Jumonji结构域蛋白3调节人牙周膜细胞的炎症反应

doi:10.13701/j.cnki.kqyxyj.2022.05.008

口腔医学研究 ›› 2022, Vol. 38 ›› Issue (5): 424-428.DOI: 10.13701/j.cnki.kqyxyj.2022.05.008

Jumonji结构域蛋白3调节人牙周膜细胞的炎症反应

王璞玉¹, 索来^2*

1.天津市口腔医院牙体牙髓二科天津 300041;
2.天津市口腔医院国际诊疗中心天津 300041

收稿日期:2021-11-22 出版日期:2022-05-28 发布日期:2022-05-20
通讯作者: *索来,E-mail:625493282@qq.com
作者简介:王璞玉(1991~ ),女,河北人,博士,主治医师,主要从事牙体牙髓病学临床基础相关研究。
基金资助:
天津市口腔医院科技基金(青年项目)(编号:2019BSQN01、2019BSQN02) 天津市卫健委青年项目(编号:TJWJ2021QN066) 天津市医学重点学科(专科)建设项目

Jumonji Domain-containing Protein 3 Regulates the Inflammatory Response of Human Periodontal Ligament Cells

WANG Puyu¹, SUO Lai^2*

1. Department Ⅱ of Endodontics, Tianjin Stomatological Hospital, Tianjin 300041, China;
2. Department of International VIP Dental Clinic, Tianjin Stomatological Hospital, Tianjin 300041, China

Received:2021-11-22 Online:2022-05-28 Published:2022-05-20

摘要/Abstract

摘要： 目的: 研究三甲基化组蛋白H3赖氨酸K27(histone 3 lysine 27 trimethylation, H3K27me3)的去甲基化酶Jumonji结构域蛋白3(Jumonji domain-containing protein 3, Jmjd3)在人牙周膜细胞炎症反应中的调节作用。方法: 分别使用牙龈卟啉单胞菌(Porphyromonas gingivalis,p.g)的脂多糖(lipopolysaccharide,LPS)和肿瘤坏死因子肿瘤坏死因子-α (tumor necrosis factor-α,TNF-α) 刺激人牙周膜细胞,建立炎症模型。通过实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction, Q-PCR)和免疫荧光检测Jmjd3的表达情况。后通过沉默Jmjd3,在Q-PCR和Western blot确定沉默效果后对其可能的下游靶基因进行初步筛选。结果: Jmjd3在炎症的人牙周膜细胞中表达会上调。沉默Jmjd3后,炎症介导的白细胞介素-6和白细胞介素-12b的上调受到抑制。结论: Jmjd3参与人牙周膜细胞炎症反应,并能够调节白细胞介素-6、白细胞介素-12b的表达。

关键词: Jumonji结构域蛋白3, 牙周膜细胞, 炎症反应

Abstract: Objective: To investigate the role of the histone 3 lysine 27 trimethylation (H3K27me3) demethylase Jumonji domain-containing protein 3 (Jmjd3) in the regulation of the inflammatory response of human periodontal ligament cells (HPDLs). Methods: HPDLs were stimulated with lipopolysaccharide (LPS) from p.g or tumor necrosis factor-α. The expression of Jmjd3 in HPDLs was examined by quantitative real-time polymerase chain reaction (Q-PCR) and immunofluorescence. After confirmed the depletion of Jmjd3 by Q-PCR and Western blot, potential target genes were selected by silencing Jmjd3. Results: Q-PCR and immunofluorescence revealed that the expression of Jmjd3 was increased in inflamed HPDLs. Knockdown of Jmjd3 led to the suppression of inflammation-induced up-regulation of interleukin-6 and interleukin-12b. Conclusion: Jmjd3 regulated the activation of interleukin-6 and interleukin-12b in the inflammatory response of HPDLs.

Key words: Jumonji domain-containing protein 3, human periodontal ligament cells, inflammation

王璞玉, 索来. Jumonji结构域蛋白3调节人牙周膜细胞的炎症反应[J]. 口腔医学研究, 2022, 38(5): 424-428.

WANG Puyu, SUO Lai. Jumonji Domain-containing Protein 3 Regulates the Inflammatory Response of Human Periodontal Ligament Cells[J]. Journal of Oral Science Research, 2022, 38(5): 424-428.

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Jumonji结构域蛋白3调节人牙周膜细胞的炎症反应

Jumonji Domain-containing Protein 3 Regulates the Inflammatory Response of Human Periodontal Ligament Cells

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