成牙骨质细胞矿化过程中可变剪接事件分析

doi:10.13701/j.cnki.kqyxyj.2021.07.012

口腔医学研究 ›› 2021, Vol. 37 ›› Issue (7): 632-636.DOI: 10.13701/j.cnki.kqyxyj.2021.07.012

成牙骨质细胞矿化过程中可变剪接事件分析

黄汉韬¹, 王晓璇^1,2, 江晨曦¹, 马莉¹, 姚斯琦¹, 刘欢^1,2, 曹正国^1,2*

1.武汉大学口腔医学院口腔基础医学省部共建国家重点实验室培训基地和口腔生物医学教育部重点实验室湖北武汉 430079;
2.武汉大学口腔医院牙周科湖北武汉 430079

收稿日期:2021-01-07 出版日期:2021-07-28 发布日期:2021-07-13
通讯作者: * 曹正国,E-mail:caozhengguo@whu.edu.cn
作者简介:黄汉韬(1993~ ),男,安徽人,硕士在读,研究方向:牙周病学。
基金资助:
国家自然科学基金(编号:81870776)

Bioinformatics Analysis of Alternative Splicing Events during Mouse Cementoblast Mineralization

HUANG Hantao¹, WANG Xiaoxuan^1,2, JIANG Chenxi¹, MA Li¹, YAO Siqi¹, LIU Huan^1,2, CAO Zhengguo^1,2*

1. The State Key Laboratory Breeding Base of Basic Science of Stomatology and Key Laboratory for Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China;
2. Department of Periodontology, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China

Received:2021-01-07 Online:2021-07-28 Published:2021-07-13

摘要/Abstract

摘要： 目的:生物信息学分析成牙骨质细胞矿化过程中可变剪接事件以及差异可变剪接基因富集到的生物学功能和信号通路。方法:小鼠成牙骨质细胞OCCM30矿化诱导0、7和14 d。实时荧光定量聚合酶链反应(qRT-PCR)检测骨钙素(bone gamma-carboxyglutamate protein,Bglap)和成骨细胞特异性转录因子(Osterix,Osx)的相对表达量来验证矿化结果。生物信息学分析可变剪接事件类型。Gene Ontology (GO)和Kyoto Encyclopedia of Genes and Genomes (KEGG)分别分析差异可变剪接基因可能富集到的生物学功能和信号通路。结果:Bglap和Osx在成牙骨质细胞矿化过程中表达量升高。与0 d相比,在矿化第7天和第14天时分别有1702个和1682个可变剪接事件发生。差异可变剪接基因主要与蛋白质丝氨酸/苏氨酸激酶活性、肽基赖氨酸修饰、Wnt信号通路和血管内皮生长因子信号通路等有关。结论:成功矿化诱导成牙骨质细胞。通过生信分析确定可变剪接事件以及差异可变剪接基因可能在成牙骨质细胞矿化过程中发挥作用,有望为今后牙骨质再生和牙周再生提供新的研究思路。

关键词: 成牙骨质细胞, 可变剪接, 矿化

Abstract: Objective: To bioinformational analyze the alternative splicing events and biofunctions and significant signaling pathways of differential alternative splicing genes during mouse cementoblasts (OCCM30) mineralization. Methods: OCCM30 were cultured in mineralized medium for 0, 7, and 14 days. The relative gene expression levels of bone gamma-carboxyglutamate protein (Bglap) and Osterix (Osx) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were performed to enrich potential biofunctions and significant signaling pathways. Results: Compared with those on day 0, 1702 and 1682 alternative splicing events were detected on day 7 and 14, respectively. Differential alternative splicing genes were associated with protein serine/threonine kinase activity, peptidyl-lysine modification, Wnt signaling pathway, and VEGF (vascular endothelial growth factor) signaling pathway and so on. Conclusion: Cementoblasts were successfully induced by mineralized medium. Using bioinformatics analysis, alternative splicing events were identified and differential alternative splicing genes may exert functions on cementoblast mineralization, which may provide a novel insight for cementogenesis and periodontal regeneration in the future.

Key words: cementoblast, alternative splicing, mineralization

黄汉韬, 王晓璇, 江晨曦, 马莉, 姚斯琦, 刘欢, 曹正国. 成牙骨质细胞矿化过程中可变剪接事件分析[J]. 口腔医学研究, 2021, 37(7): 632-636.

HUANG Hantao, WANG Xiaoxuan, JIANG Chenxi, MA Li, YAO Siqi, LIU Huan, CAO Zhengguo. Bioinformatics Analysis of Alternative Splicing Events during Mouse Cementoblast Mineralization[J]. Journal of Oral Science Research, 2021, 37(7): 632-636.

参考文献

[1] Hajishengallis G, Chavakis T, Lambris JD. Current understanding of periodontal disease pathogenesis and targets for host-modulation therapy [J]. Periodontol 2000, 2020, 84(1):14-34.
[2] Loos BG, Van Dyke TE. The role of inflammation and genetics in periodontal disease [J]. Periodontol 2000, 2020, 83(1):26-39.
[3] Bosshardt DD, Selvig KA. Dental cementum: the dynamic tissue covering of the root [J]. Periodontol 2000, 1997, 13:41-75.
[4] Bosshardt DD. Are cementoblasts a subpopulation of osteoblasts or a unique phenotype? [J]. J Dent Res, 2016, 84(5):390-406.
[5] Vaquette C, Pilipchuk SP, Bartold PM, et al. Tissue engineered constructs for periodontal regeneration: current status and future perspectives [J]. Adv Healthc Mater, 2018, 7(21):e1800457.
[6] Cao Z, Liu R, Zhang H, et al. Osterix controls cementoblast differentiation through downregulation of Wnt-signaling via enhancing DKK1 expression [J]. Int J Biol Sci, 2015, 11(3):335-344.
[7] Wang C, Liao H, Sun H, et al. MicroRNA-3064-3p regulates the differentiation of cementoblasts through targeting DKK1 [J]. J Periodontal Res, 2018, 53(5):705-713.
[8] 王川,廖海清,曹正国.微阵列芯片分析成牙骨质细胞矿化过程中microRNA表达谱的差异[J].口腔医学研究,2017,33(2):135-139.
[9] Liao HQ, Liu H, Sun HL, et al. MiR-361-3p/Nfat5 signaling axis controls cementoblast differentiation [J]. J Dent Res, 2019, 98(10):1131-1139.
[10] Ule J, Blencowe BJ. Alternative splicing regulatory networks: functions, mechanisms, and evolution [J]. Mol Cell, 2019, 76(2):329-345.
[11] Planells B, Gomez-Redondo I, Pericuesta E, et al. Differential isoform expression and alternative splicing in sex determination in mice [J]. BMC Genomics, 2019, 20(1):202.
[12] Rossner P, Vrbova K, Strapacova S, et al. Inhalation of ZnO nanoparticles: splice junction expression and alternative splicing in mice [J]. Toxicol Sci, 2019, 168(1):190-200.
[13] Li J, Wang C, Feng G, et al. Rbm14 maintains the integrity of genomic DNA during early mouse embryogenesis via mediating alternative splicing [J]. Cell Prolif, 2020, 53(1):e12724.
[14] Park JW, Fu S, Huang B, et al. Alternative splicing in mesenchymal stem cell differentiation [J]. Stem Cells, 2020, 38(10):1229-1240.
[15] Jin Y, Dong H, Shi Y, et al. Mutually exclusive alternative splicing of pre-mRNAs [J]. Wiley Interdiscip Rev RNA, 2018, 9(3):e1468.
[16] Hwang J, Yokota T. Recent advancements in exon-skipping therapies using antisense oligonucleotides and genome editing for the treatment of various muscular dystrophies [J]. Expert Rev Mol Med, 2019, 21:e5.
[17] Leopold AV, Chernov KG, Verkhusha VV. Optogenetically controlled protein kinases for regulation of cellular signaling [J]. Chem Soc Rev, 2018, 47(7):2454-2484.
[18] Zhao R, Tao L, Qiu S, et al. Melatonin rescues glucocorticoid-induced inhibition of osteoblast differentiation in MC3T3-E1 cells via the PI3K/AKT and BMP/Smad signalling pathways [J]. Life Sci, 2020, 257:118044.
[19] Maeda K, Kobayashi Y, Koide M, et al. The regulation of bone metabolism and disorders by Wnt signaling [J]. Int J Mol Sci, 2019, 20(22):5525.
[20] 关孟莹,何丽娜,潘爽,等.经典Wnt信号通路对hDPSCs增殖、迁移和成牙本质向分化的影响[J].口腔医学研究,2020,36(9):861-865.
[21] Tang Z, Xie H, Jiang S, et al. Safflower yellow promotes angiogenesis through p-VHL/ HIF-1alpha/VEGF signaling pathway in the process of osteogenic differentiation [J]. Biomed Pharmacother, 2018, 107:1736-1743.
[22] Hamushan M, Cai W, Zhang Y, et al. High-purity magnesium pin enhances bone consolidation in distraction osteogenesis model through activation of the VHL/HIF-1alpha/VEGF signaling [J]. J Biomater Appl, 2020, 35(2):224-236.

成牙骨质细胞矿化过程中可变剪接事件分析

Bioinformatics Analysis of Alternative Splicing Events during Mouse Cementoblast Mineralization

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	吴文治, 谢志坚. 固定矫治后牙面白垩斑的治疗研究进展[J]. 口腔医学研究, 2021, 37(6): 489-492.
[2]	田青鹭, 王艳. 生物陶瓷材料在根尖屏障术中的应用进展[J]. 口腔医学研究, 2021, 37(3): 191-194.
[3]	俞少玲, 臧睿觉, 李姮. 卵黄高磷蛋白调控生物玻璃对牙本质的再矿化[J]. 口腔医学研究, 2021, 37(2): 122-125.
[4]	田子璐, 朱松. 诱导牙本质再矿化以堵塞牙本质小管的研究进展[J]. 口腔医学研究, 2021, 37(1): 11-14.
[5]	王岩松, 刘建林, 陈晓霞 ,白姝婷. Exendin4-PEGDA/HA矿化水凝胶的制备及体外成骨作用的研究[J]. 口腔医学研究, 2020, 36(8): 731-735.
[6]	岳阳丽, 马贵霞, 刘翠丽, 贺贵天. 生物活性玻璃促进牙本质再矿化的时间相关性体外研究[J]. 口腔医学研究, 2020, 36(12): 1137-1141.
[7]	王金凤, 车静怡, 刘会梅, 朱兴国, 孙文章, 潘爽. 黄瓜籽醇提取物对早期釉质龋再矿化的影响[J]. 口腔医学研究, 2020, 36(1): 65-69.
[8]	胡蝶,张凌琳. 口腔来源蛋白及多肽诱导牙釉质仿生矿化的研究进展[J]. 口腔医学研究, 2019, 35(6): 517-520.
[9]	黄珺, 郭继华, 樊明文. SRSF3调控CTLA-4外显子3的可变剪接的研究[J]. 口腔医学研究, 2019, 35(1): 94-97.
[10]	宋金花, 刘宗仁, 刘文超, 张旭, 李彦秋. 羧甲基壳聚糖-溶菌酶纳米凝胶稳定无定形磷酸钙促进脱矿牙釉质再矿化[J]. 口腔医学研究, 2018, 34(8): 828-831.
[11]	王欣,张程,李天成,陶贵渝,段沛沛,邹淑娟. 一种新型牙骨质细胞系的矿化特性研究[J]. 口腔医学研究, 2018, 34(12): 1324-1328.
[12]	王川, 廖海清, 曹正国. 微阵列芯片分析成牙骨质细胞矿化过程中microRNA表达谱的差异[J]. 口腔医学研究, 2017, 33(2): 135-139.
[13]	杨薇, 王健平, 刘鑫喆, 黄歆博, 李雪. 氟纳米羟基磷灰石对早期根面龋再矿化效果的实验研究[J]. 口腔医学研究, 2017, 33(2): 191-193.
[14]	梁向阳,孔晶晶,李春年,李安. 不同材料在碳酸饮料所致脱矿釉质再矿化中的作用[J]. 口腔医学研究, 2017, 33(12): 1274-1277.
[15]	邱雨 ,任嫒姝 ,庞琴 ,马小娟 ,付钢. 信号素3A对成牙骨质细胞系OCCM-30增殖和矿化功能影响的实验研究[J]. 口腔医学研究, 2016, 32(5): 445-448.