia and their subpopulations [J]. Brain Res, 2005, 1040(1-2)∶202-207
[2] Fitzgerald DC, Ciric B, Touil T, et al. Suppressive effect of IL-27 on encephalitogenic Th17 cells and the effector phase of experimental autoimmune encephalomyelitis [J]. J Immunol, 2007, 179(5)∶3268-3275
[3] Jung JY, Roberts LL, Robinson CM. The presence of interleukin-27 during monocyte-derived dendritic cell differentiation promotes improved antigen processing and stimulation of T cells [J]. Immunology, 2015, 144(4)∶649-660
[4] Teixeira-Salum TB, Rodrigues DB, Gervasio AM, et al. Distinct Th1, Th2 and Treg cytokines balance in chronic periapical granulomas and radicular cysts [J]. J Oral Pathol Med, 2010, 39(3)∶250-256
[5] Costa NL, Oton-Leite AF, Cheim AP, et al. Density and migration of mast cells in lip squamous cell carcinoma and actinic cheilitis [J]. Histol Histopathol, 2009, 24(4)∶457-465
[6] Batista A, Rodini C, Lara V. Quantification of mast cells in different stages of human periodontal disease [J]. Oral Dis, 2005, 11(4)∶249-254
[7] Kastelein RA, Hunter CA, Cua DJ. Discovery and biology of IL-23 and IL-27: Related but functionally distinct regulators of inflammation [J]. Annu Rev Immunol,2007, 25(2)∶221-242
[8] Stumhofer JS, Hunter CA. Advances in understanding the anti-inflammatory properties of IL-27 [J]. Immunol Lett, 2008, 117(2)∶123-130
[9] Stashenko P, Teles R, D'Souza R. Periapical inflammatory responses and their modulation [J]. Crit Rev Oral Biol Med, 1998, 9(4)∶498-521
[10] Kawashima N, Okiji T, Kosaka T, et al. Kinetics of macrophages and lymphoid cells during the development of experimentally induced periapical lesions in rat molars: a quantitative immunohistochemical study [J]. J Endod, 1996, 22(6)∶311-316
[11] Metzger Z. Macrophages in periapical lesions [J]. Endod Dent Traumatol, 2000, 16(1)∶1-8
[12] Nagy JA, Benjamin L, Zeng H, et al. Vascular permeability, vascular hyperpermeability and angiogenesis [J]. Angiogenesis, 2008, 11(2)∶109-119
[13] Drazic R, Sopta J, Minic AJ. Mast cells in periapical lesions: potential role in their pathogenesis [J]. J Oral Pathol Med, 2010, 39(3)∶257-262
[14] Rodini CdO, Batista AC, Lara VS. Comparative immunohistochemical study of the presence of mast cells in apical granulomas and periapical cysts: possible role of mast cells in the course of human periapical lesions [J]. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 2004, 97(1)∶59-63
[15] 张海军,刘红,白黎露.肥大细胞类胰蛋白酶(MCT)在口腔鳞癌患者血清中表达水平的研究[J].口腔医学研究,2014,30(12)∶1166-1168
[16] Fonseca-Silva T, Santos CC, Alves LR, et al. Detection and quantification of mast cell, vascular endothelial growth factor, and microvessel density in human inflammatory periapical cysts and granulomas [J]. Int Endod J, 2012, 45 (9)∶859-864

[doi] 10.13701/j.cnki.kqyxyj.2016.07.016

Immunomodulatory Activity of Interleukin-27 in Human Chronic Periapical Diseases.

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