P120ctn基因沉默及过表达对口腔鳞癌细胞迁移和侵袭的影响

doi:10.13701/j.cnki.kqyxyj.2016.11.007

口腔医学研究 ›› 2016, Vol. 32 ›› Issue (11): 1141-1145.DOI: 10.13701/j.cnki.kqyxyj.2016.11.007

P120^ctn基因沉默及过表达对口腔鳞癌细胞迁移和侵袭的影响

陈钟^*,纪晴,郑晓丹

厦门医学院口腔医学系福建厦门 361008

收稿日期:2016-05-09 出版日期:2016-11-25 发布日期:2016-11-25
通讯作者: 陈钟,E-mail:310571932@qq.com
作者简介:陈钟(1975~ ),女,贵州人,博士,副教授,主要从事口腔鳞癌的淋巴结转移研究。
基金资助:
厦门医学院自然科学项目(编号:Z2013-02)

Effect of P120^ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma

CHEN Zhong, JI Qing, ZHENG Xiao-dan

Department of Stomatology, Xiamen Medical College, Xiamen 361008, China

Received:2016-05-09 Online:2016-11-25 Published:2016-11-25

摘要/Abstract

摘要： 目的:利用舌癌原发灶和转移淋巴结细胞系HN4和HN12,通过沉默和过表达P120连环蛋白(P120-catenin,P120^ctn)基因,观察P120ctn的表达对E-钙黏蛋白(E-cadherin, E-cad)表达和口腔鳞癌迁移和侵袭能力的影响。方法:采用质粒pGFP-V-RS-P120ctn shRNA转染P120ctn高表达的HN4细胞,使HN4中P120ctn的表达显著降低,采用质粒pCMV6-AC-GFP- P120^ctn转染HN12,使HN12过表达P120^ctn,进行P120^ctn和E-cad mRNA和蛋白水平的检测,并通过Transwell细胞迁移及侵袭试验检测转染前后肿瘤细胞迁移和侵袭能力的变化。结果:用质粒转染HN4细胞后发现,随着P120ctn表达的显著降低,E-cad的mRNA和蛋白表达水平明显降低,细胞迁移和侵袭能力显著提高。反之,HN12细胞被转染后发现,过表达P120^ctn的HN12细胞E-cad的蛋白表达水平也显著提高,细胞迁移和侵袭能力显著降低(P＜0.05)。 结论:在OSCC细胞系中P120ctn的表达与E-cad相关,可能参与了细胞粘附的调控,进而在口腔鳞癌细胞的迁移和侵袭过程中发挥作用。

关键词: P120-连环蛋白, E-钙黏蛋白, 口腔鳞状细胞癌

Abstract: Objective: To investigate the effect of P120^ctn silence and overexpression on cell migration and invasion of oral squamous cell cancer (OSCC). Methods: Plasmid pGFP-V-RS-P120^ctn shRNA was used to transfect HN4 cells, and plasmid pCMV6-AC-GFP-P120^ctn was used to transfect HN12 cells. Real-time PCR and Western Blot were performed to detect the mRNA and protein expression levels of P120^ctn and E-cad in OSCC cells before and after transfection. Trans-well assays were used to detect the invasion and migration abilities of OSCC cells before and after transfection. Results: After plasmid pGFP-V-RS-P120ctn shRNA transfection in HN4 cells, the expression levels of P120^ctn and E-cad were significantly decreased, while cell migration and invasion abilities were significantly increased (P<0.05). After plasmid pCMV6-AC-GFP-P120^ctn transfection in HN12 cells, the expression levels of P120^ctn and E-cad were significantly increased, while cell migration and invasion abilities were significantly decreased (P<0.05). Conclusion: P120^ctn may be involved in the invasion and metastasis by regulating the expression of E-cad and cell adhesion in OSCC.

Key words: P120-catenin, E-cadherin, Oral squamous cell cancer

中图分类号:

R739.8

陈钟,纪晴,郑晓丹. P120^ctn基因沉默及过表达对口腔鳞癌细胞迁移和侵袭的影响[J]. 口腔医学研究, 2016, 32(11): 1141-1145.

CHEN Zhong, JI Qing, ZHENG Xiao-dan. Effect of P120^ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma[J]. Journal of Oral Science Research, 2016, 32(11): 1141-1145.

参考文献

[1] Ishiyama N, Lee SH, Liu S, et al. Dynamic and static interactions between p120 catenin and E-cadherin regulate the stability of cell-cell adhesion [J]. Cell, 2010, 141(1)∶117-128
[2] Kourtidis A, Ngok SP, Anastasiadis PZ. p120 catenin: an essential regulator of cadherin stability, adhesion-induced signaling, and cancer progression [J]. Prog Mol Biol Transl Sci, 2013, 116(4)∶409-432
[3] Smalley-Freed WG, Efimov A, Burnett PE, et al. P120-catenin is essential for maintenance of barrier function and intestinal homeostasis in mice [J]. J Clin Invest, 2010,120(6)∶1824-1835
[4] Hanahan D, Weinberg RA. Hallmarks of cancer: the next generation [J]. Cell, 2011, 144(5)∶646-674
[5] 巫家晓,罗婷婷,于在海,等.口腔鳞癌转移与特异性核基质结合区结合蛋白1表达的关系[J].口腔医学研究,2014,30(5)∶438-440
[6] Wang H, Patel V, Miyazaki H, et al. Role for EPS8 in squamous carcinogenesis [J]. Carcinogenesis, 2009, 30(1)∶165-174
[7] Yeudall WA, Miyazaki H, Ensley JF, et al. Uncoupling of epidermal growth factor dependent proliferation and invasion in a model of squamous carcinoma progression [J]. Oral Oncol, 2005, 41(7)∶698-708
[8] Xiong H, Hong J, Du W, et al. Roles of STAT3 and ZEB1 proteins in E-cadherin down-regulation and human colorectal cancer epithelial-mesenchymal transition [J]. J Biol Chem, 2012,287(8)∶5819-5832
[9] Wheelock MJ, Johnson KR. Cadherins as modulators of cellular phenotype [J]. Annu Rev Cell Dev Biol, 2003, 19(1)∶207-235
[10] Thoreson MA, Anastasiadis PZ, Daniel JM, et al. Selective uncoupling of p120(ctn) from E-cadherin disrupts strong adhesion [J]. J Cell Biol, 2000, 148(1)∶189-202
[11] Schackmann RC, Klarenbeek S, Vlug EJ, et al. Loss of p120-catenin induces metastatic progression of breast cancer by inducing anoikis resistance and augmenting growth factor receptor signaling [J]. Cancer Res, 2013,73(15)∶4937-4949
[12] Antonis Kourtidis, Masahiro Yanagisawa, Deborah Huveldt, et al. Pro-tumorigenic phosphorylation of p120 catenin in renal and breast cancer [J]. Plos one, 2015, 10(6)∶e0129964
[13] Lo Muzio L, Pannone G, Santarelli A, et al. Is expression of p120^ctn in oral squamous cell carcinomas a prognostic factor [J]? Oral Surg Oral Med Oral Pathol Oral Radiol, 2013,115(6)∶789-798
[14] 陈钟,董孟华,邓锋.PKC、P120ctn和E- cad在口腔鳞癌中的表达[J].实用口腔医学杂志,2014,30(1)∶61-65
[15] Davis MA, Ireton RC, Reynolds AB. A core function for p120-catenin in cadherin turnover [J]. J Cell Biol, 2003, 163(3)∶525-534
[16] Stairs DB, Bayne LJ, Rhoades B, et al. Deletion of p120-catenin results in a tumor microenvironment with inflammation and cancer that establishes it as a tumor suppressor gene [J]. Cancer Cell, 2011,19(3)∶470-483
[17] Pieters T, van Hengel J, van Roy F. Functions of p120ctn in development and disease [J]. Front. Biosci, 2012, 17(5)∶760-783
[18] Natalia A, Bulgakova, Nicholas H. Brown. Drosophila p120-catenin is crucial for endocytosis of the dynamic E-cadherin-Bazooka complex [J]. Journal of Cell Science, 2016, 129(3)∶477-482
[19] Maria P. Foschini, Elisa Leonardi, Leonardo Henry Eusebi, et al. Podoplanin and E-cadherin expression in preoperative incisional biopsies of oral squamous cell carcinoma is related to lymph node metastases [J]. Int J Surg Patho, 2013, 21(2)∶133-141
[20] Dan Zhao, Xiu-Fa Tang, Kai Yang. Over-expression of integrin-linked kinase correlates with aberrant expression of Snail, E-cadherin and N-cadherin in oral squamous cell carcinoma: implications in tumor progression and metastasis [J]. Clin Exp Metastasis, 2012, 29(8)∶957-969

P120^ctn基因沉默及过表达对口腔鳞癌细胞迁移和侵袭的影响

Effect of P120^ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma

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P120ctn基因沉默及过表达对口腔鳞癌细胞迁移和侵袭的影响

Effect of P120ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma

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P120^ctn基因沉默及过表达对口腔鳞癌细胞迁移和侵袭的影响

Effect of P120^ctn Silence and Overexpression on Cell Migration and Invasion of Oral Squamous Cell Carcinoma