TNF-α Increasing the Angiogenic Potential of Dental Pulp Cells and Endothelial Cells in Co-culture System.

doi:10.13701/j.cnki.kqyxyj.2019.10.014

Abstract

Abstract: Objective: To investigate the effects of human dental pulp stem cells (hDPSCs) and human umbilical vein endothelial cells (HUVECs) in the co-culture system on formation of endothelial tubules and vascular networks. Methods: hDPSCs were co-cultured with HUVECs at a ratio of 1∶5. Matrigel assay were performed on the co-culture system to detect the total branching lengths and the number of branches. CCK-8 assay was used to examine the effect of TNF-α on the cell proliferation. LIVE/DEAD viability/cytotoxicity kit was used to detect the mammalian cells. Results: Matrigel assay showed the total branching length. The number of branches formed in the experimental groups treated with 50 μg/L TNF-α was significantly higher than that of the control group. The decomposition time of the tubule structures was significantly prolonged (P<0.05). After treated with 50 μg/L TNF-α, there was no significant changes in the proliferation of co-cultured cells (P>0.05) . Conclusion: TNF-α enhanced the angiogenic ability of hDPSCs and HUVECs in the co-culture system in vitro.

Key words: dental pulp stem cell, human umbilical vein endothelial cells, dental pulp regeneration, angiogenesis

AN Li, SHEN Shuai, WANG Luyao, LI Yong, NIU Yumei, PAN Shuang. TNF-α Increasing the Angiogenic Potential of Dental Pulp Cells and Endothelial Cells in Co-culture System.[J]. Journal of Oral Science Research, 2019, 35(10): 966-969.

References

[1] Carmeliet P, Jain RK. Molecular mechanisms and clinical applications of angiogenesis [J]. Nature, 2011, 473(7347):298-307.
[2] Bhadada SV, Goyal BR, Patel MM. Angiogenic targets for potential disorders [J]. Fundam Clin Pharmacol, 2011, 25(1):29-47.
[3] Carmeliet P, Jain RK. Principles and mechanisms of vessel normalization for cancer and other angiogenic diseases [J]. Nat Rev Drug Discov, 2011, 10(6):417-427.
[4] Folkman J. Is angiogenesis an organizing principle in biology and medicine ?[J]. J Pediatr Surg, 2007, 42(1):1-11.
[5] Moioli EK, Clark PA, Chen M, et al. Synergistic actions of hematopoietic and mesenchymal stem/progenitor cells in vascularizing bioengineered tissues [J]. PLoS One, 2008, 3(12):e3922.
[6] Tran-Hung L, Mathieu S, About I .Role of human pulp fibroblasts in angiogenesis [J]. J Dent Res, 2006, 85(9):819-823.
[7] Grando Mattuella L, Poli de Figueiredo JA, Nör JE, et al. Vascular endothelial growth factor receptor-2 expression in the pulp of human primary and young permanent teeth [J]. J Endod, 2007, 33(12):1408-1412.
[8] 于莉,李淑慧,袁萍,等.TNF-α对根尖乳头干细胞体外增殖及成牙成骨向分化能力的影响[J].口腔医学研究,2016,32(4):343-346.
[9] Laschke MW, Harder Y, Amon M, et al. Angiogenesis in tissue engineering: breathing life into constructed tissue substitutes [J]. Tissue Eng, 2006, 12(8):2093-2104.
[10] He L, Pan S, Li Y, et al. Increased proliferation and adhesion properties of human dental pulp XE dental pulp stem cells in PLGA scaffolds via simulated microgravity [J]. Int Endod J, 2016, 49(2):161-173.
[11] Rahaman MN, Mao JJ. Stem cell-based composite tissue constructs for regenerative medicine [J]. Biotechnol Bioeng, 2005, 91(3):261-284.
[12] Shi L, Fu S, Fahim S, et al. TNF-αlpha stimulation increases dental pulp stem cell migration in vitro through integrin alpha-6 subunit upregulation [J]. Arch Oral Biol, 2017, 75:48-54.
[13] Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs)in vitro and in vivo [J]. Proc Natl Acad Sci U S A, 2000, 97(25):13625-13630.
[14] Nakashima M, Akamine A. The application of tissue engineering to regeneration of pulp and dentin in endodontics [J]. J Endod, 2005, 31(10):711-718.
[15] Sieveking DP, Ng MK. Cell therapies for therapeutic angiogenesis: back to the bench [J]. Vasc Med, 2009, 14(2):153-166.
[16] Yoshida S, Ono M, Shono T, et al. Involvement of interleukin-8, vascular endothelial growth factor, and basic fibroblast growth factor in tumor necrosis factor alpha-dependent angiogenesis [J]. Mol Cell Biol, 1997, 17(7):4015-23.
[17] Howard C, Murray PE, Namerow KN. Dental pulp stem cell migration [J]. J Endod, 2010, 36(12):1963-1966.