口腔医学研究

口腔医学研究 ›› 2015, Vol. 31 ›› Issue (1): 4-6.

• 基础研究论著 • 上一篇    下一篇

hBMP-7基因修饰犬骨髓基质干细胞复合珊瑚羟基磷灰石修复下颌骨缺损

汪卫国*,李伟奇,邓咏华,朱毅男   

  1. 长沙市口腔医院口腔修复科 湖南 长沙 410003
  • 收稿日期:2014-03-11 出版日期:2015-01-28 发布日期:2016-04-29
  • 通讯作者: 汪卫国,E-mail:52359292@qq.com
  • 作者简介:汪卫国(1973~ ),男,湖北人,副主任医师,硕士,研究方向主要为骨组织工程,口腔种植。原单位是解放军163医院。
  • 基金资助:
    全军科学技术研究"十一五"计划课题项目(编号:06Q029)

Construction of hBMP-7 Gene Recombinant Adenovirus and Its Expression after Infecting dMSCs.

WANG Wei-guo, LI Wei-qi, DENG Yong-hua, et al   

  1. Changsha Stomatological Hospital, Changsha 410005
  • Received:2014-03-11 Online:2015-01-28 Published:2016-04-29

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摘要： 目的:构建携带人骨形态发生蛋白7(hBMP-7)基因的重组腺病毒载体,体外转染犬骨髓基质干细胞(dMSCs)复合珊瑚羟基磷灰石(coral hydroxyapatite, CHA),观察其修复下颌骨缺损的效果。方法:利用AdEasy腺病毒表达系统,体外构建高效表达hBMP-7重组腺病毒载体,转染dMSCs 与珊瑚羟基磷灰石支架材料复合,再分别植入取材动物的自体下颌骨缺损区,分别于4周和8周取材观察成骨情况。结果:大体观察、X线检查及组织学观察均发现构建组织工程骨在祼鼠皮下及骨缺损处均有明显新骨组织形成。结论:构建携带人骨形态发生蛋白7(hBMP-7)基因的重组腺病毒载体,体外转染dMSCs后复合珊瑚羟基磷灰石有较多量骨组织形成,可有效修复下颌骨缺损。[关键词] 人骨形态发生蛋白7 骨髓基质干细胞 腺病毒 组织工程

关键词: 人骨形态发生蛋白7, 骨髓基质干细胞, 腺病毒, 组织工程

Abstract: Objective: To observe the repair of mandibular defects with tissue engineering bone using coral hydroxyapatite and dog marrow stromal cells (dMSCs) which was transfected by recombinant adenovirus vector in vitro carrying human bone morphogenetic protein-7 (hBMP-7) gene. Methods: After the compound of coral hydroxyapatite composite scaffold material and dMSCs transfected with high-level expression hBMP-7 adenovirus vector constructed by AdEasy adenovirus expression system was implanted in mandibular defects of dogs, the reparation situation of mandibular defection was observed 4 weeks and 8 weeks after operation respectively. Results: Significant new bone formation was found in the naked mouse skin and bone defect by gross observation, X-ray examination and histological observation of the constructed tissue engineering bone. Conclusion: Constructed tissue engineering bone carrying hBMP-7 gene was found to format more volume bone tissue, and could effectively repair mandibular defect.

Key words: Human bone morphogenetic protein 7, Marrow stromal cells, Adenovirus , Tissue engineering

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