Abstract: Objective: To observe the angiogenic potential of coculture of dental pulp stem cells (hDPSCs) induced by dentin extract (DE) and human umbilical vein endothelial cells (HUVECs) during dental pulp regeneration. Methods: hDPSCs were induced by DE for 7 and 14 days. Real-time PCR and Western-blot assay were used to detect the expression of DSPP, DMP-1 and DSP in hDPSCs. Angiogenic assays were divided into five groups: HUVECs, induced hDPSCs and HUVECs cocultured with induced hDPSCs in the ratios of 5:1, 5:5 and 5:10. The tubular length and branching point number were detected after 3,6 and 9 h after the cells were seeded on the matrigel. Results: The expressions of DSPP, DMP-1 and DSP in induced group were up-regulated. Angiogenic assay showed that vessel-like structures formed more than other groups when HUVECs: induced hDPSCs cocultured ratio was at 5:1 at 3h (P＜0.05). While vessel-like structures in 5:5 and 5:10 coculture groups were less than those in HUVECs group at all the time points (P＜0.05). Conclusion: Coculture of hDPSCs induced by DE and HUVECs in a certain proportion can enhance the angiogenic potential of dental pulp tissues.